These transgenic mice overexpress mouse Diap3, diaphanous homolog 3 (Drosophila), in the cochlea, exhibit progressive hearing loss and may be useful in studies of progressive hearing loss and auditory neuropathy.
Marci M Lesperance, University of Michigan
Genetic Background | Generation |
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Allele Type |
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Transgenic (Inserted expressed sequence) |
Diaphanous homolog 3 is a member of the diaphanous-related formin protein family which are involved in actin regulation and microtubule stabilization. A mutation in human DIAPH3 is a cause of autosomal dominant auditory neuropathy. These transgenic mice overexpress the mouse Diap3, diaphanous homolog 3 (Drosophila), gene under the control of the CAG, CMV enhancer/chicken beta-actin promoter, with approximately 1000-fold overexpression of Diap3 in the cochlea, as determined by qRT-PCR. Hemizygotes develop hearing loss between 8 and 16 weeks of age, which progresses to severe hearing loss by 24 weeks of age.
Hemizygotes have fewer inner hair cell ribbon synapses and disorganized inner hair cell stereocilia. Some transgenic mice die prematurely due to cardiac abnormalities (enlarged hearts, cardiomyopathy). Mice hemizygous for the transgenic insert are viable and fertile. The Donating Investigator has not attempted to make the strain homozygous.
A transgenic construct containing the mouse Diap3, diaphanous homolog 3 (Drosophila), gene under the control of the CAG, CMV enhancer/chicken beta-actin promoter, was injected into fertilized FVB/NJ mouse eggs. Founder line 924 was subsequently established. The mice were maintained on the FVB/NJ background. Upon arrival at The Jackson Laboratory, the mice were crossed to FVB/NJ (Stock No. 001800) at least once to establish the colony.
Expressed Gene | Diaph3, diaphanous related formin 3, mouse, laboratory |
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Site of Expression |
Allele Name | transgene insertion 924, Marci Lesperance |
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Allele Type | Transgenic (Inserted expressed sequence) |
Allele Synonym(s) | |
Gene Symbol and Name | Tg(CAG-Diap3)924Lesp, transgene insertion 924, Marci Lesperance |
Gene Synonym(s) | |
Promoter | ACTB, actin, beta, chicken |
Expressed Gene | Diaph3, diaphanous related formin 3, mouse, laboratory |
Strain of Origin | FVB/NJ |
Chromosome | UN |
Molecular Note | A transgenic construct contains the mouse gene under the control of the CAG, CMV enhancer/chickenbeta-actin, promoter. Founder line 924 was subsequently established. |
When maintaining a live colony, hemizygous mice may be bred together, to wildtype siblings, or to FVB/NJ inbred mice (Stock No. 001800). The Donating Investigator has not attempted to make the strain homozygous.
When using the FVB/NJ-Tg(CAG-Diap3)924Lesp/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #017881 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
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Hemizygous or Non carrier or Tg(CAG-Diap3)924Lesp |
Frozen Mouse Embryo | FVB/NJ-Tg(CAG-Diap3)924Lesp/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | FVB/NJ-Tg(CAG-Diap3)924Lesp/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | FVB/NJ-Tg(CAG-Diap3)924Lesp/J Frozen Embryo | $3373.50 |
Frozen Mouse Embryo | FVB/NJ-Tg(CAG-Diap3)924Lesp/J Frozen Embryo | $3373.50 |
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