These floxed mutant mice possess loxP sites flanking exons 2-4 of the Srsf1 gene. This strain may be useful for generating conditional mutations in applications related to studies of embryonic retinal development.
Xiang-Dong Fu, University of California, San Diego
Genetic Background | Generation |
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|
Allele Type | Gene Symbol | Gene Name |
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Targeted (Conditional ready (e.g. floxed), No functional change) | Srsf1 | serine/arginine-rich splicing factor 1 |
Srsf1 (serine/arginine-rich splicing factor 1) is a serine/arginine (SR)-rich protein associated with the regulation of consititive splicing.
These targeted mutation mice carry a floxed allele of the gene. When crossed with a cre strain, tissue specific excision of exons 2-4 can be accomplished.
Ubiquitous excision of the floxed segment is early embryonic lethal. Conditional knockout in cardiomyocytes with a Myl2-cre strain causes postnatal contraction defects and dilated cardiomyopathy.
When bred to mice carrying Tg(Chx10-EGFP/cre,-ALPP)2Clc (see Stock No. 005105 for example) Cre recombinase expression in retina and Muller glial cells, this mutant mouse strain may be useful in studies of embryonic retinal development.
A loxP site was placed in intron 1 and a loxP-flanked neomycin resistance cassette was introduced to intron 4 using 129S4/SvJae-derived J1 embryonic stem (ES)cells. Positive ES cell clones were transfected with a cre-expression plasmid to excise the floxed neomycin cassette. The strain was backcrossed to C57BL/6J one time by the donating laboratory.
Allele Name | targeted mutation 1, Xiang-Dong Fu |
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Allele Type | Targeted (Conditional ready (e.g. floxed), No functional change) |
Allele Synonym(s) | ASF/SF2II; Sfrs1fl |
Gene Symbol and Name | Srsf1, serine/arginine-rich splicing factor 1 |
Gene Synonym(s) | |
Strain of Origin | 129S4/SvJae |
Chromosome | 11 |
Molecular Note | A loxP site was inserted upstream of exon 2 and a floxed neo-tk cassette was inserted downstream of exon 4. The neo-tk cassette was removed by transient expression of cre recombinase in correctly targeted ES cells. Resulting mutant animals carry loxP-flanked exons 2-4. |
Homozygous or heterozygous mice may be bred to maintain a colony.
When using the B6;129S4-Srsf1tm1Xdfu/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #018020 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous or wildtype for Srsf1<tm1Xdfu> |
Frozen Mouse Embryo | B6;129S4-Srsf1<tm1Xdfu>/J | $2595.00 |
Frozen Mouse Embryo | B6;129S4-Srsf1<tm1Xdfu>/J | $2595.00 |
Frozen Mouse Embryo | B6;129S4-Srsf1<tm1Xdfu>/J | $3373.50 |
Frozen Mouse Embryo | B6;129S4-Srsf1<tm1Xdfu>/J | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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