B6;129S4-Srsf2^tm1Xdfu/J

Stock number: 018019
Research areas: Research Tools

Description

These mutant Srsf2 mice possess loxP sites flanking exons 1-2. When crossed with a cre strain, the floxed sequence is excised in cre-expressing tissues of the offspring.

Detailed description

Srsf2 (serine/arginine-rich splicing factor 2) is a serine/arginine (SR)-rich protein associated with the regulation of consititive splicing.

These targeted mutation mice carry a floxed allele of the gene. When crossed with a cre strain, tissue specific excision of the first two coding exons can be accomplished.

Ubiquitous excision of the floxed segment is embryonic lethal. Conditional knockout in thymus using an Lck-cre strain causes a defect in T cell maturation by altering alternative splicing of CD45. Disruption of expression in the heart with an Myl2-cre strain causes dilated cardiomyopathy.

Development

A loxP site was placed 5' of the first coding exon and a loxP-flanked neomycin resistance cassette was introduced 3' of the second coding exon. The mutation was created in 129S4/SvJae-derived J1 embryonic stem (ES)cells. Positive ES cell clones were transfected with a cre-expression plasmid to excise the floxed neomycin cassette. This strain was backcrossed two times to C57BL/6J by the donating laboratory.

Allele

Symbol: Srsf2^tm1Xdfu
Name: targeted mutation 1, Xiang Dong Fu
MGI accession ID: MGI:3036846
Generation method: Targeted
Attributes: Conditional ready (e.g. floxed); No functional change
Marker symbol: Srsf2
Marker name: serine/arginine-rich splicing factor 2
Chromosome: 11
Strain of origin: 129S4/SvJae
Molecular note: LoxP sites flanking exons 1 and 2 were introduced to the gene by homologous recombination. The floxed region includes the entire coding region. The targeted allele was verified by Southern blot analysis.