These transgenic mice express a tamoxifen-inducible cre recombinase from the human KRT18, keratin 18, promoter. When induced, cre activity is observed in Merkel cells of the skin and simple epithelia. They may be useful in lineage tracing.
Cedric Blanpain, Universite Libre de Bruxelles
Genetic Background | Generation |
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Allele Type |
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Transgenic (Recombinase-expressing, Inducible) |
Keratin 18 is an intermediate filament cytoskeleton protein found in adult simple epithelial tissues.
Merkel cells are neuroendocrine cells found in touch domes (mechanoreceptor zones in the skin) of the epithelial basal layer and some mucosa. Merkel cells express both intermediate filaments and neuropeptides.
These transgenic mice express CreERT2 under the direction of the human KRT18, keratin 18, promoter.
When these K18-CREER#23 mice are bred with mice containing a loxP-flanked sequence, tamoxifen-inducible, Cre-mediated recombination will result in deletion of the flanked sequences in Merkel cells and simple epithelia. When bred to ROSA-YFP reporter mice, pulse/chase adminstration of tamoxifen results in the fluorescent labeling of 17% of vibrissa Merkel cells 1 week after pulse, and 9% after 3 weeks. Mice that are hemizygous for the targeted mutation are viable, normal in size and do not display any gross physical or behavioral abnormalities. The Donating Investigator has not attempted to make this strain homozygous.
A transgenic construct containing Cre-ERT2 under the control of the human KRT18, keratin 18, promoter, SV40 intron and SV40 polyadenylation site sequence was injected into fertilized C57BL/6 mouse eggs. Founder line 23 was subsequently established. The transgenic animals were then bred to CD1 mice. Upon arrival at The Jackson Laboratory, the mice were crossed to C57BL/6J (Stock No. 000664) at least once to establish the colony.
Expressed Gene | cre/ERT2, Cre recombinase and estrogen receptor 1 (human) fusion gene, |
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Site of Expression | When mice carrying this transgene are bred with mice containing a loxP-flanked sequence, tamoxifen-inducible, Cre-mediated recombination will result in deletion of the flanked sequences in Merkel cells and simple epithelia. |
Allele Name | transgene insertion 23, Cedric Blanpain |
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Allele Type | Transgenic (Recombinase-expressing, Inducible) |
Allele Synonym(s) | K18-CREER |
Gene Symbol and Name | Tg(KRT18-cre/ERT2)23Blpn, transgene insertion 23, Cedric Blanpain |
Gene Synonym(s) | |
Promoter | KRT18, keratin 18, human |
Expressed Gene | cre/ERT2, Cre recombinase and estrogen receptor 1 (human) fusion gene, |
Site of Expression | When mice carrying this transgene are bred with mice containing a loxP-flanked sequence, tamoxifen-inducible, Cre-mediated recombination will result in deletion of the flanked sequences in Merkel cells and simple epithelia. |
Strain of Origin | C57BL/6 |
Chromosome | UN |
Molecular Note | A fragment encoding cre recombinase fused to a modified estrogen receptor ligand-binding domain (ERT2) was cloned into a vector containing the human KRT18 promoter. The construct was inected into fertilized oocytes to generated transgenic mice. Two lines were obtained. Line 23 was used in the original reference. |
Mutations Made By | Cedric Blanpain, Universite Libre de Bruxelles |
When maintaining a live colony, hemizygous mice may be bred together, to wildtype siblings, or to C57BL/6J inbred mice (Stock No. 000664). The Donating Investigator has not attempted to make this strain homozygous.
When using the K18-CREER #23 mouse strain in a publication, please cite the originating article(s) and include JAX stock #017948 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
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Hemizygous or Non Carrier for Tg(KRT18-cre/ERT2)23Blpn |
Frozen Mouse Embryo | STOCK Tg(KRT18-cre/ERT2)23Blpn/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | STOCK Tg(KRT18-cre/ERT2)23Blpn/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | STOCK Tg(KRT18-cre/ERT2)23Blpn/J Frozen Embryo | $3373.50 |
Frozen Mouse Embryo | STOCK Tg(KRT18-cre/ERT2)23Blpn/J Frozen Embryo | $3373.50 |
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