B6.129S6(SJL)-Ptf1a^{tm3.1(rtTA)Mgn}/Mmjax

Stock number: 018070
Research areas: Developmental Biology Research, Research Tools

Description

This mouse line expresses the reverse tetracycline transactivator (rtTA) under control of the Ptf1a (pancreas specific transcription factor, 1a) regulatory sequences. The donating investigator reports that mice are homozygous lethal.

Detailed description

This mouse line expresses the reverse tetracycline transactivator (rtTA) under control of the Ptf1a (pancreas specific transcription factor, 1a) regulatory sequences. The donating investigator reports that mice are homozygous lethal.

Development

A loxP-flanked targeting vector containing a reverse tetracycline transactivator (rtTA) in the 5' UTR and a frt-flanked hygromycin cassette downstream of exon 2 was inserted into Ptf1a^tm1Mgn (Ptf1a^LCA) by recombinase mediated cassette exchange (RMCE).

rtTA is expressed under control of the endogenous Ptf1a promoter. The construct was electroporated into 129S6/SvEvTac derived TL-1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into blastocysts. The resulting chimeric animals were crossed to B6;SJL-Tg(ACTFLPe)9205Dym/J to remove the hygromycin cassette. Mice were backcrossed to C57BL/6J for 6 generations. Upon arrival, mice were bred to C57BL/6J for at least 1 generation to establish the colony.

Allele

Symbol: Ptf1a^{tm3.1(rtTA)Mgn}
Name: targeted mutation 3.1, Mark A Magnuson
MGI accession ID: MGI:5467924
Generation method: Targeted
Attributes: RMCE-ready; Transactivator
Marker symbol: Ptf1a
Marker name: pancreas specific transcription factor, 1a
Marker synonyms: bHLHa29; p48; PACA; PAGEN2; PTF1-p48
Chromosome: 2
Strain of origin: 129S6/SvEvTac
Expressed genes: rtTA,reverse tetracycline-controlled transactivator,E. coli
Site of expression: rtTA is expressed under control of the endogenous Ptf1a promoter.
Molecular note: A loxP-flanked targeting vector containing a reverse tetracycline transactivator (rtTA) in the 5' UTR and a frt-flanked hygromycin cassette downstream of exon 2 was inserted into the loxed cassette acceptor (LCA) allele by recombinant mediated cassette exchange (RMCE). rtTA is expressed under control of the endogenous Ptf1a promoter. The hygromycin cassette was removed by a flp mediated recombination.