FVB/N-Tg(CAG-EGFP,TGFB1*)C8Kul/J

Stock number: 018393
Product name: β1^glo
Research areas: Apoptosis Research, Cell Biology Research, Research Tools

Description

These β1^glo transgenic mice express cre-inducible, HA tagged TGF-β1. They may be useful in applications related to differentiation and homeostasis during autoimmunity, inflammation, fibrosis and cancer.

Detailed description

These β1^glo mice possess a CMV promoter driving expression of a loxP-flanked EGFP/polyA cassette upstream of a constitutively active HA tagged porcine transforming growth factor, beta 1 (Tgfb1) cDNA. TGF-β1 is a cytokine that regulates cell proliferation and differentiation, apoptosis, and extracellular matrix deposition. These mice show widespread expression of GFP and no TGF-β1 expression from the transgene. Mice that are hemizygous for this allele are viable and fertile. When these mutant mice are bred to mice that express Cre recombinase, resulting offspring will have the floxed EGFP/polyA cassette deleted in cre-expressing tissues. TGF-β1 expression is seen only after the floxed sequence is removed.

For example, when crossed to a strain expressing mouse mammary tumor virus (MMTV) driven Cre recombinase (see Stock No. 003556), expression of TGF-β1 in the secretory cells of the mammary and salivary glands in double mutant mice results in reduced branching and increased mesenchyme in the submandibular salivary gland, as well as hyposalivation in the few pups that survive after birth.

Development

The transgene was designed with (from 5' to 3') the CAG promoter (containing a CMV-IE enhancer/beta-actin promoter), a loxP-flanked enhanced green fluorescent protein (EGFP) and polyA signal, a hemagglutinin (HA)-tagged porcine transforming growth factor, beta 1 (Tgfb1) cDNA, and a 2x-polyA signal. The Tgfb1 cDNA was modified to be constitutively active by missense mutations of two cysteine residues (C223 and C225) to serines. The transgene was microinjected into fertilized FVB/NCrl oocytes, and mice from founder line C8 were backcrossed to FVB/NCrl mice for at least 10 generations. Upon arrival at The Jackson Laboratory, mice were bred to FVB/NJ inbred mice (Stock No. 001800) for at least one generation to establish the colony.

Allele

Symbol: Tg(CAG-EGFP,TGFB1*)C8Kul
Name: transgene insertion C8, Ashok B Kulkarni
MGI accession ID: MGI:5319386
Generation method: Transgenic
Attributes: Conditional ready (e.g. floxed); Reporter; Constitutively active; Inserted expressed sequence
Marker symbol: Tg(CAG-EGFP,TGFB1*)C8Kul
Marker name: transgene insertion C8, Ashok B Kulkarni
Chromosome: UN
Strain of origin: FVB/NCrl
Expressed genes: TGFB1,transforming growth factor, beta 1,porcine; GFP,Green Fluorescent Protein,
Site of expression: These mice show widespread expression of GFP and no TGF-β1 expression from the transgene until after the floxed EGFP/polyA cassette has been deleted in cre-expressing tissues.
Molecular note: The transgene was designed with (from 5' to 3') the CAG promoter (containing a CMV-IE enhancer/beta-actin promoter), a loxP-flanked enhanced green fluorescent protein (EGFP) and polyA signal, a hemagglutinin (HA)-tagged porcine transforming growth factor, beta 1 (Tgfb1) cDNA, and a 2x-polyA signal. The Tgfb1 cDNA was modified to be constitutively active by missense mutations of two cysteine residues (C223 and C225) to serines. Line C8 was generated.