These SNCA-Wt mice express a wild-type human synuclein, alpha (non A4 component of amyloid precursor), SNCA, gene directed by the human SNCA promoter/enhancer regions on the BAC transgene. These mice may be useful for studying SNCA function and the pathogenesis of Parkinson's disease.
Kuldip Dave, The Michael J. Fox Foundation
Genetic Background | Generation |
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Allele Type |
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Transgenic (Inserted expressed sequence, Humanized sequence) |
Mice hemizygous for the BAC SNCA-Wt transgene are viable and fertile. These mice express a wild-type human synuclein, alpha (non A4 component of amyloid precursor), SNCA, gene directed by the human SNCA promoter/enhancer regions on the BAC transgene. The Donating Investigator reports that using qRT-PCR and Western Blot analysis, transgene expression is detected in the cortex and hippocampus, but not in kidney.
The Donating Investigator has not attempted to make this strain homozygous, and reports that preliminary data indicate that there is one copy of the transgene. Further characterization and verification of transgene expression is forthcoming.
The human bacterial artificial chromosome (BAC) RP1-458H10, containing the entire synuclein, alpha (non A4 component of amyloid precursor), SNCA, gene was used as the source for the SNCA-Wt transgene, which was linearized and microinjected into the pronucleus of C57BL/6NTac fertilized eggs. Males from founder line 129 were bred to C57BL/6NTac females to establish the colony. The mice were then backcrossed to C57BL/6NTac for 2 generations. Upon arrival, transgenic mice were bred with C57BL/6NJ inbred mice (Stock No. 005304) for at least one generation to establish the colony.
Expressed Gene | SNCA, synuclein alpha, human |
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Site of Expression | Cortex and hippocampus. |
Allele Name | transgene insertion 129, The Michael J Fox Foundation |
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Allele Type | Transgenic (Inserted expressed sequence, Humanized sequence) |
Allele Synonym(s) | MJFF-9 SNCA WT line 129 |
Gene Symbol and Name | Tg(SNCA)129Mjff, transgene insertion 129, The Michael J Fox Foundation |
Gene Synonym(s) | |
Promoter | SNCA, synuclein alpha, human |
Expressed Gene | SNCA, synuclein alpha, human |
Site of Expression | Cortex and hippocampus. |
Strain of Origin | C57BL/6NTac |
Chromosome | 7 |
Molecular Note | The human bacterial artificial chromosome (BAC) RP1-458H10, containing the entire synuclein, alpha (non A4 component of amyloid precursor), SNCA, gene was used as the source for the SNCA-Wt transgene, which was linearized and microinjected into the pronucleus of C57BL/6NTac fertilized eggs. Transgene insertion occurred on Chr 7, causing a 898 bp deletion. |
When maintaining a live colony, hemizygous mice may be bred together, to wildtype siblings, or to C57BL/6NJ inbred mice (Stock No. 005304).
When using the MJFF-9 SNCA WT line 129 mouse strain in a publication, please cite the originating article(s) and include JAX stock #018442 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
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Hemizygous or Non Carrier for Tg(SNCA)129Mjff |
Frozen Mouse Embryo | C57BL/6N-Tg(SNCA)129Mjff/J | $2595.00 |
Frozen Mouse Embryo | C57BL/6N-Tg(SNCA)129Mjff/J | $2595.00 |
Frozen Mouse Embryo | C57BL/6N-Tg(SNCA)129Mjff/J | $3373.50 |
Frozen Mouse Embryo | C57BL/6N-Tg(SNCA)129Mjff/J | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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