This CAG-CAT-Twist1 transgenic line expresses mouse Twist1, twist homolog 1 (Drosophila), gene in a Cre recombinase inducible fashion, and has applications in studies of craniofacial development, cartilage formation, endochondral ossification and heart valve development.
Douglas B Spicer, University of New England
The TWIST1 basic helix loop helix transcription factor, plays a role in heart and bone development, as well as lineage specification, differentiation and tumorigenesis. Mutations of TWIST1 in humans are associated with Saethre-Chotzen syndrome and Robinow-Sorauf syndrome. When these CAG-CAT-Twist1 transgenic mice are crossed with a Cre recombinase-expressing strain, the floxed (cat) chloramphenicol acetyltransferase gene is deleted and the CAG promoter drives expression of the mouse Twist1, twist homolog 1 (Drosophila), gene in the cre-expressing cells. The floxed (cat) chloramphenicol acetyltransferase gene serves as a reporter/silencer, preventing expression of Twist1 in the absence of Cre recombinase activity, and as a reporter for transgenic expression.Mice homozygous for the transgenic insert are viable, fertile and exhibit no overt phenotype. The Donating Investigator reports that there are no phenotypic differences between hemizygotes and homozygotes.
When bred to a strain with Cre recombinase expression in neural crest cells (see Stock No. 003829 for example), this mutant mouse strain may be useful in studies of craniofacial development.
When bred to a strain with Cre recombinase expression in endothelial cells (see Stock No. 004128 for example), this mutant mouse strain may be useful in studies of heart valve development.
When bred to a strain with Cre recombinase expression in chondrocytes (see Stock No. 003554 for example), this mutant mouse strain may be useful in studies of ossification, growth plate development, and dwarfism.
A transgenic construct containing a chloramphenicol acetyltransferase (cat) gene flanked by loxP sites, upstream of the Twist1, twist homolog 1 (Drosophila), ORF, under the control of the CAG, CMV enhancer/chicken beta-actin promoter, was injected into fertilized FVB mouse eggs. Founder line 57 was subsequently established. The strain was maintained on the FVB background by the Donating Investigator. Upon arrival at The Jackson Laboratory, the mice were crossed to FVB/NJ (Stock No. 001800) at least once to establish the colony.
|Expressed Gene||Twist1, twist basic helix-loop-helix transcription factor 1, mouse, laboratory|
|Site of Expression|
|Allele Name||transgene insertion 1, Douglas B Spicer|
|Allele Type||Transgenic (Inserted expressed sequence)|
|Allele Synonym(s)||CAG-CAT-Twist; CAGCAT-Twist1; CC-Twist|
|Gene Symbol and Name||Tg(CAG-cat,-Twist1)1Dbsp, transgene insertion 1, Douglas B Spicer|
|Promoter||CMV, cytomegalovirus, human|
|Expressed Gene||Twist1, twist basic helix-loop-helix transcription factor 1, mouse, laboratory|
|Strain of Origin||FVB|
|Molecular Note||The CAG promoter drives expression of a floxed chloramphenicol acetyltransferase gene and the mouse Twist1 gene. Chloramphenicol acetyltransferase serves as a reporter and a silencer. Twist1 expression occurs only after cre excision of chloramphenicol acetyltransferase.|
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