Overview

Also Known As: Prom1C-L

A CreER^T2 fusion protein and β-galactosidase (lacZ) gene knocked into the ATG start codon of Prom1 abolishes gene function in these mice. Useful applications include visualizing and tracking adult stem cell lineages.

Donating Investigator

Richard Gilbertson, St. Jude Children's Research Hospital

Genetic overview

Genetic Background	Generation
	N?+N1F20 (2019-11-01 00:00:00)

Prom1^{tm1(cre/ERT2)Gilb}

Allele Type	Gene Symbol	Gene Name
Targeted (Recombinase-expressing, Inducible)	Prom1	prominin 1

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Research Applications

Research Tools
Sensorineural Research
Internal/Organ Research

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Base Price

Starting at:

$278.00 Domestic price for female

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Details

Detailed Description

A CreER^T2 fusion protein, and an internal ribosome entry site (IRES)-β-galactosidase (lacZ) gene replace the ATG start codon of the prominin 1 (Prom1) gene, abolishing gene expression. PROM1 is a transmembrane glycoprotein expressed on the surface of stem cells. In this strain, endogenous Prom1 promoter/enhancer regions drive lacZ expression in embryonic central nervous system, kidney, intestine, and skeletal system. In adults, expression is seen in acinar and islet cells of the pancreas, goblet and columnar epithelial cells of the colon, photorepectoprs of the eye, renal tube cells, brain, lung, and both male and female reproductive systems. Cre-ER^T2 fusion gene activity is inducible and only observed following tamoxifen administration. When these Prom1^C-L mice are bred with mice containing loxP-flanked sequence, tamoxifen-inducible, Cre-mediated recombination will result in deletion of the floxed sequences in the Prom1-expressing cells of the offspring. For example when bred to Gt(ROSA)26Sor^tm1(EYFP)Cos/J mice (Stock No. 006148), tamoxifen induction results in cre expression in small intestinal crypt cells and the epithelial surface of the villi. Homozygotes are viable and fertile, but are blind due to retinal pigmentosa.

The Cre-ER^T2 fusion protein consists of Cre recombinase fused to a triple mutant form of the human estrogen receptor which does not bind its natural ligand (17β-estradiol) at physiological concentrations but will bind the synthetic estrogen receptor ligands 4-hydroxytamoxifen (OHT or tamoxifen) and, with lesser sensitivity, ICI 182780. Restricted to the cytoplasm, Cre-ER^T2 can only gain access to the nuclear compartment after exposure to tamoxifen. To counteract the mixed estrogen agonist effects of tamoxifen injections, which can result in late fetal abortions in pregnant mice, progesterone may be coadministered.

Development

A targeting vector was designed to replace the ATG start codon of the prominin 1 (Prom1) gene with, from 5' to 3', a CreER^T2 fusion protein, an internal ribosome entry site (IRES), nuclear localized β-galactosidase (lacZ), an SV40 polyadenylation signal, and a frt-flanked neomycin (neo) selection cassette. This construct was electroporated into 129S6/SvEv-derived CMTI-1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6 recipient blastocysts and chimeric males were bred with C57BL/6NCrl females. The resulting mice were bred to 129S4/SvJaeSor-Gt(ROSA)26Sor^tm1(FLP1)Dym/J mice (Stock No. 003946) to remove the neo cassette. These Prom1^C-L mice were backcrossed to C57BL/6NCrl for a few generations and then were maintained on a mixed B6;129S background.

Expression Data

Expressed Gene	cre/ERT2, Cre recombinase and estrogen receptor 1 (human) fusion gene,
Site of Expression	endogenous Prom1 promoter/enhancer regions drive lacZ expression in embryonic central nervous system, kidney, intestine, and skeletal system. In adults, expression is seen in acinar and islet cells of the pancreas, goblet and columnar epithelial cells of the colon, photorepectoprs of the eye, renal tube cells, brain, lung, and both male and female reproductive systems.

Control Suggestions

Approximate Controls

005304 C57BL/6NJ

Additional Information

Considerations for Choosing Controls

Selected References

Zhu L; Gibson P; Currle DS; Tong Y; Richardson RJ; Bayazitov IT; Poppleton H; Zakharenko S; Ellison DW; Gilbertson RJ. 2009. Prominin 1 marks intestinal stem cells that are susceptible to neoplastic transformation. Nature 457(7229):603-7PubMed: 19092805 MGI: J:144215

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Genetics

Prom1^{tm1(cre/ERT2)Gilb}

Allele Symbol: Prom1^{tm1(cre/ERT2)Gilb}

Allele Name	targeted mutation 1, Richard J Gilbertson
Allele Type	Targeted (Recombinase-expressing, Inducible)
Allele Synonym(s)	Prom1^{tm1(cre/ERT2)Gilb}; targeted mutation 1, Richard J Gilbertson
Gene Symbol and Name	Prom1, prominin 1
Gene Synonym(s)	CORD12; AC133; RIKEN cDNA 4932416E19 gene; Prom; PROML1; CD133; RP41; 4932416E19Rik; Prom-1; 4932416E19Rik; STGD4; MCDR2; MSTP061
Expressed Gene	cre/ERT2, Cre recombinase and estrogen receptor 1 (human) fusion gene,
Site of Expression	endogenous Prom1 promoter/enhancer regions drive lacZ expression in embryonic central nervous system, kidney, intestine, and skeletal system. In adults, expression is seen in acinar and islet cells of the pancreas, goblet and columnar epithelial cells of the colon, photorepectoprs of the eye, renal tube cells, brain, lung, and both male and female reproductive systems.
Strain of Origin	129S6/SvEvTac
Chromosome	5
Molecular Note	The ATG start site of the gene locus was replaced by a cassette containing a creERT2 fusion gene, an IRES-LacZ gene, and a FRT-flanked neomycin selection cassette. Correct targeting was confirmed by PCR. The neomycin selection cassette was later removed by crossing mice with Gt(ROSA)26Sor^tm1(FLP1)Dym mice. Beta-gal expression was detected in the neural tube, rib, kidney, and gut of E10.5-E16.5 heterozygote embryos. Beta-gal expression was also detected in numerous adult tissues including brain, kidney, retina, pancreas, and intestine. Cre expression was also detected in brain, kidney, lung, pancreas, and intestinal epithelium of heterozygote adult mice after tamoxifen administration. Inactivation of the endogenous gene locus was confirmed by RT-PCR of adult homozygous kidney.
Mutations Made By	Richard Gilbertson, St. Jude Children's Research Hospital

Disease/Phenotype

Disease Terms

Research Areas By Genotype

This mouse can be used to support research in many areas including:

Research Tools
- Genetics Research
  - Tissue/Cell Markers: neurons
  - Tissue/Cell Markers: chondrocytes
  - Tissue/Cell Markers: kidney specific marker
  - Tissue/Cell Markers: pancreatic beta cells
- Cre-lox System
  - Cre Recombinase Expression
  - Cre Recombinase Expression: Inducible
Sensorineural Research
- Retinal Degeneration
  - retinitis pigmentosa
Internal/Organ Research
- Skeleton
  - Bone

Mammalian Phenotype Terms by Genotype

The following phenotype information is associated with a similar, but not exact match to this JAX^® Mice strain

Genotype: Prom1^{tm1(cre/ERT2)Gilb}/Prom1^{tm1(cre/ERT2)Gilb}
involves: 129S6/SvEvTac

normal phenotype

no abnormal phenotype detected

References

Zhu L; Gibson P; Currle DS; Tong Y; Richardson RJ; Bayazitov IT; Poppleton H; Zakharenko S; Ellison DW; Gilbertson RJ. 2009. Prominin 1 marks intestinal stem cells that are susceptible to neoplastic transformation. Nature 457(7229):603-7PubMed: 19092805 MGI: J:144215

Additional - Prom1^{tm1(cre/ERT2)Gilb} related

Technical Support

Contact Technical Support

Genotyping Protocols
- Separated PCR:Prom1^{tm1(cre/ERT2)Gilb}
- Separated MCA:Prom1^{tm1(cre/ERT2)Gilb}
- Genotyping resources and troubleshooting
Breeding Considerations

When maintaining a live colony, homozygous mice may be bred together.
- Additional Breeding and Husbandry Support
Mating System
- Homozygote x Homozygote
Citation
When using the Prom1C-L mouse strain in a publication, please cite the originating article(s) and include JAX stock #017743 in your Materials and Methods section.

Animal Health Reports

Facility Barrier Level Descriptions

AX9 (Standard)

Pricing & Availability

Available

Domestic
International

Live Mouse

Age

Genotype

Price

weeks

Cryorecovery - Pricing

Service	Genotype	Price
	Heterozygous for Prom1<tm1(cre/ERT2)Gilb>

We will fulfill your order by providing at least two carriers for each strain ordered. The total number, sex, and genotypes provided will vary, although typically 8 or more animals are provided. Please check genotypes which will be recovered. While the genotypes of all animals produced will be communicated to you prior to scheduling shipment, the genotypes of animals provided may not reflect the mating scheme and genotypes described in the strain description. Animals are typically ready to ship in 11-14 weeks. If a second recovery is required to produce the minimum number of animals, then delivery time would increase to approximately 25 weeks. If we fail to produce animals of the correct genotype, you will not be charged. We cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation.

Related Products and Services

Frozen Mouse Embryo	B6N;129S-Prom1<tm1(cre/ERT2)Gilb>/J Frozen Embryo	$2595.00
Frozen Mouse Embryo	B6N;129S-Prom1<tm1(cre/ERT2)Gilb>/J Frozen Embryo	$2595.00
Frozen Mouse Embryo	B6N;129S-Prom1<tm1(cre/ERT2)Gilb>/J Frozen Embryo	$3373.50
Frozen Mouse Embryo	B6N;129S-Prom1<tm1(cre/ERT2)Gilb>/J Frozen Embryo	$3373.50

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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.

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General Terms and Conditions

Questions about Terms of Use

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Licensing Information

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Also Known As: Prom1C-L

Donating Investigator

Genetic overview

Research Applications

Base Price

Detailed Description

Development

Expression Data

Control Suggestions

Approximate Controls

Additional Information

Selected References

Prom1tm1(cre/ERT2)Gilb

Allele Symbol: Prom1tm1(cre/ERT2)Gilb

Disease Terms

Research Areas By Genotype

This mouse can be used to support research in many areas including:

Mammalian Phenotype Terms by Genotype

Genotype: Prom1tm1(cre/ERT2)Gilb/Prom1tm1(cre/ERT2)Gilbinvolves: 129S6/SvEvTac

normal phenotype

References

Additional - Prom1tm1(cre/ERT2)Gilb related

Genotyping Protocols

Breeding Considerations

Mating System

Citation

Animal Health Reports

Live Mouse

Cryorecovery - Pricing

Related Products and Services

Payment Terms and Conditions

The Jackson Laboratory's Genotype Promise

Terms of Use

Additional Use Restrictions Apply

Licensing Information

JAX® Mice, Products & Services Conditions of Use

No Warranty

Credit for PRODUCTS or SERVICES

Animal Care and Use for SERVICES

No Liability

Prom1^{tm1(cre/ERT2)Gilb}

Allele Symbol: Prom1^{tm1(cre/ERT2)Gilb}

Genotype: Prom1^{tm1(cre/ERT2)Gilb}/Prom1^{tm1(cre/ERT2)Gilb}
involves: 129S6/SvEvTac

Additional - Prom1^{tm1(cre/ERT2)Gilb} related