These floxed mutant mice possess loxP sites flanking exons 2-4 of the Srsf1 gene. This strain may be useful for generating conditional mutations in applications related to studies of embryonic retinal development.
Xiang-Dong Fu, University of California, San Diego
Srsf1 (serine/arginine-rich splicing factor 1) is a serine/arginine (SR)-rich protein associated with the regulation of consititive splicing.
These targeted mutation mice carry a floxed allele of the gene. When crossed with a cre strain, tissue specific excision of exons 2-4 can be accomplished.
Ubiquitous excision of the floxed segment is early embryonic lethal. Conditional knockout in cardiomyocytes with a Myl2-cre strain causes postnatal contraction defects and dilated cardiomyopathy.
When bred to mice carrying Tg(Chx10-EGFP/cre,-ALPP)2Clc (see Stock No. 005105 for example) Cre recombinase expression in retina and Muller glial cells, this mutant mouse strain may be useful in studies of embryonic retinal development.
A loxP site was placed in intron 1 and a loxP-flanked neomycin resistance cassette was introduced to intron 4 using 129S4/SvJae-derived J1 embryonic stem (ES)cells. Positive ES cell clones were transfected with a cre-expression plasmid to excise the floxed neomycin cassette. The strain was backcrossed to C57BL/6J one time by the donating laboratory.
|Allele Name||targeted mutation 1, Xiang-Dong Fu|
|Allele Type||Targeted (Conditional ready (e.g. floxed), No functional change)|
|Allele Synonym(s)||ASF/SF2II; Sfrs1fl|
|Gene Symbol and Name||Srsf1, serine/arginine-rich splicing factor 1|
|Strain of Origin||129S4/SvJae|
|Molecular Note||A loxP site was inserted upstream of exon 2 and a floxed neo-tk cassette was inserted downstream of exon 4. The neo-tk cassette was removed by transient expression of cre recombinase in correctly targeted ES cells. Resulting mutant animals carry loxP-flanked exons 2-4.|
Homozygous or heterozygous mice may be bred to maintain a colony.
When using the B6;129S4-Srsf1tm1Xdfu/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #018020 in your Materials and Methods section.