C3HeB/FeJ-Tg(tetO-TAg)1Efr/J

Stock number: 017719
Research areas: Reproductive Biology Research, Research Tools

Description

These tet-Tag transgenic mice express the SV40 large T antigen under the direction of the tetracycline operator. These mice may be useful for studying tumorigenesis and cancer progression.

Detailed description

These transgenic mice express the SV40 large T antigen gene (TAg) under the direction of the tetracycline-responsive element and a cytomegalovirus minimal promoter. When mated to a mutant strain expressing reverse tetracycline-controlled transactivator protein (rtTA) or tetracycline-controlled transactivator protein (tTA), expression of TAg may be regulated with the tetracycline analog doxycycline in the double mutant offspring. Mice that are hemizygous for the transgene are viable, normal in size and do not display any gross physical or behavioral abnormalities. The Donating Investigator has not attempted to make this strain homozygous. When crossed with a Tg(Ins2-rtTA)2Efr strain (e.g. Stock No. 008250), double transgenic mice treated with doxycycline develop hypoglycemia and multiple pancreatic insulinomas.

Allele

Symbol: Tg(tetO-TAg)1Efr
Name: transgene insertion 1, Shimon Efrat
MGI accession ID: MGI:3770670
Generation method: Transgenic
Attributes: Inducible; Inserted expressed sequence
Marker symbol: Tg(tetO-TAg)1Efr
Marker name: transgene insertion 1, Shimon Efrat
Chromosome: UN
Strain of origin: C3HeB/FeJ
Expressed genes: TAg,SV40 large T-antigen,SV40
Molecular note: To generate the transgene, a fragment containing a tandem array of 7 copies of the tetracycline operator sequence and a cytomegalovirus minimal promoter was placed in front of the SV40 large T antigen gene. The promoter does not allow expression of the TAg gene, and transgenic mice do not develop tumors, unless crossed with mice expressing a tetracycline transactivator gene (tTA or rtTA).