B6.Cg-Tg(Csf1r-EGFP)1Hume/J

Stock number: 018549
Product name: MacGreen
Research areas: Research Tools

Description

These 7.2fms-EGFP transgenic mice express enhanced green fluorescent protein in macrophages and trophoblast cells. This GFP reporter transgene may be used to label mononuclear phagocyte lineage cells.

Detailed description

These MacGreen transgenic mice express the EGFP, enhanced green fluorescent protein, under the control of the mouse Csf1r, colony stimulating factor 1 receptor, promoter. Transgene expression (EGFP positive cells) mimics endogenous gene expression patterns. Fluorescence is detected in peritoneal, bone marrow-derived, and broncho/alveolar lavage macrophages, as well as Langerhans cells, by FACS analysis. At embryonic day 7.5, fluorescence is detected in the ectoplacental cone area. Trophoblasts in the deciduum and trophoblastic giant cells fluoresce in embryos aged embryonic day 10.5 and 12.5. At embryonic day 9.5, yolk sac-derived embryonic phagocytes are detectable by direct fluorescence microscopy. Mice hemizygous for the transgenic insert are viable and fertile. The Donating Investigator reports that homozygous mice have compromised fertility.

Development

Dr. David Hume, while at University of Queensland, Australia, designed a the p7.2fms-EGFP transgenic construct containing EGFP, enhanced green fluorescent protein, under the control of the 7.2-kb mouse Csf1r, colony stimulating factor 1 receptor, promoter, which was injected into fertilized (C57BL/6 X CBA)F1 mouse eggs. The resulting transgenic mice were bred to C57BL/6 mice. The donating investigator reported that these mice were backcrossed to C57BL/6NCrl for 10 generations (see SNP note below). Upon arrival at The Jackson Laboratory, the mice were crossed to C57BL/6NJ (Stock No. 005304) at least once to establish the colony.

A 48 SNP (single nucleotide polymorphism) panel analysis, with 43 markers covering all 19 chromosomes and the X chromosome, as well as 5 markers that distinguish between the C57BL/6J and C57BL/6N substrains, was performed on the rederived living colony at The Jackson Laboratory Repository. While the 43 markers throughout the genome suggested a C57BL/6 genetic background, 3 of 5 markers that determine C57BL/6J from C57BL/6N were found to be segregating. These data suggest the mice sent to The Jackson Laboratory Repository were on a mixed C57BL/6J ; C57BL/6N genetic background.

Allele

Symbol: Tg(Csf1r-EGFP)1Hume
Name: transgene insertion 1, David A Hume
MGI accession ID: MGI:4420302
Generation method: Transgenic
Attributes: Reporter
Marker symbol: Tg(Csf1r-EGFP)1Hume
Marker name: transgene insertion 1, David A Hume
Chromosome: UN
Strain of origin: (C57BL/6 x CBA)F1
Expressed genes: GFP,Green Fluorescent Protein,
Site of expression: Transgene expression (EGFP positive cells) mimics endogenous gene expression patterns. Fluorescence is detected in peritoneal, bone marrow-derived, and broncho/alveolar lavage macrophages, as well as Langerhans cells by FACS analysis. At embryonic day 7.5, fluorescence is detected in the ectoplacental cone area. Trophoblasts in the deciduum and trophoblastic giant cells fluoresce in embryos aged embryonic day 10.5 and 12.5. At embryonic day 9.5, yolk sac-derived embryonic phagocytes are detectable by direct fluorescence microscopy.
Molecular note: The construct had 3.5 kb of sequence flanking exon 2 and the entire intron 2 sequence of the Csf1r gene driving expression of EGFP. This construct was injected into pronuclei of (C57BL/6 x CBA)F1 fertilized eggs. 5 lines that transmitted the transgene were generated, and EGFP expression was readily detected in peritoneal, bone marrow-derived, and broncho-alveolar lavage macrophages from all lines.