The Kiss1-CreGFP allele expresses a CreGFP fusion protein from the Kiss1 promoter/enhancer elements. These knock-in mice may be useful for visualizing KISS1 secreting neurons and for studying their role in fertility.
Robert A. Steiner, University of Washington, Box 357290
Genetic Background | Generation |
---|---|
|
Allele Type | Gene Symbol | Gene Name |
---|---|---|
Targeted (Recombinase-expressing, Reporter, Null/Knockout) | Kiss1 | KiSS-1 metastasis-suppressor |
The Kiss1-CreGFP allele expresses a CreGFP fusion protein (EGFP and cre fusion protein) from the Kiss1 promoter/enhancer elements. KISS1 (Kisspeptin) mediates the onset of puberty by regulating the feedback effects of gonadotropin-releasing hormone (GnRH) and luteinizing hormone secretion from GnRH neurons. Heterozygous mice are viable and fertile, normal in size and do not display any gross physical or behavioral abnormalities. The DI states that homozygous mice are often infertile. When Kiss1 is induced, EGFP immunofluorescence is observed in the hypothalamic arcuate nucleus (ARC) of both sexes and anteroventral periventricular nucleus (AVPV) in females. When Kiss1-CreGFP mice are bred with mice containing loxP-flanked sequence, cre-mediated recombination will result in deletion of the floxed sequences in the Kiss-expressing neurons of the offspring. These mice may be useful for visualizing KISS1 secreting neurons and for studying their role in fertility.
Update January, 2019:
Kiss1Cre:GFP (v2) (see Stock No. 033169) represents an improved version of this Kiss1tm1.1(cre/EGFP)Stei allele (Stock No. 017701, also called Kiss1Cre:GFP v1), which is prone to ectopic expression when crossed to conditional reporter mice, as well as retains levels of low expression. Unlike the Kiss1tm1.1(cre/EGFP)Stei (v1), homozygotes of the Kiss1Cre:GFP (v2) line are Kiss1 null and infertile. Homozygous Kiss1Cre:GFP (v2) males have diminished testosterone levels compared to heterozygotes.
Barring genetic crosses, the Kiss1Cre:GFP v1 line (Stock No. 017701) remains a useful tool for visualizing Kiss1 neurons for electrophysiological recordings with the GFP-fused cre-reporter transgene. No immunohistochemistry is required to visualize GFP expression in the Kiss1Cre:GFP v1 line line. Also, viral injections into adult v1 mice, as opposed genetic crosses, can be used to gain control of those Kiss1 neurons and thereby avoid developmental ectopic recombination.
A targeting vector was designed to insert a CreGFP fusion protein cassette and frt-flanked neomycin (neo) selection cassette upstream of the 5' translation start of the KiSS-1 metastasis-suppressor (Kiss1) targeted gene. Specifically, this targeting vector contained an enhanced green fluorescent protein (EGFP) and a Cre recombinase. This construct was electroporated into F1 hybrid (129S6/SvEvTac x C57BL/6NCr)-derived G4 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6 blastocysts and chimeric males were bred with C57BL/6 females. These Kiss1-CreGFP mice were bred with FLPe mice (on a C57BL/6 congenic background) to remove the neo selection cassette. Resulting offspring were bred to C57BL/6J mice for at least 3 generations and were maintained on this mixed genetic background. Upon arrival at The Jackson Laboratory, mice were bred with C57BL/6J inbred mice (Stock No. 000664) for at least one generation to establish the colony.
Expressed Gene | GFP, Green Fluorescent Protein, |
---|---|
Expressed Gene | cre, cre recombinase, bacteriophage P1 |
Site of Expression | EGFP immunofluorescence is observed in the hypothalamic arcuate nucleus (ARC) of both sexes and anteroventral periventricular nucleus (AVPV) in females. |
Allele Name | targeted mutation 1.1, Robert A Steiner |
---|---|
Allele Type | Targeted (Recombinase-expressing, Reporter, Null/Knockout) |
Allele Synonym(s) | Kiss1-CreGFP |
Gene Symbol and Name | Kiss1, KiSS-1 metastasis-suppressor |
Gene Synonym(s) | |
Expressed Gene | GFP, Green Fluorescent Protein, |
Expressed Gene | cre, cre recombinase, bacteriophage P1 |
Site of Expression | EGFP immunofluorescence is observed in the hypothalamic arcuate nucleus (ARC) of both sexes and anteroventral periventricular nucleus (AVPV) in females. |
Strain of Origin | (129S6/SvEvTac x C57BL/6NCrl)F1 |
Chromosome | 1 |
Molecular Note | A targeting vector was designed to insert a CreGFP fusion protein cassette and frt-flanked neomycin (neo) selection cassette upstream of the 5' translation start of the KiSS-1 metastasis-suppressor (Kiss1) targeted gene. Specifically, this targeting vector contained an enhanced green fluorescent protein (EGFP) and a Cre recombinase. This construct was electroporated into F1 hybrid (129S6/SvEvTac x C57BL/6NCr)-derived G4 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6 blastocysts and chimeric males were bred with C57BL/6 females. These Kiss1-CreGFP mice were bred with FLPe mice (on a C57BL/6 congenic background) to remove the neo selection cassette. When Kiss1 is induced, EGFP immunofluorescence is observed in the hypothalamic arcuate nucleus (ARC) of both sexes and anteroventral periventricular nucleus (AVPV) in females. Homozygotes are often infertile, but heterozygotes display normal fertility. |
When maintaining a live colony heterozygous mice may be bred together. The DI states that homozygous are often infertile.
When using the Kiss1-CreGFP, Kiss1Cre:GFP v1
mouse strain in a publication, please cite the originating article(s) and include JAX stock #017701 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous or wildtype for Kiss1<tm1.1(cre/EGFP)Stei> |
Frozen Mouse Embryo | STOCK Kiss1<tm1.1(cre/EGFP)Stei>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | STOCK Kiss1<tm1.1(cre/EGFP)Stei>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | STOCK Kiss1<tm1.1(cre/EGFP)Stei>/J Frozen Embryo | $3373.50 |
Frozen Mouse Embryo | STOCK Kiss1<tm1.1(cre/EGFP)Stei>/J Frozen Embryo | $3373.50 |
Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
What information were you hoping to find through your search?
How easy was it to find what you were looking for?
We may wish to follow up with you. Enter your email if you are happy for us to connect and reachout to you with more questions.
Please Enter a Valid Email Address
Thank you for sharing your feedback! We are working on improving the JAX Mice search. Come back soon for exciting changes.