These mutant Srsf2 mice possess loxP sites flanking exons 1-2. When crossed with a cre strain, the floxed sequence is excised in cre-expressing tissues of the offspring.
Xiang-Dong Fu, University of California, San Diego
Genetic Background | Generation |
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|
Allele Type | Gene Symbol | Gene Name |
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Targeted (Conditional ready (e.g. floxed), No functional change) | Srsf2 | serine/arginine-rich splicing factor 2 |
Srsf2 (serine/arginine-rich splicing factor 2) is a serine/arginine (SR)-rich protein associated with the regulation of consititive splicing.
These targeted mutation mice carry a floxed allele of the gene. When crossed with a cre strain, tissue specific excision of the first two coding exons can be accomplished.
Ubiquitous excision of the floxed segment is embryonic lethal. Conditional knockout in thymus using an Lck-cre strain causes a defect in T cell maturation by altering alternative splicing of CD45. Disruption of expression in the heart with an Myl2-cre strain causes dilated cardiomyopathy.
A loxP site was placed 5' of the first coding exon and a loxP-flanked neomycin resistance cassette was introduced 3' of the second coding exon. The mutation was created in 129S4/SvJae-derived J1 embryonic stem (ES)cells. Positive ES cell clones were transfected with a cre-expression plasmid to excise the floxed neomycin cassette. This strain was backcrossed two times to C57BL/6J by the donating laboratory.
Allele Name | targeted mutation 1, Xiang Dong Fu |
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Allele Type | Targeted (Conditional ready (e.g. floxed), No functional change) |
Allele Synonym(s) | SC35flox; Srsf2fl |
Gene Symbol and Name | Srsf2, serine/arginine-rich splicing factor 2 |
Gene Synonym(s) | |
Strain of Origin | 129S4/SvJae |
Chromosome | 11 |
Molecular Note | LoxP sites flanking exons 1 and 2 were introduced to the gene by homologous recombination. The floxed region includes the entire coding region. The targeted allele was verified by Southern blot analysis. |
Mutations Made By | Xiang-Dong Fu, University of California, San Diego |
Homozygotes or heterozygotes may be bred to maintain a colony.
When using the B6;129S4-Srsf2tm1Xdfu/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #018019 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous for Srsf2<tm1Xdfu> |
Frozen Mouse Embryo | B6;129S4-Srsf2<tm1Xdfu>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6;129S4-Srsf2<tm1Xdfu>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6;129S4-Srsf2<tm1Xdfu>/J Frozen Embryo | $3373.50 |
Frozen Mouse Embryo | B6;129S4-Srsf2<tm1Xdfu>/J Frozen Embryo | $3373.50 |
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