B6.129-Aurka^tm1.1Tvd/J

Stock number: 017729
Product name: AurA^f
Research areas: Apoptosis Research, Research Tools

Description

These floxed mice possess loxP sites flanking exon 2 of the Aurka gene and have applications in studies related to regulation of mitosis and carcinogenesis.

Detailed description

Aurora kinase A is critical in centrosome separation and bipolar spindle formation, and is involved in the regulation of apoptosis. These mice possess loxP sites on either side of exon 2 of the targeted gene. Mice that are homozygous for this allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When these mutant mice are bred to mice that express Cre recombinase, resulting offspring will have exon 2 deleted in the cre-expressing tissue(s).

When bred to a strain with tamoxifen-inducible Cre recombinase expression (see Stock No. 004847 for example), this mutant mouse strain may be useful in studies of mitosis and apoptosis.

Development

A loxP and FRT site flanked targeting vector containing a NEO cassette was utilized in the construction of this mutant. This selection cassette was inserted upstream of exon 2 of the targeted gene, and another loxP site was inserted upstream of exon 3. This construct was electroporated into 129P2/OlaHsd derived E14 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6J blastocysts. Resulting chimeric male animals were backcrossed to wildtype C57BL/6J mice. These AurA^neo mice were then crossed to X-linked CMV-Cre mice, on the mixed C57BL/6J;129S5 genetic background, which express Cre recombinase under the control of the cytomegalovirus promoter knocked into the Hprt locus (Hprt^{tm1(CMV-cre)Brd}), to remove the Neo selection cassette. Mice that retained the loxP site flanked exon 2, AurA^f, were then bred to C57BL/6J mice for 7 generations to remove the Hprt^{tm1(CMV-cre)Brd} allele. Upon arrival at The Jackson Laboratory, the mice were crossed to C57BL/6J (Stock No. 000664) at least once to establish the colony.

Allele

Symbol: Aurka^tm1.1Tvd
Name: targeted mutation 1.1, Terry Van Dyke
MGI accession ID: MGI:3836420
Generation method: Targeted
Attributes: Conditional ready (e.g. floxed); No functional change
Marker symbol: Aurka
Marker name: aurora kinase A
Chromosome: 2
Strain of origin: 129P2/OlaHsd
Molecular note: An frt and loxP flanked neo cassette was inserted into intron 1 and a third loxP site was inserted into intron 2 via homologous recombination. Cre mediated recombination removed the neo cassette leaving exon 2 floxed.