ULK1 is an evolutionarily conserved serine threonine kinase which plays a role in the regulation of neurotransmitter trafficking and axonal growth. ULK1 is a homolog of yeast autophagy-related gene (Atg1), which has been implicated in organelle autophagy during cell growth and survival.
These Ulk1FL mutant mice possess a loxP site upstream of exon 1, and a loxP-flanked neomycin resistance (neo) cassette downstream of exon 3 of the Unc-51 like kinase 1 (Ulk1) gene. Mice that are homozygous for this allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When these mutant mice are bred to mice that express Cre recombinase, resulting offspring will have exons 1-3, and the neo cassette, deleted in cre-expressing tissues. These ULK1-deficient mice display a slight defect in autophagy during reticulocyte maturation. When bred to B6;129-Ulk2tm1Thsn/J mice (Stock No. 017977), and subsequently to mice that express Cre recombinase, double KO mice die within 24 hours of birth. When mouse embryonic fibroblasts (MEFs) are cultured from these double KO mice amino-acid deprivation does not induce autophagy, while normal autophagy is evident during glucose deprivation and ammonia induction.
A targeting vector was designed to insert a loxP site upstream of exon 1, and a loxP-flanked neomycin resistance (neo) cassette downstream of exon 3 of the Unc-51 like kinase 1 (Ulk1) gene. The construct was electroporated into (129X1/SvJ x 129S1/Sv)F1-Kitl+-derived R1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6 blastocysts and resulting chimeric males were bred with C57BL/6J females. These mice were backcrossed to C57BL/6J mice for at least 10 generations. Upon arrival, mice were bred to C57BL/6J inbred mice (Stock No. 000664) for at least one generation to establish the colony.
|Allele Name||targeted mutation 1, Craig B Thompson|
|Allele Type||Targeted (Conditional ready (e.g. floxed), No functional change)|
|Allele Synonym(s)||Ulk1FL; Ulk1tm1Kund|
|Gene Symbol and Name||Ulk1, unc-51 like kinase 1|
|Strain of Origin||(129X1/SvJ x 129S1/Sv)F1-Kitl+|
|Molecular Note||A loxP site was targeted upstream of exon 1 and a floxed neomycin cassette was placed downstream of exon 3. Southern blotting confirmed correct targeting of the locus.|
|Mutations Made By|| |
Craig Thompson, Memorial Sloan Kettering Cancer Center
When maintaining a live colony, homozygous mice may be bred together.
When using the Ulk1FL mouse strain in a publication, please cite the originating article(s) and include JAX stock #017976 in your Materials and Methods section.