B6.129X1(Cg)-Csf3r^tm1Link/J

Stock number: 017838
Product name: G-CSFR-
Research areas: Apoptosis Research, Cell Biology Research, Immunology, Inflammation and Autoimmunity Research

Description

These Csf3r knockout mice are useful for studying granulopoiesis and hematopoietic stem cell regulation.

Detailed description

A neo cassette replaces exons 3-8 of the endogenous colony stimulating factor 3 receptor (Csf3r) gene, abolishing gene function. Homozygotes are viable and fertile. CSF3R is the transmembrane receptor for colony stimulating factor 3 (G-CSF), a cytokine that controls the production, differentiation, and function of granulocytes. Expressed in precursor cells in the bone marrow, CSF3R also initiates the maturation of granulocytes. These mice contain a decreased number of circulating neutrophils. They also exhibit a 50% decrease of mature myeloid cells, metamyelocytes, and hematopoietic stem cells, and granulocytes in bone marrow. Neutrophils from these mice display impaired chemotaxis in response to G-CSF and Interleukin 8 (Il8). Increased apoptosis is evident in cultured bone marrow derived mononuclear cells.

Development

A targeting vector was designed to replace exons 3-8 encoding the colony stimulating factor 3 receptor (Csf3r) gene with a neomycin resistance (neo) cassette. The construct was electroporated into 129X1/SvJ-derived RW-4 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6 blastocysts and the resulting chimeric males were bred to Swiss Black females. These mice were backcrossed for at least 10 generations on to a C57BL/6J background. Upon arrival at The Jackson Laboratory, mice were bred to C57BL/6J (Stock No. 000664) for at least one generation to establish the colony.

Allele

Symbol: Csf3r^tm1Link
Name: targeted mutation 1, Daniel C Link
MGI accession ID: MGI:2156982
Generation method: Targeted
Attributes: Null/Knockout
Marker symbol: Csf3r
Marker name: colony stimulating factor 3 receptor (granulocyte)
Chromosome: 4
Strain of origin: 129X1/SvJ
Molecular note: A neomycin resistance cassette replaced exons 3 - 8, which include the translational initiation site, signal peptide, and the ligand binding domain.