C57BL/6N-Tg(SNCA)129Mjff/J

Stock number: 018442
Product name: MJFF-9 SNCA WT line 129
Research areas: Neurobiology Research

Description

These SNCA-Wt mice express a wild-type human synuclein, alpha (non A4 component of amyloid precursor), SNCA, gene directed by the human SNCA promoter/enhancer regions on the BAC transgene. These mice may be useful for studying SNCA function and the pathogenesis of Parkinson's disease.

Detailed description

Mice hemizygous for the BAC SNCA-Wt transgene are viable and fertile. These mice express a wild-type human synuclein, alpha (non A4 component of amyloid precursor), SNCA, gene directed by the human SNCA promoter/enhancer regions on the BAC transgene. The Donating Investigator reports that using qRT-PCR and Western Blot analysis, transgene expression is detected in the cortex and hippocampus, but not in kidney.

The Donating Investigator has not attempted to make this strain homozygous, and reports that preliminary data indicate that there is one copy of the transgene. Further characterization and verification of transgene expression is forthcoming.

Development

The human bacterial artificial chromosome (BAC) RP1-458H10, containing the entire synuclein, alpha (non A4 component of amyloid precursor), SNCA, gene was used as the source for the SNCA-Wt transgene, which was linearized and microinjected into the pronucleus of C57BL/6NTac fertilized eggs. Males from founder line 129 were bred to C57BL/6NTac females to establish the colony. The mice were then backcrossed to C57BL/6NTac for 2 generations. Upon arrival, transgenic mice were bred with C57BL/6NJ inbred mice (Stock No. 005304) for at least one generation to establish the colony.

Allele

Symbol: Tg(SNCA)129Mjff
Name: transgene insertion 129, The Michael J Fox Foundation
MGI accession ID: MGI:5424882
Generation method: Transgenic
Attributes: Inserted expressed sequence; Humanized sequence
Synonyms: MJFF-9 SNCA WT line 129
Marker symbol: Tg(SNCA)129Mjff
Marker name: transgene insertion 129, The Michael J Fox Foundation
Chromosome: 7
Strain of origin: C57BL/6NTac
Expressed genes: SNCA,synuclein alpha,human
Site of expression: Cortex and hippocampus.
Molecular note: The human bacterial artificial chromosome (BAC) RP1-458H10, containing the entire synuclein, alpha (non A4 component of amyloid precursor), SNCA, gene was used as the source for the SNCA-Wt transgene, which was linearized and microinjected into the pronucleus of C57BL/6NTac fertilized eggs. Transgene insertion occurred on Chr 7, causing a 898 bp deletion.