STOCK Tg(LGB-cre)74Acl/J

Stock number: 017836
Research areas: Cancer Research, Research Tools

Description

LGB-Cre (originally BLG-cre) transgenic mice have the beta-lactoglobulin promoter directing Cre recombinase expression to mammary gland during lactation. This strain may be useful for studying basal-like cancer and breast cancer, as well as providing a useful tool for testing new therapeutics.

Detailed description

Mice hemizygous for the BLG-Cre transgene are viable and fertile, with the beta-lactoglobulin (BLG) promoter directing Cre recombinase expression to mammary gland during lactation. When these BLG-Cre transgenic mice are bred with mice containing loxP-flanked sequences, Cre-mediated recombination will result in deletion of the floxed sequences in cre-expressing tissues in the offspring.

Of note, the donating investigator of the triple mutant BLG-Cre; Brca1^{F22-24/F22-24}; p53^+/- mice (Stock No. 012620) reports that mice should be allowed to go through two rounds of pregnancy and then set aside to allow Cre activation and the loss of floxed gene function.

Development

The BLG-Cre transgene was designed by Dr. Alan R. Clarke (while at University Medical School, Edinburgh, United Kingdom) to have Cre recombinase under the control of the sheep mammary gland specific promoter of the ovine beta-lactoglobulin (BLG) gene. The construct included exon 1 of sheep BLG, and a Cre recombinase with a modified initiation codon and a polyadenylation signal (polyA). The construct was microinjected into the pronuclei of (CBA x C57BL/6) fertilized oocytes, and mice from founder line 74 were identified and bred to C57BL/6 mice. These mice were intercrossed to establish a colony of BLG-cre mice on a mixed background.

BLG-Cre transgenic mice were bred to C57BL/6;129S7-Trp53^tm1Brd and C57BL/6;129-Brca1^tm1Aash mice, and the resulting triple mutant mice were sent to The Jackson Laboratory Repository as Stock No. 012620. Upon arrival, some mice were selectively bred with wildtype mice from the colony or with C57BL/6J inbred mice (Stock No. 000664) to generate this single mutant colony harboring only the BLG-Cre transgene (as Stock No. 017836).

Allele

Symbol: Tg(LGB-cre)74Acl
Name: transgene insertion 74, Alan R Clarke
MGI accession ID: MGI:2137694
Generation method: Transgenic
Attributes: Recombinase-expressing
Marker symbol: Tg(LGB-cre)74Acl
Marker name: transgene insertion 74, Alan R Clarke
Chromosome: UN
Strain of origin: (CBA x C57BL/6)
Expressed genes: cre,cre recombinase,bacteriophage P1
Site of expression: Cre recombinase is active in the mammary gland.
Molecular note: This transgene expresses Cre recombinase under the control of a sheep beta-lactoglobulin promoter (LGB), active in the mammary gland.