Exon 1 of the X-linked androgen receptor (Ar) gene is floxed, making this conditional mutant strain useful when studying male sexual development and fertility.
Robert E Braun, The Jackson Laboratory
Genetic Background | Generation |
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|
Allele Type | Gene Symbol | Gene Name |
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Targeted (Conditional ready (e.g. floxed), No functional change) | Ar | androgen receptor |
These mice possess loxP sites flanking exon 1 of the X-linked androgen receptor (Ar) gene. AR is a nuclear transcription factor expressed in Sertoli cells which becomes activated by binding to testosterone or dihydrotestosterone. AR is required for male embryonic sexual differentiation, the development of male sexual characteristics, and the regulation of spermatogenesis. Homozygous females and hemizygous males are viable and fertile. When these mutant mice are bred to mice that express Cre recombinase, resulting offspring will have exon 1 deleted in cre-expressing tissues, abolishing gene function.
For example, breeding these mice to 129S.FVB-Tg(Amh-cre)8815Reb/J mice (Stock No. 007915), results in the production of a new variety of Sertoli cell AR knock out (SCARKO) mice. These mice are azoospermic and show a block in meiosis in Sertoli cells, with normal expression of Ar in Leydig cells and myoid epithelial cells.
In an attempt to offer alleles on well-characterized or multiple genetic backgrounds, alleles are frequently moved to a genetic background different from that on which an allele was first characterized. It should be noted that the phenotype could vary from that originally described. We will modify the strain description if necessary as published results become available.
A targeting vector was designed to insert a single loxP site upstream of exon 1, and a frt-flanked neomycin resistance (neo) cassette followed by another loxP site downstream of exon 1 of the androgen receptor (Ar) gene. The construct was electroporated into 129S1/SvImJ-derived embryonic stem (ES) cells. Correctly targeted ES cells were transiently transfected with a CMV-Flp expression plasmid to delete the neo cassette. Correctly targeted ES cells, lacking neo, were injected into C57BL/6J blastocysts and resulting chimeric males were bred with 129S1/SvImJ females (Stock No. 002448) to establish a colony. Upon arrival, mice were bred to 129S1/SvImJ inbred mice for at least one generation.
Allele Name | targeted mutation 2.1, Robert E Braun |
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Allele Type | Targeted (Conditional ready (e.g. floxed), No functional change) |
Allele Synonym(s) | |
Gene Symbol and Name | Ar, androgen receptor |
Gene Synonym(s) | |
Strain of Origin | 129S1/SvImJ |
Chromosome | X |
Molecular Note | A targeting vector was designed to insert a single loxP site upstream of exon 1 and the Ar start site, and a frt-flanked neomycin resistance (neo) cassette, followed by another loxP site, downstream of exon 1. Flp-mediated recombination removed the neo cassette. |
When maintaining a live colony, homozygous females may be bred to hemizygous males.
When using the 129S1/SvImJ-Artm2.1Reb/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #018451 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
X linked - Heterozygous females and non carrier males for Ar<tm2.1Reb>, 1 pair minimum |
Frozen Mouse Embryo | 129S1/SvImJ-Ar<tm2.1Reb>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | 129S1/SvImJ-Ar<tm2.1Reb>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | 129S1/SvImJ-Ar<tm2.1Reb>/J Frozen Embryo | $3373.50 |
Frozen Mouse Embryo | 129S1/SvImJ-Ar<tm2.1Reb>/J Frozen Embryo | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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