FVB.Cg-Tg(KRT5-cre/ERT2)2Ipc/JeldJ

Stock number: 018394
Product name: K5-Cre-ER^T2
Research areas: Research Tools

Description

K5-Cre-ERT2 transgenic mice express tamoxifen-inducible Cre recombinase under the direction of the bovine keratin 5 (KRT5) promoter/enhancer regions. They are useful for applications requiring tamoxifen-induced deletion of floxed sequences in epithelial cells.

Of note, a C57BL/6-congenic Krt5-CreERT2 knock-in allele is also available (Stock No. 029155).

Detailed description

Expression in this Cre-ER^T2 transgenic line is directed by the bovine keratin 5 (KRT5) promoter/enhancer regions. These mice express Cre-ER^T2 protein in epithelial cells. Restricted to the cytoplasm, Cre-ER^T2 can only gain access to the nuclear compartment after exposure to tamoxifen. Mice hemizygous for the KRT5-creER^T2 transgene are viable and fertile. When these mice are bred with mice containing loxP-flanked sequence, tamoxifen-inducible, Cre-mediated recombination will result in deletion of the floxed sequences in the Cre-expressing cells of the offspring.

For example, when bred to B6.129(SJL)-Foxn1^tm1.1Dmsu/J mice (Stock No. 012941), the resulting mice develop significant thymic atrophy due to increased apoptosis associated with p53 activation.

 

Development

A transgenic vector was designed to have a bovine Keratin 5 (KRT5) promoter sequence, a rabbit beta-globin intron II sequence, a creER^T2 fusion gene (a Cre recombinase fused to a human estrogen receptor ligand binding domain) and an SV40 polyadenylation sequence. This transgene was microinjected into fertilized C57BL/6 x SJL F1 oocytes. Offspring from founder line 2 have been backcrossed to FVB/NCrl mice for at least 11 generations. Upon arrival at The Jackson Laboratory, mice were bred to FVB/NJ inbred mice (Stock No. 001800) for at least one generation.



A 48 SNP (single nucleotide polymorphism) panel analysis, with 43 markers covering all 19 chromosomes and the X chromosome, as well as 5 markers that distinguish between the C57BL/6J and C57BL/6N substrains, was performed on the rederived living colony at The Jackson Laboratory Repository. One of the 43 markers, on Chromosome 12, was segregating with B6.

Allele

Symbol: Tg(KRT5-cre/ERT2)2Ipc
Name: transgene insertion 2, I Pierre Chambon
MGI accession ID: MGI:2177429
Generation method: Transgenic
Attributes: Recombinase-expressing; Inducible
Synonyms: CreERT2; K5-Cre-ER^T2; K5-CreERT2; Krt5^CreERT2; Tg(Krt1-5-cre/ERT2)2Ipc
Marker symbol: Tg(KRT5-cre/ERT2)2Ipc
Marker name: transgene insertion 2, I Pierre Chambon
Marker synonyms: CreERT2; K5-Cre-ER^T2; K5-CreERT2; Tg(Krt1-5-creERT2)2Ipc
Chromosome: UN
Strain of origin: (C57BL/6 x SJL)F1
Expressed genes: cre,cre recombinase,bacteriophage P1
Site of expression: Cre-ERT2 protein is expressed in epithelial cells.
Molecular note: This transgene expresses a tamoxifen-inducible form of cre recombinase under the control of the bovine KRT5 promoter in epidermal tissues.