These Csf3r knockout mice are useful for studying granulopoiesis and hematopoietic stem cell regulation.
Daniel C. Link, Washington University Medical School
A neo cassette replaces exons 3-8 of the endogenous colony stimulating factor 3 receptor (Csf3r) gene, abolishing gene function. Homozygotes are viable and fertile. CSF3R is the transmembrane receptor for colony stimulating factor 3 (G-CSF), a cytokine that controls the production, differentiation, and function of granulocytes. Expressed in precursor cells in the bone marrow, CSF3R also initiates the maturation of granulocytes. These mice contain a decreased number of circulating neutrophils. They also exhibit a 50% decrease of mature myeloid cells, metamyelocytes, and hematopoietic stem cells, and granulocytes in bone marrow. Neutrophils from these mice display impaired chemotaxis in response to G-CSF and Interleukin 8 (Il8). Increased apoptosis is evident in cultured bone marrow derived mononuclear cells.
A targeting vector was designed to replace exons 3-8 encoding the colony stimulating factor 3 receptor (Csf3r) gene with a neomycin resistance (neo) cassette. The construct was electroporated into 129X1/SvJ-derived RW-4 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6 blastocysts and the resulting chimeric males were bred to Swiss Black females. These mice were backcrossed for at least 10 generations on to a C57BL/6J background. Upon arrival at The Jackson Laboratory, mice were bred to C57BL/6J (Stock No. 000664) for at least one generation to establish the colony.
|Allele Name||targeted mutation 1, Daniel C Link|
|Allele Type||Targeted (Null/Knockout)|
|Gene Symbol and Name||Csf3r, colony stimulating factor 3 receptor (granulocyte)|
|Strain of Origin||129X1/SvJ|
|Molecular Note||A neomycin resistance cassette replaced exons 3 - 8, which include the translational initiation site, signal peptide, and the ligand binding domain.|
|Mutations Made By|| |
Daniel Link, Washington University Medical School
When maintaining a live colony, homozygous mice may be bred together. Homozygous mice are produced at a reduced Mendelian ratio (~10%) from heterozygous matings.
When using the G-CSFR- mouse strain in a publication, please cite the originating article(s) and include JAX stock #017838 in your Materials and Methods section.
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