LGB-Cre (originally BLG-cre) transgenic mice have the beta-lactoglobulin promoter directing Cre recombinase expression to mammary gland during lactation. This strain may be useful for studying basal-like cancer and breast cancer, as well as providing a useful tool for testing new therapeutics.
IMR Colony, The Jackson Laboratory
Genetic Background | Generation |
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Allele Type |
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Transgenic (Recombinase-expressing) |
Mice hemizygous for the BLG-Cre transgene are viable and fertile, with the beta-lactoglobulin (BLG) promoter directing Cre recombinase expression to mammary gland during lactation. When these BLG-Cre transgenic mice are bred with mice containing loxP-flanked sequences, Cre-mediated recombination will result in deletion of the floxed sequences in cre-expressing tissues in the offspring.
Of note, the donating investigator of the triple mutant BLG-Cre; Brca1F22-24/F22-24; p53+/- mice (Stock No. 012620) reports that mice should be allowed to go through two rounds of pregnancy and then set aside to allow Cre activation and the loss of floxed gene function.
The BLG-Cre transgene was designed by Dr. Alan R. Clarke (while at University Medical School, Edinburgh, United Kingdom) to have Cre recombinase under the control of the sheep mammary gland specific promoter of the ovine beta-lactoglobulin (BLG) gene. The construct included exon 1 of sheep BLG, and a Cre recombinase with a modified initiation codon and a polyadenylation signal (polyA). The construct was microinjected into the pronuclei of (CBA x C57BL/6) fertilized oocytes, and mice from founder line 74 were identified and bred to C57BL/6 mice. These mice were intercrossed to establish a colony of BLG-cre mice on a mixed background.
BLG-Cre transgenic mice were bred to C57BL/6;129S7-Trp53tm1Brd and C57BL/6;129-Brca1tm1Aash mice, and the resulting triple mutant mice were sent to The Jackson Laboratory Repository as Stock No. 012620. Upon arrival, some mice were selectively bred with wildtype mice from the colony or with C57BL/6J inbred mice (Stock No. 000664) to generate this single mutant colony harboring only the BLG-Cre transgene (as Stock No. 017836).
Expressed Gene | cre, cre recombinase, bacteriophage P1 |
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Site of Expression | Cre recombinase is active in the mammary gland. |
Allele Name | transgene insertion 74, Alan R Clarke |
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Allele Type | Transgenic (Recombinase-expressing) |
Allele Synonym(s) | BLG-Cre; Blg-CreTg |
Gene Symbol and Name | Tg(LGB-cre)74Acl, transgene insertion 74, Alan R Clarke |
Gene Synonym(s) | |
Promoter | LGB, beta-lactoglobulin, sheep |
Expressed Gene | cre, cre recombinase, bacteriophage P1 |
Site of Expression | Cre recombinase is active in the mammary gland. |
Strain of Origin | (CBA x C57BL/6) |
Chromosome | UN |
Molecular Note | This transgene expresses Cre recombinase under the control of a sheep beta-lactoglobulin promoter (LGB), active in the mammary gland. |
Mutations Made By | Alan Clarke, University Medical School, Edinburgh, Un |
When maintaining a live colony, mice hemizygous for the BLG-cre transgene may be bred with wildtype (noncarrier) mice from the colony.
Of note for Cre recombinase applications, the donating investigator of the triple mutant BLG-Cre; Brca1F22-24/F22-24; p53+/- mice (Stock No. 012620) reports that mice should be allowed to go through two rounds of pregnancy and then set aside to allow Cre activation and the loss of floxed gene function.
When using the STOCK Tg(LGB-cre)74Acl/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #017836 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
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Hemizygous or Non Carrier for Tg(LGB-cre)74Acl |
Frozen Mouse Embryo | STOCK Tg(LGB-cre)74Acl/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | STOCK Tg(LGB-cre)74Acl/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | STOCK Tg(LGB-cre)74Acl/J Frozen Embryo | $3373.50 |
Frozen Mouse Embryo | STOCK Tg(LGB-cre)74Acl/J Frozen Embryo | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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