B6;129S4-Srsf1^tm1Xdfu/J

Stock number: 018020
Research areas: Research Tools

Description

These floxed mutant mice possess loxP sites flanking exons 2-4 of the Srsf1 gene. This strain may be useful for generating conditional mutations in applications related to studies of embryonic retinal development.

Detailed description

Srsf1 (serine/arginine-rich splicing factor 1) is a serine/arginine (SR)-rich protein associated with the regulation of constitutive splicing.

These targeted mutation mice carry a floxed allele of the gene. When crossed with a cre strain, tissue specific excision of exons 2-4 can be accomplished.

Ubiquitous excision of the floxed segment is early embryonic lethal. Conditional knockout in cardiomyocytes with a Myl2-cre strain causes postnatal contraction defects and dilated cardiomyopathy.

When bred to mice carrying Tg(Chx10-EGFP/cre,-ALPP)2Clc (see Stock No. 005105 for example) to direct cre recombinase expression to retina and Muller glial cells, this mutant mouse strain may be useful in studies of embryonic retinal development.

Development

A loxP site was placed in intron 1 and a loxP-flanked neomycin resistance cassette was introduced to intron 4 using 129S4/SvJae-derived J1 embryonic stem (ES)cells. Positive ES cell clones were transfected with a cre-expression plasmid to excise the floxed neomycin cassette. The strain was backcrossed to C57BL/6J one time by the donating laboratory.

Allele

Symbol: Srsf1^tm1Xdfu
Name: targeted mutation 1, Xiang-Dong Fu
MGI accession ID: MGI:3530151
Generation method: Targeted
Attributes: Conditional ready (e.g. floxed); No functional change
Marker symbol: Srsf1
Marker name: serine/arginine-rich splicing factor 1
Chromosome: 11
Strain of origin: 129S4/SvJae
Molecular note: A loxP site was inserted upstream of exon 2 and a floxed neo-tk cassette was inserted downstream of exon 4. The neo-tk cassette was removed by transient expression of cre recombinase in correctly targeted ES cells. Resulting mutant animals carry loxP-flanked exons 2-4.