These Rosa26-LSL-Rac1b mice allow for the conditional expression of a Rac1 isoform b cDNA. They may be useful in applications associated with the initiation and progression of tumorigenesis.
Joseph L Kissil, The Scripps Research Institute, Scripps Florida
Genetic Background | Generation |
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Allele Type | Gene Symbol | Gene Name |
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Targeted (Conditional ready (e.g. floxed), Inserted expressed sequence, Humanized sequence) | Gt(ROSA)26Sor | gene trap ROSA 26, Philippe Soriano |
The Rosa26-LSL-Rac1b allele contains a loxP-flanked neo-STOP cassette upstream of human RAS-related C3 botulinum substrate 1 (Rac1) isoform b cDNA inserted into the Gt(ROSA)26Sor locus. The Rac proteins regulate signaling pathways that control the shape, motility and growth of cells. Mice homozygous for the Rosa26-LSL-Rac1b allele are viable and fertile. Rac1b is a GTP-bound, active Rac1 splice variant which has been found to be upregulated in colon and breast cancer cells. In the absence of Cre, RAC1 expression is prevented by the floxed STOP sequence. After removal of the loxP-flanked STOP cassette via cre-mediated recombination, RAC1 expression is evident in the cre-expressing tissues of the offspring. For example, when these mice receive an intranasal instillation of a cre-expressing adenovirus, the removal of the STOP cassette in the lung results in RAC1 expression. These inoculated mice show no overt phenotype. When bred to B6.129S4-Krastm4Tyj/J mice (Stock No. 008179), expressing a cre-inducible oncogenic K-ras allele, intranasal instillation of a cre-expressing adenovirus results in the initiation and progression of lung tumors with an increase in the tumor volume to lung volume ratio above that of the oncogenic K-ras allele alone.
Human RAS-related C3 botulinum substrate 1 (Rac1) isoform b cDNA was inserted into a Gt(ROSA)26Sor vector, downstream of the loxP-flanked cassette, which contains a neomycin selectable marker and a transcriptional stop sequence. This Rosa26-LSL-Rac1b construct was electroporated into 129Sv/J-derived embryonic stem (ES) cells. The resulting offspring were bred to C57BL/6 mice to establish the colony. This mutant strain was maintained on a mixed C57BL/6 x 129 background. Upon arrival at The Jackson Laboratory, mutant mice were bred to C57BL/6J inbred mice (Stock No. 000664) for at least one generation to establish the colony.
Expressed Gene | RAC1, Rac family small GTPase 1, human |
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Site of Expression |
Allele Name | targeted mutation 1, Joseph L Kissil |
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Allele Type | Targeted (Conditional ready (e.g. floxed), Inserted expressed sequence, Humanized sequence) |
Allele Synonym(s) | Rosa26-LSL-Rac1b |
Gene Symbol and Name | Gt(ROSA)26Sor, gene trap ROSA 26, Philippe Soriano |
Gene Synonym(s) | |
Expressed Gene | RAC1, Rac family small GTPase 1, human |
Strain of Origin | 129 |
Chromosome | 6 |
Molecular Note | Human RAS-related C3 botulinum substrate 1 (Rac1) isoform b cDNA was inserted into a Gt(ROSA)26Sorvector, downstream of the loxP-flanked cassette, which contains a neomycin selectable marker and a transcriptional stop sequence. |
When maintaining a live colony, homozygous mice may be bred together.
When using the Rosa26-LSL-Rac1b mouse strain in a publication, please cite the originating article(s) and include JAX stock #017962 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous or wildtype for Gt(ROSA)26Sor<tm1(RAC1*)Jkis> |
Frozen Mouse Embryo | B6;129-Gt(ROSA)26Sor<tm1(RAC1*)Jkis>/J | $2595.00 |
Frozen Mouse Embryo | B6;129-Gt(ROSA)26Sor<tm1(RAC1*)Jkis>/J | $2595.00 |
Frozen Mouse Embryo | B6;129-Gt(ROSA)26Sor<tm1(RAC1*)Jkis>/J | $3373.50 |
Frozen Mouse Embryo | B6;129-Gt(ROSA)26Sor<tm1(RAC1*)Jkis>/J | $3373.50 |
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