Mice harboring the Foxl1-Cre transgene express Cre recombinase under the control of the mouse Foxl1, forkhead box L1, promoter, in the mesenchymal cells of the stomach and intestine, and in oval cells in injured liver. They may be useful for generating conditional mutations in gastrointestinal mesenchyme and liver progenitor cells.
Klaus H. Kaestner, University of Pennsylvania
Genetic Background | Generation |
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Allele Type |
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Transgenic (Recombinase-expressing) |
These transgenic mice express Cre recombinase under the control of the mouse Foxl1, forkhead box L1, promoter. Foxl1 is expressed in the stomach and intestine, and in oval cells in injured liver tissue. Oval cells are bipotential progenitor cells that differentiate into cholangiocytes and hepatocytes. Cre recombinase expression is detected in the mesenchymal cells of the stomach and intestine, and in cells of the liver portal triad after injury, mimicking the endogenous Foxl1 expression pattern. Mice hemizygous for the transgenic insert are viable and fertile. The Donating Investigator reports that homozygotes are not viable.
The mouse bacterial artificial chromosome (BAC) RP23-446J14 containing the entire Foxl1 promoter and 189kb of flanking sequence, including the coding region, was modified in 2 steps. First, the Foxc2 coding region was replaced with an FRT site-flanked kanamycin cassette (to inactivate expression of Foxc2). Transient arabinose-inducible FLP recombinase expression was used to remove the kanamycin selection cassette. Next, the Foxl1 coding region was replaced with IRES-cre sequence. The modified BAC was then linearized and microinjected into (C57BL/6 X SJL)F1 fertilized oocytes. Founder animals were maintained on the B6;SJL background. Upon arrival at The Jackson Laboratory, the mice were crossed to C57BL/6J (Stock No. 000664) at least once to establish the colony.
Expressed Gene | cre, cre recombinase, bacteriophage P1 |
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Site of Expression | removal of kanamycin by flp-mediated recombination results in the expression of Cre in the mesenchymal cells of the stomach and intestines. |
Allele Name | transgene insertion 1, Klaus H Kaestner |
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Allele Type | Transgenic (Recombinase-expressing) |
Allele Synonym(s) | Foxl1-Cre |
Gene Symbol and Name | Tg(Foxl1-cre)1Khk, transgene insertion 1, Klaus H Kaestner |
Gene Synonym(s) | |
Promoter | Foxl1, forkhead box L1, mouse, laboratory |
Expressed Gene | cre, cre recombinase, bacteriophage P1 |
Site of Expression | removal of kanamycin by flp-mediated recombination results in the expression of Cre in the mesenchymal cells of the stomach and intestines. |
Strain of Origin | (C57BL/6 x SJL)F1 |
Chromosome | UN |
Molecular Note | Using BAC RP23-446J14, the researchers replaced the Foxl1 open reading frame with an IRES-cre cassette and the Foxc2 gene with an frt-flanked kanamycin cassette to prevent any expression from it. The kanamycin was later removed by flp-mediated recombination. Cre is expressed in the mesenchymal cells of the stomach and intestines. |
Mutations Made By | Klaus Kaestner, University of Pennsylvania |
When maintaining a live colony, hemizygous mice may be bred together, to wildtype siblings, or to C57BL/6J inbred mice (Stock No. 000664) or B6SJLF1/J (Stock No. 100012). The Donating Investigator reports that homozygotes are not viable.
When using the B6;SJL-Tg(Foxl1-cre)1Khk/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #017738 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
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Hemizygous or Non carrier for Tg(Foxl1-cre)1Khk |
Frozen Mouse Embryo | B6;SJL-Tg(Foxl1-cre)1Khk/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6;SJL-Tg(Foxl1-cre)1Khk/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6;SJL-Tg(Foxl1-cre)1Khk/J Frozen Embryo | $3373.50 |
Frozen Mouse Embryo | B6;SJL-Tg(Foxl1-cre)1Khk/J Frozen Embryo | $3373.50 |
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