An EGFP reporter disrupts expression of the Kitl gene in this targeted mutation strain. EGFP is primarily expressed by perivascular endothelial and stromal cells throughout the bone marrow in heterozygotes.
Sean J Morrison, University of Texas Southwestern Medical Center
Genetic Background | Generation |
---|---|
|
Allele Type | Gene Symbol | Gene Name |
---|---|---|
Targeted (Reporter, Null/Knockout) | Kitl | kit ligand |
An Enhanced Green Fluorescent Protein (EGFP) reporter disrupts expression of Kitl (kit ligand; also called stem cell factor, Scf) in this targeted mutation strain. EGFP is primarily expressed by perivascular endothelial and stromal cells throughout the bone marrow. Homozygotes die perinatally with severe anemia. Kitl transcripts are nearly undetectable by quantitatve RT-PCR in the livers of newborn homozygotes. Overall cellularity of the newborn liver is reduced fivefold in homozygotes and approximately twofold in heterozygotes. Hematopoietic stem cell numbers are reduced about eightfold in the livers of newborn homozygotes as compared with wildtype and heterozygous controls. Heterozygotes have a diluted coat color and often have a white spot on the abdomen.
An EGFP-polyA-Frt-Neo-Frt cassette was introduced to the second exon of the targeted gene via 129S6/SvEvTac-derived W4 embryonic stem (ES) cells. Chimeric mice were bred with C57BL/Ka mice to obtain germline transmission, then bred with C57BL/Ka background mice expressing Flp to excise the neomycin cassette. This strain was backcrossed for 10 generations to C57BL/Ka by the donating laboratory. Upon arrival at The Jackson Laboratory, C57BL/6J was used for rederivation.
Expressed Gene | GFP, Green Fluorescent Protein, |
---|---|
Site of Expression | EGFP is primarily expressed by perivascular endothelial and stromal cells throughout the bone marrow. |
Allele Name | targeted mutation 1.1, Sean J Morrison |
---|---|
Allele Type | Targeted (Reporter, Null/Knockout) |
Allele Synonym(s) | Scfgfp |
Gene Symbol and Name | Kitl, kit ligand |
Gene Synonym(s) | |
Expressed Gene | GFP, Green Fluorescent Protein, |
Site of Expression | EGFP is primarily expressed by perivascular endothelial and stromal cells throughout the bone marrow. |
Strain of Origin | 129S6/SvEvTac |
Chromosome | 10 |
Molecular Note | A targeting vector was designed to replace exon 2 with EGFP (in frame) and an FRT-flanked neomycin resistance cassette. The neomycin resistance cassette was removed by flp-mediated recombination. qRT-PCR confirmed the absence of transcript expression in the liver. |
Mutations Made By | Sean Morrison, University of Texas Southwestern Medical Center |
Heterozygous mice are viable and fertile. Homozygotes die perinatally with severe anemia.
When using the Scf (Kitl) knockin mouse strain in a publication, please cite the originating article(s) and include JAX stock #017860 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous or wildtype for Kitl<tm1.1Sjm |
Frozen Mouse Embryo | STOCK Kitl<tm1.1Sjm>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | STOCK Kitl<tm1.1Sjm>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | STOCK Kitl<tm1.1Sjm>/J Frozen Embryo | $3373.50 |
Frozen Mouse Embryo | STOCK Kitl<tm1.1Sjm>/J Frozen Embryo | $3373.50 |
Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
What information were you hoping to find through your search?
How easy was it to find what you were looking for?
We may wish to follow up with you. Enter your email if you are happy for us to connect and reachout to you with more questions.
Please Enter a Valid Email Address
Thank you for sharing your feedback! We are working on improving the JAX Mice search. Come back soon for exciting changes.