These NSG-Tg(hu-mSCF) mice provide a supportive microenvironment that is conducive to engraftment of human hematopoietic stem cells and would be useful in studies of human mast cell development and allergic responses.
Dr. Leonard D. Shultz, The Jackson Laboratory
Genetic Background | Generation |
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|
Allele Type | Gene Symbol | Gene Name |
---|---|---|
Spontaneous | Prkdc | protein kinase, DNA activated, catalytic polypeptide |
Allele Type | Gene Symbol | Gene Name |
---|---|---|
Targeted (Null/Knockout) | Il2rg | interleukin 2 receptor, gamma chain |
Allele Type |
---|
Transgenic (Inserted expressed sequence, Humanized sequence) |
Due to demand, this colony is being expanded. An estimated distribution date is provided above.
These mice express a 220 amino acid isoform of the human membrane-bound stem cell factor (hSCF or KITLG) in combination with the severe combined immunodeficiency allele (scid) of the protein kinase, DNA activated, catalytic polypeptide (Prkdc) gene and the Il2rgtm1Wjl mutation of the interleukin 2 receptor, gamma chain gene. Newborn NSG-Tg(hu-mSCF) mice engraft human hematopoietic stem cells without pre-conditioning irradiation treatment and develop mature functional humanized immune systems, capable of rejecting histoincompatible human skin grafts. Differentiated c-Kit+ human mast cells can be detected in bone marrow, spleen, and mucosal lung, gastric, and intestinal tissues of engrafted mutant mice.
A transgenic construct containing the human stem cell factor (hSCF or KIT ligand, KITLG) cDNA encoding the 220 amino acid isoform under the control of the the human phosphoglycerate kinase 1 (PGK1) promoter, and splice donor/splice acceptor sequences, was microinjected into the pronuclei of fertilized C3H/HeJ eggs. Founder line 441 was subsequently established.
Mice hemizygous for the transgene were crossed to NOD.Cg-Prkdcscid mice (Stock No. 001303) for 10 generations. The mice were then crossed to NOD.Cg-Prkdcscid Il2rgtm1Wjl mice (Stock No. 005557), and bred to homozygosity for all the transgene/alleles (males are hemizygous for the X-linked Il2rgtm1Wjl targeted mutation).
Expressed Gene | KITLG, KIT ligand, human |
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Site of Expression |
Allele Name | severe combined immunodeficiency |
---|---|
Allele Type | Spontaneous |
Allele Synonym(s) | SCID |
Gene Symbol and Name | Prkdc, protein kinase, DNA activated, catalytic polypeptide |
Gene Synonym(s) | |
Site of Expression | T and B lymphocytes. |
Strain of Origin | C.BKa-Ighb/Icr |
Chromosome | 16 |
Molecular Note | A T-to-A transversion point mutation at a position corresponding to codon 4046 (codon 4095 in transcript ENSMUST00000023352.8) created a premature stop codon (p.Y4046*). |
Allele Name | targeted mutation 1, Warren J Leonard |
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Allele Type | Targeted (Null/Knockout) |
Allele Synonym(s) | [KO]gammac; CD132-; gammac-; gc-; Il2rgtm1Wjll; IL2Rgammanull |
Gene Symbol and Name | Il2rg, interleukin 2 receptor, gamma chain |
Gene Synonym(s) | |
Site of Expression | Primarily lymphoid cells. |
Strain of Origin | 129S4/SvJae |
Chromosome | X |
Molecular Note | A neomycin resistance cassette replaced part of exon 3 and all of exons 4 - 8 of the gene, resulting in the loss of most of the extracellular domain and all of the transmembrane and cytoplasmic domains of the protein. |
Mutations Made By | Dr. Warren Leonard, NHLBI, NIH |
Allele Name | transgene insertion 441, David A Williams |
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Allele Type | Transgenic (Inserted expressed sequence, Humanized sequence) |
Allele Synonym(s) | SCF220; Tg(huSCFh220)441Daw; TgN(PGKhSCF220) |
Gene Symbol and Name | Tg(PGK1-KITLG*220)441Daw, transgene insertion 441, David A Williams |
Gene Synonym(s) | |
Promoter | PGK1, phosphoglycerate kinase 1, human |
Expressed Gene | KITLG, KIT ligand, human |
Strain of Origin | C3H/HeJ |
Chromosome | UN |
Molecular Note | A 1.3 kb fragment containing a minigene with the human PGK1 promoter, splice donor/splice acceptor (SD/SA) sequence, a human stem cell factor (hSCF or KITLG) cDNA encoding the 220 amino acid isoform, and a polyA sequence was injected into the pronuclei of fertilized C3H/HeJ eggs. |
When maintaining a live colony, these mice can be bred homozygous for the Tg(PGK1-KITLG*220)441Daw transgene and the Prkdcscid spontaneous mutation, and homozygous (females) or hemizygous (males) for the Il2rgtm1Wjl targeted mutation.
When using the NSG-Tg(hu-mSCF) mouse strain in a publication, please cite the originating article(s) and include JAX stock #017830 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
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Homozygous for Prkdc<scid>, Homozygous females and Hemizygous males for Il2rg<tm1Wjl>, Homozygous for Tg(PGK1-KITLG*220)441Daw/ |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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