JAX Mouse Strain Datasheet - 018020

B6;129S4-Srsf1^tm1Xdfu/J

Stock No:: 018020

Targeted Mutation

Cryo Recovery

Overview

These floxed mutant mice possess loxP sites flanking exons 2-4 of the Srsf1 gene. This strain may be useful for generating conditional mutations in applications related to studies of embryonic retinal development.

Donating Investigator

Xiang-Dong Fu, University of California, San Diego

Genetic overview

Genetic Background	Generation

Srsf1^tm1Xdfu

Allele Type	Gene Symbol	Gene Name
Targeted (Conditional ready (e.g. floxed), No functional change)	Srsf1	serine/arginine-rich splicing factor 1

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Research Applications

Research Tools

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Base Price

Starting at:

$2,595.00 Domestic price Cryo Recovery

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Details

Detailed Description

Srsf1 (serine/arginine-rich splicing factor 1) is a serine/arginine (SR)-rich protein associated with the regulation of consititive splicing.

These targeted mutation mice carry a floxed allele of the gene. When crossed with a cre strain, tissue specific excision of exons 2-4 can be accomplished.

Ubiquitous excision of the floxed segment is early embryonic lethal. Conditional knockout in cardiomyocytes with a Myl2-cre strain causes postnatal contraction defects and dilated cardiomyopathy.

When bred to mice carrying Tg(Chx10-EGFP/cre,-ALPP)2Clc (see Stock No. 005105 for example) Cre recombinase expression in retina and Muller glial cells, this mutant mouse strain may be useful in studies of embryonic retinal development.

Development

A loxP site was placed in intron 1 and a loxP-flanked neomycin resistance cassette was introduced to intron 4 using 129S4/SvJae-derived J1 embryonic stem (ES)cells. Positive ES cell clones were transfected with a cre-expression plasmid to excise the floxed neomycin cassette. The strain was backcrossed to C57BL/6J one time by the donating laboratory.

Control Suggestions

Approximate Controls

000664 C57BL/6J

Additional Information

Considerations for Choosing Controls

Selected References

Xu X; Yang D; Ding JH; Wang W; Chu PH; Dalton ND; Wang HY; Bermingham JR Jr; Ye Z; Liu F; Rosenfeld MG; Manley JL; Ross J Jr; Chen J; Xiao RP; Cheng H; Fu XD. 2005. ASF/SF2-regulated CaMKIIdelta alternative splicing temporally reprograms excitation-contraction coupling in cardiac muscle. Cell 120(1):59-72. PubMed: 15652482 MGI: J:96039

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Genetics

Srsf1^tm1Xdfu

Allele Symbol: Srsf1^tm1Xdfu

Allele Name	targeted mutation 1, Xiang-Dong Fu
Allele Type	Targeted (Conditional ready (e.g. floxed), No functional change)
Allele Synonym(s)	ASF/SF2^II; Sfrs1^fl
Gene Symbol and Name	Srsf1, serine/arginine-rich splicing factor 1
Gene Synonym(s)	1110054N12Rik; 1110054N12Rik; 5730507C05Rik; 5730507C05Rik; 6330415C05Rik; 6330415C05Rik; AI482334; ASF; AW491331; RIKEN cDNA 1110054N12 gene; RIKEN cDNA 5730507C05 gene; RIKEN cDNA 6330415C05 gene; SF2; SF2p33; SFRS1; SRp30a; Sfrs1; Sfrs1; expressed sequence AI482334; expressed sequence AW491331; splicing factor, arginine/serine-rich 1 (ASF/SF2)
Strain of Origin	129S4/SvJae
Chromosome	11
Molecular Note	A loxP site was inserted upstream of exon 2 and a floxed neo-tk cassette was inserted downstream of exon 4. The neo-tk cassette was removed by transient expression of cre recombinase in correctly targeted ES cells. Resulting mutant animals carry loxP-flanked exons 2-4.

Disease/Phenotype

Disease Terms

Research Areas By Genotype

This mouse can be used to support research in many areas including:

Research Tools
- Cardiovascular Research
  - Cre-lox System
- Cre-lox System
  - loxP-flanked Sequences

Mammalian Phenotype Terms by Genotype

The following phenotype relates to a compound genotype created using this strain

Genotype: Srsf1^tm1Xdfu/Srsf1^tm1Xdfu Tg(Chx10-EGFP/cre,-ALPP)2Clc/0
involves: 129S4/SvJae * C57BL/6 * SJL

vision/eye phenotype

abnormal horizontal cell morphology
- by P7, cell number is significantly reduced

abnormal optic nerve morphology
- thin optic nerve

abnormal retina inner limiting membrane morphology
- inner limiting membrane (ILM) structure is perturbed; retina has dysmorphic fibrous layer instead of well-organized ILM

abnormal retina morphology
- eyes analyzed at P7 following BrdU injection at E16 show reduced cell numbers in outer nuclear layer (ONL), inner nuclear layer (INL), and ganglion cell layer (GCL)
- retina is detached from retinal pigment epithelium (RPE), while abutting the lens
- size of retina is decreased compared to wild-type

abnormal retinal ganglion layer morphology
- ganglion cell layer (GCL) exhibits decreased cellularity in postnatal mice

decreased amacrine cell number
- 2-fold decrease in BrdU-labeled cells is observed at P7 (BrdU injection at E16)
- by P7, cell number is significantly reduced

decreased retinal ganglion cell number
- by P7, few ganglion cells are detected in retina

increased retinal apoptosis
- at E12.5, 13.5, and 18.0, cell death is significantly elevated; increased apoptosis is observed primarily in central retina at E12.5 and 13.5 while periphery is affected at E18.0

microphthalmia
- newborn mice have small eyes
- total weight of eyes is 50% of wild-type

retinal degeneration
- almost complete retinal degeneration has occurred by P30; entire eye is absent in mutants
- by P14, rosettes are observed in retina
- ciliary body is fused to retina, so that it could not be separated from neural retina

nervous system phenotype

abnormal horizontal cell morphology
- by P7, cell number is significantly reduced

abnormal optic nerve morphology
- thin optic nerve

decreased amacrine cell number
- 2-fold decrease in BrdU-labeled cells is observed at P7 (BrdU injection at E16)
- by P7, cell number is significantly reduced

decreased retinal ganglion cell number
- by P7, few ganglion cells are detected in retina

cellular phenotype

increased retinal apoptosis
- at E12.5, 13.5, and 18.0, cell death is significantly elevated; increased apoptosis is observed primarily in central retina at E12.5 and 13.5 while periphery is affected at E18.0

References

Xu X; Yang D; Ding JH; Wang W; Chu PH; Dalton ND; Wang HY; Bermingham JR Jr; Ye Z; Liu F; Rosenfeld MG; Manley JL; Ross J Jr; Chen J; Xiao RP; Cheng H; Fu XD. 2005. ASF/SF2-regulated CaMKIIdelta alternative splicing temporally reprograms excitation-contraction coupling in cardiac muscle. Cell 120(1):59-72. PubMed: 15652482 MGI: J:96039

Additional - Srsf1^tm1Xdfu related

Cheng Y; Luo C; Wu W; Xie Z; Fu X; Feng Y. 2016. Liver-Specific Deletion of SRSF2 Caused Acute Liver Failure and Early Death in Mice. Mol Cell Biol 36(11):1628-38. PubMed: 27022105 MGI: J:236188
Kanadia RN; Clark VE; Punzo C; Trimarchi JM; Cepko CL. 2008. Temporal requirement of the alternative-splicing factor Sfrs1 for the survival of retinal neurons. Development 135(23):3923-33. PubMed: 18987029 MGI: J:143205

Pricing & Availability

Cryo Recovery

Domestic
International

Live Mouse

Age

Genotype

Price

weeks

Cryorecovery - Pricing

Service	Genotype	Price
	Heterozygous or wildtype for Srsf1<tm1Xdfu>

Progeny testing is not required

The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 10 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks.

The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation.

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Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.

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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.

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General Terms and Conditions

Questions about Terms of Use

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Licensing Information

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Donating Investigator

Genetic overview

Research Applications

Base Price

Detailed Description

Development

Control Suggestions

Approximate Controls

Additional Information

Selected References

Srsf1tm1Xdfu

Allele Symbol: Srsf1tm1Xdfu

Disease Terms

Research Areas By Genotype

This mouse can be used to support research in many areas including:

Mammalian Phenotype Terms by Genotype

Genotype: Srsf1tm1Xdfu/Srsf1tm1Xdfu Tg(Chx10-EGFP/cre,-ALPP)2Clc/0involves: 129S4/SvJae * C57BL/6 * SJL

vision/eye phenotype

nervous system phenotype

cellular phenotype

References

Additional - Srsf1tm1Xdfu related

Genotyping Protocols

Breeding Considerations

Citation

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200

Live Mouse

Cryorecovery - Pricing

Progeny testing is not required

Payment Terms and Conditions

The Jackson Laboratory's Genotype Promise

Terms of Use

Additional Use Restrictions Apply

Licensing Information

JAX® Mice, Products & Services Conditions of Use

No Warranty

Credit for PRODUCTS or SERVICES

Animal Care and Use for SERVICES

No Liability

Srsf1^tm1Xdfu

Allele Symbol: Srsf1^tm1Xdfu

Genotype: Srsf1^tm1Xdfu/Srsf1^tm1Xdfu Tg(Chx10-EGFP/cre,-ALPP)2Clc/0
involves: 129S4/SvJae * C57BL/6 * SJL

Additional - Srsf1^tm1Xdfu related