These mice possess loxP sites flanking exons 4-14 of the tripartite motif-containing 28 (Trim28) gene and have applications in studies related to epigenetic modification, pre- implantation embryonic development, and transcriptional repression.
Dr. Barbara Knowles, Institute of Medical Biology
Pierre Chambon, IGBMC/GIE-CERBM
The Trim28 gene encodes a protein that acts as a transcriptional corepressor, with an important role in chromatin remodeling, genomic imprinting and DNA methylation in pre-implantation development, as well as thymocyte development regulation. These mice possess loxP sites on either side of exons 4 through 14 of the targeted Trim28 gene. Mice that are homozygous for this allele are viable and fertile. When these mutant mice are bred to mice that express Cre recombinase, resulting offspring will have exons 4 through 14 deleted in the cre-expressing tissues.
When bred to a strain with Cre recombinase expression in oocytes (see Stock No. 003651 for example), this mutant mouse strain may be useful in studies of pre-implantation embryonic development.
A targeting vector designed by Drs. Pierre Chambon (Institut de Genetique et de Biologie Moleculaire et Cellulaire) containing a loxP site flanked PGK-NEO cassette was utilized in the construction of this mutant. This selection cassette was inserted upstream of exon 4 of the targeted gene, and another loxP site was inserted downstream of exon 14. This construct was electroporated into 129S2/SvPas derived H1 embryonic stem (ES) cells.
Correctly targeted ES cells were injected into C57BL/6 blastocysts. The resulting chimeric animals were crossed to transgenic mice expressing Cre recombinase under the control of the cytomegalovirus promoter (on a background including B6 and SJL). The mice that retained loxP site flanked exons 4 through 14 were then backcrossed to C57BL/6J for 10 generations. Upon arrival at The Jackson Laboratory, the mice were crossed to C57BL/6J (Stock No. 000664) at least once to establish the colony.
|Allele Name||targeted mutation 1.1, Pierre Chambon|
|Allele Type||Targeted (Conditional ready (e.g. floxed), No functional change)|
|Allele Synonym(s)||Kap1fl; L2; Trim28f|
|Gene Symbol and Name||Trim28, tripartite motif-containing 28|
|Strain of Origin||129S2/SvPas|
|Molecular Note||Insertion of loxP sites as follows: A PGK-neomycin cassette flanked by two loxP sites was introduced into intron 3 and a loxP site was introduced into intron 14. Mice positive for the presence of this allele were mated to CMV-Cre transgenic mice to generate mice in which the PGK-neomycin cassette sequences were deleted.|
When maintaining a live colony, these mice can be bred as homozygotes.
When using the Tif1b- mouse strain in a publication, please cite the originating article(s) and include JAX stock #018552 in your Materials and Methods section.