These mutant mice possess loxP sites flanking exons 4 to 8 of the leukocyte-associated Ig-like receptor 1 (Lair1) gene, an inhibitory receptor protein, and may be useful for studying immune cell regulation.
John E Coligan, NIAID/NIH
Genetic Background | Generation |
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|
Allele Type | Gene Symbol | Gene Name |
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Targeted (Conditional ready (e.g. floxed), No functional change) | Lair1 | leukocyte-associated Ig-like receptor 1 |
LAIR1, an inhibitory receptor protein with a high affinity for collagen, is expressed on most cells of the immune system and is associated with inhibition of the cytotoxic activity of NK and CD8+ T cells, BCR-induced B cell activation and proliferation, and CD3 signaling and cytokine production by T cells. These mutant mice possess loxP sites flanking exons 4 to 8 of the (Lair1) gene. Mice that are homozygous for this allele are viable, fertile, and normal in size. When bred to mice that express Cre recombinase, resulting offspring will have exons 4-8 deleted in the cre-expressing tissues. This strain may be useful for studying immune cell regulation.
Please note: this strain may be segregating the Thy1a allele.
A targeting vector was designed to insert a loxP site upstream of exon 4 followed by a frt-flanked neomycin resistance (neo) cassette and a second loxP site downstream of exon 8 of the Lair1 gene. Exons 4-8 contain two immunoreceptor tyrosine-based inhibition motifs (ITIM)that interact with the tyrosine phosphatases SHP1, SHP2 and SRC kinase. This construct was electroporated into B6.Cg-Thy1a-derived Bruce4 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6 blastocysts and resulting chimeric mice were bred to C57BL/6 mice.
Allele Name | targeted mutation 1, John E Coligan |
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Allele Type | Targeted (Conditional ready (e.g. floxed), No functional change) |
Allele Synonym(s) | LAIR-1fl/fl |
Gene Symbol and Name | Lair1, leukocyte-associated Ig-like receptor 1 |
Gene Synonym(s) | |
Strain of Origin | B6.Cg-Thy1a |
Chromosome | 7 |
Molecular Note | A targeting vector was designed to insert a loxP site upstream of exon 4 followed by a frt-flanked neomycin resistance (neo) cassette and a second loxP site downstream of exon 8 via homologous recombination. Exons 4-8 contain two immunoreceptor tyrosine-based inhibition motifs (ITIM)that interact with the tyrosine phosphatases SHP1, SHP2 and SRC kinase. |
Mutations Made By | John Coligan, NIAID/NIH |
While maintaining a live colony, these mice are bred as homozygotes
When using the B6.Cg-Lair1tm1Jco/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #017905 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous or wildtype for Lair1<tm1Jco> |
Frozen Mouse Embryo | B6(Cg)-Lair1<tm1Jco>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6(Cg)-Lair1<tm1Jco>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6(Cg)-Lair1<tm1Jco>/J Frozen Embryo | $3373.50 |
Frozen Mouse Embryo | B6(Cg)-Lair1<tm1Jco>/J Frozen Embryo | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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