B6.Cg-Tg(tetO-Hamp)2181Nca/J

Stock number: 017791
Research areas: Metabolism Research, Research Tools

Description

This TRE.Hepc1 transgenic strain can be used to generate bitransgenic mice with spatio-temporal specific inducible overexpression of mouse Hamp, which may be useful in studies of anemia of inflammation and iron homeostasis.

Detailed description

Hepcidin antimicrobial peptide serves as the master regulator of iron homeostasis by limiting iron entry into the plasma from macrophages, intestinal enterocytes and other cells through binding to the iron-export protein ferroportin, and facilitating its internalization and degradation. Hepcidin antimicrobial peptide is induced by inflammation and is involved in anemia of chronic diseases and inflammation. These transgenic mice express mouse Hamp under control of the tetO, tetracycline-responsive regulatory element promoter. When crossed with a tTA strain, Hamp expression can be temporally regulated by doxycycline administration. For example, when TRE.Hepc1 mice are bred to a strain expressing tTA in liver, such as Stock No. 003563, the resulting double transgenic mice overexpress Hamp in the liver, resulting in hypoferremia, hypochromic and microcytic anemia, as well as anemia of inflammation. Doxycycline or tetracycline administration is expected to repress expression of the transgene in the double mutant mice.

Mice hemizygous for the TRE.Hepc1 transgene are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. The Donating Investigator reports that putative homozygotes are viable and fertile. The Donating Investigator notes that there can be a wide range of phenotypic expression in double mutant mice, which may be due to the tTA transgene used or regulation of the endogenous hepcidin.

Development

A transgenic construct containing a fragment of the mouse Hamp gene under the control of the tetO, tetracycline-responsive regulatory element promoter, and beta-globin poly-A tail signal sequence, was injected into FVB oocyte pronuclei. Founder line 2181 was subsequently established. The transgenic mice were bred to FVB, C57BL/6, BALB/c background mice and NMRI outbred mice. The mice were then backcrossed to C57BL/6 for 10 generations.

Allele

Symbol: Tg(tetO-Hamp)2181Nca
Name: transgene insertion 2181, Nancy C Andrews
MGI accession ID: MGI:5317183
Generation method: Transgenic
Attributes: Inducible; Inserted expressed sequence
Marker symbol: Tg(tetO-Hamp)2181Nca
Marker name: transgene insertion 2181, Nancy C Andrews
Chromosome: UN
Strain of origin: FVB
Expressed genes: Hamp,hepcidin antimicrobial peptide,mouse, laboratory
Molecular note: A transgenic construct contains a fragment of the mouse Hamp gene under the control of the tetO, tetracycline-responsive regulatory element promoter, with a beta-globin poly-A tail signal sequence. Founder line 2181 was established.