Overview

These floxed mutant mice possess loxP sites flanking exons 2-4 of the Srsf1 gene. This strain may be useful for generating conditional mutations in applications related to studies of embryonic retinal development.

Donating Investigator

Xiang-Dong Fu, University of California, San Diego

Genetic overview

Genetic Background	Generation

Srsf1^tm1Xdfu

Allele Type	Gene Symbol	Gene Name
Targeted (Conditional ready (e.g. floxed), No functional change)	Srsf1	serine/arginine-rich splicing factor 1

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Research Applications

Research Tools

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Base Price

Starting at:

$2,854.50 Domestic price Cryo Recovery

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Details

Detailed Description

Srsf1 (serine/arginine-rich splicing factor 1) is a serine/arginine (SR)-rich protein associated with the regulation of consititive splicing.

These targeted mutation mice carry a floxed allele of the gene. When crossed with a cre strain, tissue specific excision of exons 2-4 can be accomplished.

Ubiquitous excision of the floxed segment is early embryonic lethal. Conditional knockout in cardiomyocytes with a Myl2-cre strain causes postnatal contraction defects and dilated cardiomyopathy.

When bred to mice carrying Tg(Chx10-EGFP/cre,-ALPP)2Clc (see Stock No. 005105 for example) Cre recombinase expression in retina and Muller glial cells, this mutant mouse strain may be useful in studies of embryonic retinal development.

Development

A loxP site was placed in intron 1 and a loxP-flanked neomycin resistance cassette was introduced to intron 4 using 129S4/SvJae-derived J1 embryonic stem (ES)cells. Positive ES cell clones were transfected with a cre-expression plasmid to excise the floxed neomycin cassette. The strain was backcrossed to C57BL/6J one time by the donating laboratory.

Control Suggestions

Approximate Controls

000664 C57BL/6J

Additional Information

Considerations for Choosing Controls

Selected References

Xu X; Yang D; Ding JH; Wang W; Chu PH; Dalton ND; Wang HY; Bermingham JR Jr; Ye Z; Liu F; Rosenfeld MG; Manley JL; Ross J Jr; Chen J; Xiao RP; Cheng H; Fu XD. 2005. ASF/SF2-regulated CaMKIIdelta alternative splicing temporally reprograms excitation-contraction coupling in cardiac muscle. Cell 120(1):59-72PubMed: 15652482 MGI: J:96039

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Genetics

Srsf1^tm1Xdfu

Allele Symbol: Srsf1^tm1Xdfu

Allele Name	targeted mutation 1, Xiang-Dong Fu
Allele Type	Targeted (Conditional ready (e.g. floxed), No functional change)
Allele Synonym(s)	ASF/SF2^II; Sfrs1^fl
Gene Symbol and Name	Srsf1, serine/arginine-rich splicing factor 1
Gene Synonym(s)
Strain of Origin	129S4/SvJae
Chromosome	11
Molecular Note	A loxP site was inserted upstream of exon 2 and a floxed neo-tk cassette was inserted downstream of exon 4. The neo-tk cassette was removed by transient expression of cre recombinase in correctly targeted ES cells. Resulting mutant animals carry loxP-flanked exons 2-4.

Disease/Phenotype

Disease Terms

Research Areas By Phenotype

This mouse can be used to support research in many areas including:

Research Tools
- Cre-lox System
  - loxP-flanked Sequences
- Cardiovascular Research
  - Cre-lox System

Mammalian Phenotype Terms by Genotype

The following phenotype information is associated with a similar, but not exact match to this JAX^® Mice strain

Genotype: Srsf1^tm1Xdfu/Srsf1^tm1Xdfu Tg(Chx10-EGFP/cre,-ALPP)2Clc/0
involves: 129S4/SvJae * C57BL/6 * SJL

cellular phenotype

increased retinal apoptosis
- at E12.5, 13.5, and 18.0, cell death is significantly elevated; increased apoptosis is observed primarily in central retina at E12.5 and 13.5 while periphery is affected at E18.0

nervous system phenotype

abnormal retinal horizontal cell morphology
- by P7, cell number is significantly reduced

decreased amacrine cell number
- by P7, cell number is significantly reduced
- 2-fold decrease in BrdU-labeled cells is observed at P7 (BrdU injection at E16)

decreased retinal ganglion cell number
- by P7, few ganglion cells are detected in retina

abnormal optic nerve morphology
- thin optic nerve

vision/eye phenotype

abnormal retina inner limiting membrane morphology
- inner limiting membrane (ILM) structure is perturbed; retina has dysmorphic fibrous layer instead of well-organized ILM

abnormal retina morphology
- size of retina is decreased compared to wild-type
- retina is detached from retinal pigment epithelium (RPE), while abutting the lens
- eyes analyzed at P7 following BrdU injection at E16 show reduced cell numbers in outer nuclear layer (ONL), inner nuclear layer (INL), and ganglion cell layer (GCL)

abnormal retinal ganglion layer morphology
- ganglion cell layer (GCL) exhibits decreased cellularity in postnatal mice

abnormal optic nerve morphology
- thin optic nerve

decreased retinal ganglion cell number
- by P7, few ganglion cells are detected in retina

increased retinal apoptosis
- at E12.5, 13.5, and 18.0, cell death is significantly elevated; increased apoptosis is observed primarily in central retina at E12.5 and 13.5 while periphery is affected at E18.0

retinal degeneration
- by P14, rosettes are observed in retina
- almost complete retinal degeneration has occurred by P30; entire eye is absent in mutants
- ciliary body is fused to retina, so that it could not be separated from neural retina

abnormal retinal horizontal cell morphology
- by P7, cell number is significantly reduced

microphthalmia
- newborn mice have small eyes
- total weight of eyes is 50% of wild-type

decreased amacrine cell number
- 2-fold decrease in BrdU-labeled cells is observed at P7 (BrdU injection at E16)
- by P7, cell number is significantly reduced

References

Xu X; Yang D; Ding JH; Wang W; Chu PH; Dalton ND; Wang HY; Bermingham JR Jr; Ye Z; Liu F; Rosenfeld MG; Manley JL; Ross J Jr; Chen J; Xiao RP; Cheng H; Fu XD. 2005. ASF/SF2-regulated CaMKIIdelta alternative splicing temporally reprograms excitation-contraction coupling in cardiac muscle. Cell 120(1):59-72PubMed: 15652482 MGI: J:96039

Additional - Srsf1^tm1Xdfu related

Technical Support

CONTACT TECHNICAL SUPPORT

Genotyping Protocols
- Standard PCR:Srsf1
- Genotyping resources and troubleshooting
Breeding Considerations

Homozygous or heterozygous mice may be bred to maintain a colony.
- Additional Breeding and Husbandry Support
Citation
When using the B6;129S4-Srsf1^tm1Xdfu/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #018020 in your Materials and Methods section.

Animal Health Reports

Facility Barrier Level Descriptions

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200

Pricing & Availability

Cryo Recovery

Domestic
International

Live Mouse

Age

Genotype

Price

weeks

Cryorecovery - Pricing

Service/Product	Description	Price
	Heterozygous or wildtype for Srsf1<tm1Xdfu>

Related Products and Services

Frozen Mouse Embryo	B6;129S4-Srsf1<tm1Xdfu>/J	$2595.00
Frozen Mouse Embryo	B6;129S4-Srsf1<tm1Xdfu>/J	$2595.00
Frozen Mouse Embryo	B6;129S4-Srsf1<tm1Xdfu>/J	$3373.50
Frozen Mouse Embryo	B6;129S4-Srsf1<tm1Xdfu>/J	$3373.50

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Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.

The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.

Terms Of Use

General Terms and Conditions

Questions about Terms of Use

Additional Use Restrictions Apply

Use of MICE by companies or for-profit entities requires a license prior to shipping.

Licensing Information

Phone: 207-288-6470

Email: TechTran@jax.org

Donating Investigator

Genetic overview

Research Applications

Base Price

Detailed Description

Development

Control Suggestions

Approximate Controls

Additional Information

Selected References

Srsf1tm1Xdfu

Allele Symbol: Srsf1tm1Xdfu

Disease Terms

Research Areas By Phenotype

This mouse can be used to support research in many areas including:

Mammalian Phenotype Terms by Genotype

Genotype: Srsf1tm1Xdfu/Srsf1tm1Xdfu Tg(Chx10-EGFP/cre,-ALPP)2Clc/0involves: 129S4/SvJae * C57BL/6 * SJL

cellular phenotype

nervous system phenotype

vision/eye phenotype

References

Additional - Srsf1tm1Xdfu related

Genotyping Protocols

Breeding Considerations

Citation

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200

Live Mouse

Cryorecovery - Pricing

Related Products and Services

Payment Terms and Conditions

The Jackson Laboratory's Genotype Promise

Terms Of Use

Additional Use Restrictions Apply

Licensing Information

Srsf1^tm1Xdfu

Allele Symbol: Srsf1^tm1Xdfu

Genotype: Srsf1^tm1Xdfu/Srsf1^tm1Xdfu Tg(Chx10-EGFP/cre,-ALPP)2Clc/0
involves: 129S4/SvJae * C57BL/6 * SJL

Additional - Srsf1^tm1Xdfu related