Overview

Also Known As:Brca1^F22-24

The Brca1^F22-24 allele has loxP sites flanking exons 22-24 of the Brca1 gene. These mice may be useful in generating tissue-specific BRCA1 deletions for studying basal-like cancer and breast cancer.

Donating Investigator

IMR Colony, The Jackson Laboratory

Genetic overview

Genetic Background	Generation

Brca1^tm1Aash

Allele Type	Gene Symbol	Gene Name
Targeted (Conditional ready (e.g. floxed), No functional change)	Brca1	breast cancer 1, early onset

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Research Applications

Research Tools
Cancer Research
Endocrine Deficiency Research

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Base Price

Starting at:

$2,854.50 Domestic price Cryo Recovery

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Details

Detailed Description

The Brca1^{F22-24/F22-24} allele has loxP sites flanking exons 22-24 of the breast cancer 1 gene (Brca1). Homozygous Brca1^{F22-24/F22-24} mice are viable and fertile with no observed abnormalities. When bred to mice that express Cre recombinase, the resulting offspring will have the sequences encoding the second terminal BRCT domain deleted in the cre-expressing tissue(s). These Brca1^{F22-24/F22-24} mice may be useful in generating tissue-specific BRCA1 deletions for studying human basal-like cancer and breast cancer.

For example, when crossed to a strain expressing Cre recombinase in mammary gland during lactation (see Stock No. 017836), mice exhibit increased mammary gland tumor incidence.

Development

The Brca1^F22-24 targeted mutation was designed by Dr. Alan Ashworth (Breakthrough Breast Cancer Research Centre, London) to replace exons 22-24 of the mouse breast cancer 1 gene (Brca1) with a loxP site, a cDNA sequence encoded by exons 22-24, a 3' myc epitope, bovine growth hormone polyA signal, a second loxP site, a splice acceptor sequence, and a PGK-neo cassette. This construct was electroporated into (129X1/SvJ x 129S1/Sv)F1-Kitl⁺-derived R1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6J blastocysts and the resulting chimeric mice were bred to C57BL/6 mice. These mice were intercrossed to establish a colony of Brca1^{F22-24/F22-24} mice on a mixed background.

Brca1^F22-24 mice were bred to C57BL/6;129S7-Trp53^tm1Brd and C57BL/6;CBA-Tg(LGB-cre)74Acl mice, and the resulting triple mutant mice were sent to The Jackson Laboratory Repository as Stock No. 012620. Upon arrival, some mice were selectively bred with wildtype mice from the colony or with C57BL/6J inbred mice (Stock No. 000664) to generate this Brca1^F22-24 single mutant colony (as Stock No. 017835).

Control Suggestions

Approximate Controls

000664 C57BL/6J

Additional Information

Considerations for Choosing Controls

Selected References

McCarthy A; Savage K; Gabriel A; Naceur C; Reis-Filho JS; Ashworth A. 2007. A mouse model of basal-like breast carcinoma with metaplastic elements. J Pathol 211(4):389-98PubMed: 17212342 MGI: J:119996

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Genetics

Brca1^tm1Aash

Allele Symbol: Brca1^tm1Aash

Allele Name	targeted mutation 1, Alan Ashworth
Allele Type	Targeted (Conditional ready (e.g. floxed), No functional change)
Allele Synonym(s)	Brca1^F22-24
Gene Symbol and Name	Brca1, breast cancer 1, early onset
Gene Synonym(s)
Site of Expression	mammary epithelial cells
Strain of Origin	(129X1/SvJ x 129S1/Sv)F1-Kitl⁺
Chromosome	11
Molecular Note	Exons 22-24 were replaced with a floxed sequence containing cDNA for exons 22-24 with an in frame myc epitope and a bovine growth hormone poly A sequence. This vector allows conditional excision of the second, terminal BRCT domain. Downstream of the floxed sequence, a splice acceptor and PGK-neo were inserted.
Mutations Made By	Alan Ashworth, University of California, San Francisco

Disease/Phenotype

Disease Terms

Model with phenotypic similarity to human disease where etiologies involve orthologs. Human genes are associated with this disease. Orthologs of those genes appear in the mouse genotype(s).

breast cancer

Research Areas By Phenotype

This mouse can be used to support research in many areas including:

Research Tools
- Reproductive Biology Research
  - Cre-lox System
- Cre-lox System
  - loxP-flanked Sequences
- Genetics Research
  - Mutagenesis and Transgenesis: Cre-lox System
  - Mutagenesis and Transgenesis
- Developmental Biology Research
  - Cre-lox System
Cancer Research
- Cre-lox System
  - loxP-flanked Sequences
- Other
  - tumor metastasis
Endocrine Deficiency Research
- Mammary Gland Defects

Mammalian Phenotype Terms by Genotype

The following phenotype information is associated with a similar, but not exact match to this JAX^® Mice strain

Genotype: Brca1^tm1Aash/Brca1^tm1Aash
involves: 129S1/Sv * 129X1/SvJ * C57BL/6

normal phenotype

no abnormal phenotype detected
- Normal - mice have no overt phenotype and normal lifespans

Genotype: Brca1^tm1Aash/Brca1^tm1Aash Tg(LGB-cre)74Acl/0
involves: 129S1/Sv * 129X1/SvJ * C57BL/6 * CBA

digestive/alimentary phenotype

increased salivary adenocarcinoma incidence
- some mice (which have not gone through pregnancies) develop salivary gland myoepithelial tumors

endocrine/exocrine gland phenotype

increased salivary adenocarcinoma incidence
- some mice (which have not gone through pregnancies) develop salivary gland myoepithelial tumors

increased mammary gland tumor incidence
- mammary tumors occur in these control mice at lower frequency (12%: 5/43 animals) and much longer latency (12-15 months) compared to mice on a Trp43-haploinsufficient background

integument phenotype

increased mammary gland tumor incidence
- mammary tumors occur in these control mice at lower frequency (12%: 5/43 animals) and much longer latency (12-15 months) compared to mice on a Trp43-haploinsufficient background

neoplasm

increased salivary adenocarcinoma incidence
- some mice (which have not gone through pregnancies) develop salivary gland myoepithelial tumors

increased mammary gland tumor incidence
- mammary tumors occur in these control mice at lower frequency (12%: 5/43 animals) and much longer latency (12-15 months) compared to mice on a Trp43-haploinsufficient background

References

McCarthy A; Savage K; Gabriel A; Naceur C; Reis-Filho JS; Ashworth A. 2007. A mouse model of basal-like breast carcinoma with metaplastic elements. J Pathol 211(4):389-98PubMed: 17212342 MGI: J:119996

Additional - Brca1^tm1Aash related

Technical Support

CONTACT TECHNICAL SUPPORT

Genotyping Protocols
- Standard PCR:Brca1
- Standard PCR:Brca1
- Genotyping resources and troubleshooting
Breeding Considerations

When maintaining a live colony, mice homozygous for the Brca1^{F22-24/F22-24} allele may be bred together.
- Additional Breeding and Husbandry Support
Citation
When using the Brca1^F22-24 mouse strain in a publication, please cite the originating article(s) and include JAX stock #017835 in your Materials and Methods section.

Animal Health Reports

Facility Barrier Level Descriptions

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200

Pricing & Availability

Cryo Recovery

Domestic
International

Live Mouse

Age

Genotype

Price

weeks

Cryorecovery - Pricing

Service/Product	Description	Price
> >	Heterozygous for Brca1<tm1Aash>

Related Products and Services

Frozen Mouse Embryo	STOCK Brca1<tm1Aash>/J Frozen Embryo	$2595.00
Frozen Mouse Embryo	STOCK Brca1<tm1Aash>/J Frozen Embryo	$2595.00
Frozen Mouse Embryo	STOCK Brca1<tm1Aash>/J Frozen Embryo	$3373.50
Frozen Mouse Embryo	STOCK Brca1<tm1Aash>/J Frozen Embryo	$3373.50

Payment Terms and Conditions

Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.

The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.

Terms Of Use

General Terms and Conditions

Questions about Terms of Use

Licensing Information

Phone: 207-288-6470

Email: TechTran@jax.org

Also Known As:Brca1F22-24

Donating Investigator

Genetic overview

Research Applications

Base Price

Detailed Description

Development

Control Suggestions

Approximate Controls

Additional Information

Selected References

Brca1tm1Aash

Allele Symbol: Brca1tm1Aash

Disease Terms

Model with phenotypic similarity to human disease where etiologies involve orthologs. Human genes are associated with this disease. Orthologs of those genes appear in the mouse genotype(s).

Research Areas By Phenotype

This mouse can be used to support research in many areas including:

Mammalian Phenotype Terms by Genotype

Genotype: Brca1tm1Aash/Brca1tm1Aashinvolves: 129S1/Sv * 129X1/SvJ * C57BL/6

normal phenotype

Genotype: Brca1tm1Aash/Brca1tm1Aash Tg(LGB-cre)74Acl/0involves: 129S1/Sv * 129X1/SvJ * C57BL/6 * CBA

digestive/alimentary phenotype

endocrine/exocrine gland phenotype

integument phenotype

neoplasm

References

Additional - Brca1tm1Aash related

Genotyping Protocols

Breeding Considerations

Citation

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200

Live Mouse

Cryorecovery - Pricing

Related Products and Services

Payment Terms and Conditions

The Jackson Laboratory's Genotype Promise

Terms Of Use

Licensing Information

Also Known As:Brca1^F22-24

Brca1^tm1Aash

Allele Symbol: Brca1^tm1Aash

Genotype: Brca1^tm1Aash/Brca1^tm1Aash
involves: 129S1/Sv * 129X1/SvJ * C57BL/6

Genotype: Brca1^tm1Aash/Brca1^tm1Aash Tg(LGB-cre)74Acl/0
involves: 129S1/Sv * 129X1/SvJ * C57BL/6 * CBA

Additional - Brca1^tm1Aash related