Removal of this mouse colony is imminent. If live mice are needed for your studies, it is advised that they be ordered immediately. After removal, the mice will be available from cryorecovery.
These 7.2fms-EGFP transgenic mice express enhanced green fluorescent protein in macrophages and trophoblast cells. This GFP reporter transgene may be used to label mononuclear phagocyte lineage cells.David Hume, University of Edinburgh
Genetic Background | Generation |
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N10+pN3F4
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Allele Type |
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Transgenic (Reporter) |
Starting at:
$255.00 Domestic price for female |
333.51 Domestic price for breeder pair |
$2,854.50 Domestic price Cryo Recovery |
These MacGreen transgenic mice express the EGFP, enhanced green fluorescent protein, under the control of the mouse Csf1r, colony stimulating factor 1 receptor, promoter. Transgene expression (EGFP positive cells) mimics endogenous gene expression patterns. Fluorescence is detected in peritoneal, bone marrow-derived, and broncho/alveolar lavage macrophages, as well as Langerhans cells, by FACS analysis. At embryonic day 7.5, fluorescence is detected in the ectoplacental cone area. Trophoblasts in the deciduum and trophoblastic giant cells fluoresce in embryos aged embryonic day 10.5 and 12.5. At embryonic day 9.5, yolk sac-derived embryonic phagocytes are detectable by direct fluorescence microscopy. Mice hemizygous for the transgenic insert are viable and fertile. The Donating Investigator reports that homozygous mice have compromised fertility.
Dr. David Hume, while at University of Queensland, Australia, designed a the p7.2fms-EGFP transgenic construct containing EGFP, enhanced green fluorescent protein, under the control of the 7.2-kb mouse Csf1r, colony stimulating factor 1 receptor, promoter, which was injected into fertilized (C57BL/6 X CBA)F1 mouse eggs. The resulting transgenic mice were bred to C57BL/6 mice. The donating investigator reported that these mice were backcrossed to C57BL/6NCrl for 10 generations (see SNP note below). Upon arrival at The Jackson Laboratory, the mice were crossed to C57BL/6NJ (Stock No. 005304) at least once to establish the colony.
A 48 SNP (single nucleotide polymorphism) panel analysis, with 43 markers covering all 19 chromosomes and the X chromosome, as well as 5 markers that distinguish between the C57BL/6J and C57BL/6N substrains, was performed on the rederived living colony at The Jackson Laboratory Repository. While the 43 markers throughout the genome suggested a C57BL/6 genetic background, 3 of 5 markers that determine C57BL/6J from C57BL/6N were found to be segregating. These data suggest the mice sent to The Jackson Laboratory Repository were on a mixed C57BL/6J ; C57BL/6N genetic background.
Expressed Gene | GFP, Green Fluorescent Protein, |
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Site of Expression | Transgene expression (EGFP positive cells) mimics endogenous gene expression patterns. Fluorescence is detected in peritoneal, bone marrow-derived, and broncho/alveolar lavage macrophages, as well as Langerhans cells by FACS analysis. At embryonic day 7.5, fluorescence is detected in the ectoplacental cone area. Trophoblasts in the deciduum and trophoblastic giant cells fluoresce in embryos aged embryonic day 10.5 and 12.5. At embryonic day 9.5, yolk sac-derived embryonic phagocytes are detectable by direct fluorescence microscopy. |
Allele Name | transgene insertion 1, David A Hume |
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Allele Type | Transgenic (Reporter) |
Allele Synonym(s) | 7.2fms-EGFP; c-fms-EGFP; Csf1r-EGFP; MacGreen; Tg(Csf1r-Gfp)Hume |
Gene Symbol and Name | Tg(Csf1r-EGFP)1Hume, transgene insertion 1, David A Hume |
Gene Synonym(s) | |
Promoter | Csf1r, colony stimulating factor 1 receptor, mouse, laboratory |
Expressed Gene | GFP, Green Fluorescent Protein, |
Site of Expression | Transgene expression (EGFP positive cells) mimics endogenous gene expression patterns. Fluorescence is detected in peritoneal, bone marrow-derived, and broncho/alveolar lavage macrophages, as well as Langerhans cells by FACS analysis. At embryonic day 7.5, fluorescence is detected in the ectoplacental cone area. Trophoblasts in the deciduum and trophoblastic giant cells fluoresce in embryos aged embryonic day 10.5 and 12.5. At embryonic day 9.5, yolk sac-derived embryonic phagocytes are detectable by direct fluorescence microscopy. |
Strain of Origin | (C57BL/6 x CBA)F1 |
Chromosome | UN |
Molecular Note | The construct had 3.5 kb of sequence flanking exon 2 and the entire intron 2 sequence of the Csf1r gene driving expression of EGFP. This construct was injected into pronuclei of (C57BL/6 x CBA)F1 fertilized eggs. 5 lines that transmitted the transgene were generated, and EGFP expression was readily detected in peritoneal, bone marrow-derived, and broncho-alveolar lavage macrophages from all lines. |
When maintaining a live colony, hemizygous mice may be bred together, to wildtype siblings, or to C57BL/6NJ inbred mice (Stock No. 005304). The Donating Investigator reports that homozygous mice have compromised fertility.
When using the MacGreen mouse strain in a publication, please cite the originating article(s) and include JAX stock #018549 in your Materials and Methods section.
Service/Product | Description | Price |
---|---|---|
Hemizygous or Non carrier for Tg(Csf1r-EGFP)1Hume |
Frozen Mouse Embryo | B6.Cg-Tg(Csf1r-EGFP)1Hume/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6.Cg-Tg(Csf1r-EGFP)1Hume/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6.Cg-Tg(Csf1r-EGFP)1Hume/J Frozen Embryo | $3373.50 |
Frozen Mouse Embryo | B6.Cg-Tg(Csf1r-EGFP)1Hume/J Frozen Embryo | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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