These mice possess loxP sites flanking exon 1 of the glutaminase (Gls) gene and have applications in studies of cellular energy metabolism.
Stephen Rayport, Columbia University and NYSPI
These mice possess loxP sites on either side of the Gls exon 1, bracketing the start codon. Glutaminase is involved in glutamate neurotransmitter recycling, nitrogen metabolism, glycolysis in the presence of oxygen (Warburg effect), and regulation of reactive oxygen species. Mice that are homozygous for this allele are viable, fertile and normal in size. When bred to mice that express Cre recombinase, the resulting offspring will have exon 1 and the start codon deleted in the cre-expressing tissue(s).
When used in conjunction with a Cre recombinase-expressing strain, this mutant mouse strain may be useful in studies related to glutamate neurotransmitter recycling, metabolic compensation in the kidney and anaerobic cancer cell metabolism.
A targeting vector was designed to insert a loxP site 16 bp upstream of the start codon in exon 1 and a loxP-flanked neomycin cassette in intron 1. This construct was electroporated into 129S1/Sv-Oca2+ Tyr+ Kitl+ derived W9.5 embryonic stem (ES) cells. Correctly targeted ES cells were injected into recipient blastocysts. The resulting chimeric animals were crossed to wildtype C57BL/6 mice. Progeny were then crossed to mice carrying Tg(EIIa-cre)C5379Lmgd to remove the neomycin cassette and leave exon 1 floxed. Mice then were bred to 129S6/SvEvTac for one generation. Upon arrival at The Jackson Laboratory, the mice were crossed to C57BL/6J (Stock No. 000664) at least once to establish the colony.
|Allele Name||targeted mutation 2.1, Stephen Rayport|
|Allele Type||Targeted (Conditional ready (e.g. floxed), No functional change)|
|Allele Synonym(s)||floxGLS1; GLS1lox|
|Gene Symbol and Name||Gls, glutaminase|
|Strain of Origin||129S1/Sv-Oca2+ Tyr+ Kitl+|
|Molecular Note||The targeting vector was designed to insert a loxP site 16 bp before the start codon in exon 1 and a loxP-flanked neomycin cassette in intron 1. Cre-mediated recombination removed the floxed neo cassette leaving exon 1 floxed.|
|Mutations Made By|| |
Stephen Rayport, Columbia University and NYSPI
When maintaining a live colony, these mice can be bred as homozygotes.
When using the floxGLS1 , GLS1lox mouse strain in a publication, please cite the originating article(s) and include JAX stock #017894 in your Materials and Methods section.
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