These β1glo transgenic mice express cre-inducible, HA tagged TGF-β1. They may be useful in applications related to differentiation and homeostasis during autoimmunity, inflammation, fibrosis and cancer.
Ashok B Kulkarni, NIDCR NIH
Genetic Background | Generation |
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Allele Type |
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Transgenic (Conditional ready (e.g. floxed), Reporter, Constitutively active, Inserted expressed sequence) |
These β1glo mice possess a CMV promoter driving expression of a loxP-flanked EGFP/polyA cassette upstream of a constitutively active HA tagged porcine transforming growth factor, beta 1 (Tgfb1) cDNA. TGF-β1 is a cytokine that regulates cell proliferation and differentiation, apoptosis, and extracellular matrix deposition. These mice show widespread expression of GFP and no TGF-β1 expression from the transgene. Mice that are hemizygous for this allele are viable and fertile. When these mutant mice are bred to mice that express Cre recombinase, resulting offspring will have the floxed EGFP/polyA cassette deleted in cre-expressing tissues. TGF-β1 expression is seen only after the floxed sequence is removed.
For example, when crossed to a strain expressing mouse mammary tumor virus (MMTV) driven Cre recombinase (see Stock No. 003556), expression of TGF-β1 in the secretory cells of the mammary and salivary glands in double mutant mice results in reduced branching and increased mesenchyme in the submandibular salivary gland, as well as hyposalivation in the few pups that survive after birth.
The transgene was designed with (from 5' to 3') the CAG promoter (containing a CMV-IE enhancer/beta-actin promoter), a loxP-flanked enhanced green fluorescent protein (EGFP) and polyA signal, a hemagglutinin (HA)-tagged porcine transforming growth factor, beta 1 (Tgfb1) cDNA, and a 2x-polyA signal. The Tgfb1 cDNA was modified to be constitutively active by missense mutations of two cysteine residues (C223 and C225) to serines. The transgene was microinjected into fertilized FVB/NCrl oocytes, and mice from founder line C8 were backcrossed to FVB/NCrl mice for at least 10 generations. Upon arrival at The Jackson Laboratory, mice were bred to FVB/NJ inbred mice (Stock No. 001800) for at least one generation to establish the colony.
Expressed Gene | GFP, Green Fluorescent Protein, |
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Expressed Gene | TGFB1, transforming growth factor, beta 1, porcine |
Site of Expression | These mice show widespread expression of GFP and no TGF-β1 expression from the transgene until after the floxed EGFP/polyA cassette has been deleted in cre-expressing tissues. |
Allele Name | transgene insertion C8, Ashok B Kulkarni |
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Allele Type | Transgenic (Conditional ready (e.g. floxed), Reporter, Constitutively active, Inserted expressed sequence) |
Allele Synonym(s) | beta1glo; Tg(CAG-EGFP,TGFB1*)C8Akul |
Gene Symbol and Name | Tg(CAG-EGFP,TGFB1*)C8Kul, transgene insertion C8, Ashok B Kulkarni |
Gene Synonym(s) | |
Promoter | CMV, cytomegalovirus, human |
Expressed Gene | GFP, Green Fluorescent Protein, |
Expressed Gene | TGFB1, transforming growth factor, beta 1, porcine |
Site of Expression | These mice show widespread expression of GFP and no TGF-β1 expression from the transgene until after the floxed EGFP/polyA cassette has been deleted in cre-expressing tissues. |
Strain of Origin | FVB/NCrl |
Chromosome | UN |
Molecular Note | The transgene was designed with (from 5' to 3') the CAG promoter (containing a CMV-IE enhancer/beta-actin promoter), a loxP-flanked enhanced green fluorescent protein (EGFP) andpolyA signal, a hemagglutinin (HA)-tagged porcine transforming growth factor, beta 1 (Tgfb1) cDNA, and a 2x-polyA signal. The Tgfb1 cDNA was modified to be constitutively active by missense mutations of two cysteine residues (C223 and C225) to serines. Line C8 was generated. |
Mutations Made By | Ashok Kulkarni, NIDCR NIH |
When maintaining a live colony, hemizygous mice may be bred to wildtype (non-carrier) mice from the colony or FVB/NJ inbred mice (Stock No. 001800).
When using the β1glo mouse strain in a publication, please cite the originating article(s) and include JAX stock #018393 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
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Hemizygous or Non Carrier for Tg(CAG-EGFP,TGFB1*)C8Akul/ |
Frozen Mouse Embryo | FVB/N-Tg(CAG-EGFP,TGFB1*)C8Kul/J | $2595.00 |
Frozen Mouse Embryo | FVB/N-Tg(CAG-EGFP,TGFB1*)C8Kul/J | $2595.00 |
Frozen Mouse Embryo | FVB/N-Tg(CAG-EGFP,TGFB1*)C8Kul/J | $3373.50 |
Frozen Mouse Embryo | FVB/N-Tg(CAG-EGFP,TGFB1*)C8Kul/J | $3373.50 |
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