B6.Cg-Lair1^tm1Jco/J

Stock number: 017905
Research areas: Research Tools

Description

These mutant mice possess loxP sites flanking exons 4 to 8 of the leukocyte-associated Ig-like receptor 1 (Lair1) gene, an inhibitory receptor protein, and may be useful for studying immune cell regulation.

Detailed description

LAIR1, an inhibitory receptor protein with a high affinity for collagen, is expressed on most cells of the immune system and is associated with inhibition of the cytotoxic activity of NK and CD8+ T cells, BCR-induced B cell activation and proliferation, and CD3 signaling and cytokine production by T cells. These mutant mice possess loxP sites flanking exons 4 to 8 of the (Lair1) gene. Mice that are homozygous for this allele are viable, fertile, and normal in size. When bred to mice that express Cre recombinase, resulting offspring will have exons 4-8 deleted in the cre-expressing tissues. This strain may be useful for studying immune cell regulation.

Please note: this strain may be segregating the Thy1^a allele.

Development

A targeting vector was designed to insert a loxP site upstream of exon 4 followed by a frt-flanked neomycin resistance (neo) cassette and a second loxP site downstream of exon 8 of the Lair1 gene. Exons 4-8 contain two immunoreceptor tyrosine-based inhibition motifs (ITIM)that interact with the tyrosine phosphatases SHP1, SHP2 and SRC kinase. This construct was electroporated into B6.Cg-Thy1^a-derived Bruce4 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6 blastocysts and resulting chimeric mice were bred to C57BL/6 mice.

Allele

Symbol: Lair1^tm1Jco
Name: targeted mutation 1, John E Coligan
MGI accession ID: MGI:5308462
Generation method: Targeted
Attributes: Conditional ready (e.g. floxed); No functional change
Synonyms: LAIR-1^fl/fl
Marker symbol: Lair1
Marker name: leukocyte-associated Ig-like receptor 1
Marker synonyms: 5133400O11Rik; CD305; CD306; D7Bwg0421e; Lair-1; mLair-1
Chromosome: 7
Strain of origin: B6.Cg-Thy1^a
Molecular note: A targeting vector was designed to insert a loxP site upstream of exon 4 followed by a frt-flanked neomycin resistance (neo) cassette and a second loxP site downstream of exon 8 via homologous recombination. Exons 4-8 contain two immunoreceptor tyrosine-based inhibition motifs (ITIM)that interact with the tyrosine phosphatases SHP1, SHP2 and SRC kinase.