STOCK Fkbp4^tm1Dvds/J

Stock number: 017990
Research areas: Reproductive Biology Research

Description

These Fkbp4 (or FKBP52) knockout mice are viable as homozygotes, but not fertile. They may have applications in studies related to androgen, progesterone, and glucocorticoid receptor signaling pathways and reproduction.

Detailed description

FKBP4 (or FKBP52) is a member of the immunophilin protein family and is known to play a role in protein folding and trafficking. FKBP4 functions as a co-chaperone that binds to HSP90 in steroid receptor complexes. In this strain the targeted allele lacks the entire Fkbp4 coding region. Homozygous mice are viable, but are not fertile. Male mice exhibit mild to severe hypospadia, ambiguous external genitalia, malformed seminal vesicles and a small-to-absent anterior prostate gland. Spermatozoa have reduced motility and fertilization efficiency. The donating investigator reports that homozygous females are infertile due to implantation failure. This strain may be useful for studying androgen, progesterone, and glucocorticoid receptor signaling pathways and reproduction.

Development

A targeting vector was designed to exchange a neomycin selection cassette for the entire coding region (exons 1-10, approximately 9 kb) of the Fkbp4 (FKBP52) gene. The construct was electroporated into 129X1/SvJ derived embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6 blastocysts and resulting chimeric mice were bred C57BL/6 mice. The strain was maintained at the Mayo Clinic and then transferred to the laboratory of Dr. Sudhansu Dey. The Fkbp4 allele was introgressed into an outbred CD-1 background and has been maintained on the CD-1 (Crl:CD1) background for many generations. Upon arrival, mice were bred to C57BL/6J inbred mice (Stock No. 000664) for at least one generation to establish the colony.

Allele

Symbol: Fkbp4^tm1Dvds
Name: targeted mutation 1, David F Smith
MGI accession ID: MGI:3579432
Generation method: Targeted
Attributes: Null/Knockout
Marker symbol: Fkbp4
Marker name: FK506 binding protein 4
Chromosome: 6
Strain of origin: 129X1/SvJ
Molecular note: A neomycin resistance gene replaced the entire coding sequence. Western blot failed to detect protein in thymic cytosol of mutants.