These tet-Tag transgenic mice express the SV40 large T antigen under the direction of the tetracycline operator. These mice may be useful for studying tumorigenesis and cancer progression.
Dr. Shimon Efrat, Sackler School of Medicine
Genetic Background | Generation |
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Allele Type |
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Transgenic (Inducible, Inserted expressed sequence) |
These transgenic mice express the SV40 large T antigen gene (TAg) under the direction of the tetracycline-responsive element and a cytomegalovirus minimal promoter. When mated to a mutant strain expressing reverse tetracycline-controlled transactivator protein (rtTA) or tetracycline-controlled transactivator protein (tTA), expression of TAg may be regulated with the tetracycline analog doxycycline in the double mutant offspring. Mice that are hemizygous for the transgene are viable, normal in size and do not display any gross physical or behavioral abnormalities. The Donating Investigator has not attempted to make this strain homozygous.
When crossed with a Tg(Ins2-rtTA)2Efr strain (e.g. Stock No. 008250), double transgenic mice treated with doxycycline develop hypoglycemia and multiple pancreatic insulinomas.
Expressed Gene | TAg, SV40 large T-antigen, SV40 |
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Site of Expression |
Allele Name | transgene insertion 1, Shimon Efrat |
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Allele Type | Transgenic (Inducible, Inserted expressed sequence) |
Allele Synonym(s) | tet-TAg |
Gene Symbol and Name | Tg(tetO-TAg)1Efr, transgene insertion 1, Shimon Efrat |
Gene Synonym(s) | |
Promoter | tetO, tet operator, |
Expressed Gene | TAg, SV40 large T-antigen, SV40 |
Strain of Origin | C3HeB/FeJ |
Chromosome | UN |
Molecular Note | To generate the transgene, a fragment containing a tandem array of 7 copies of the tetracycline operator sequence and a cytomegalovirus minimal promoter was placed in front of the SV40 large T antigen gene. The promoter does not allow expression of the TAg gene, and transgenic mice do not develop tumors, unless crossed with mice expressing a tetracycline transactivator gene (tTA or rtTA). |
Mutations Made By | Dr. Shimon Efrat, Sackler School of Medicine |
When maintaining a live colony, transgene carrier mice may be bred with wildtype (noncarrier) mice from the colony or with C3HeB/FeJ inbred mice (Stock No. 000658). The Donating Investigator has not attempted to make this strain homozygous.
When using the C3HeB/FeJ-Tg(tetO-TAg)1Efr/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #017719 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
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Hemizygous or Non Carrier for Tg(tetO-TAg)1Efr |
Frozen Mouse Embryo | C3HeB/FeJ-Tg(tetO-TAg)1Efr/J | $2595.00 |
Frozen Mouse Embryo | C3HeB/FeJ-Tg(tetO-TAg)1Efr/J | $2595.00 |
Frozen Mouse Embryo | C3HeB/FeJ-Tg(tetO-TAg)1Efr/J | $3373.50 |
Frozen Mouse Embryo | C3HeB/FeJ-Tg(tetO-TAg)1Efr/J | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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