These floxed mutant mice possess loxP sites flanking exons 11 and 12 of the Foxp1 gene. This strain may be useful for generating conditional mutations in applications related to T cell development and neuronal development.
Philip W Tucker, The University of Texas at Austin - ICMB
The targeted Foxp1 gene encodes a forkhead box transcription factor that acts as a transcriptional repressor and is important in neuronal development. Mutations in this gene have been associated with mental retardation with language impairment and autistic features. These mice possess loxP sites on either side of exons 11 and 12 of the targeted Foxp1 gene. Mice that are homozygous for this allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When these mutant mice are bred to mice that express Cre recombinase, resulting offspring will have exons 11 and 12 deleted in the cre-expressing tissue(s).
When bred to a strain with Cre recombinase expression in CD4+ T cell development, this mutant mouse strain may be useful in studies of abnormal T cell development and activation.
A targeting vector containing a FRT site flanked PGK-Neo selection cassette and a loxP site was utilized in the construction of this mutant. This selection cassette was inserted upstream of exon 11 of the targeted gene, and another loxP site was inserted downstream of exon 12. This construct was electroporated into unspecified embryonic stem (ES) cells. Correctly targeted ES cells were injected into recipient blastocysts. The resulting chimeric animals were crossed to unspecified mice expressing FLP recombinase. Mice that retained the loxP site flanked exons 11 and 12 were then bred to C57BL/6 mice to remove the FLP recombinase allele/transgene. The mice were backcrossed to C57BL/6 for 5 generations, and then crossed to mice on the B6.129X1 background by the donating lab (see SNP notes below). Upon arrival, sperm was cryopreserved. To establish our live colony, an aliquot of frozen sperm was used to fertilize C57BL/6J oocytes (Stock No. 000664).
A 48 SNP (single nucleotide polymorphism) panel analysis, with 43 markers covering all 19 chromosomes and the X chromosome, as well as 5 markers that distinguish between the C57BL/6J and C57BL/6N substrains, was performed on the rederived living colony at The Jackson Laboratory Repository. Three of the 43 markers throughout the genome were segregating with 129, suggesting an incomplete backcross. Also, 2 of 5 markers that determine C57BL/6J from C57BL/6N were found to be segregating. These data suggest the mice sent to The Jackson Laboratory Repository were on a mixed C57BL/6J ; C57BL/6N genetic background.
|Allele Name||targeted mutation 1.1, Philip W Tucker|
|Allele Type||Targeted (Conditional ready (e.g. floxed), No functional change)|
|Gene Symbol and Name||Foxp1, forkhead box P1|
|Strain of Origin||Not Specified|
|Molecular Note||An frt flanked neo cassette with a 3' loxP site was inserted upstream of exon 11 and an additional loxP site was inserted downstream of exon 12. Flp mediated recombination removed the neo cassette leaving exons 11 and 12 floxed.|
When maintaining a live colony, these mice can be bred as homozygotes.
When using the Foxp1 flox mouse strain in a publication, please cite the originating article(s) and include JAX stock #017699 in your Materials and Methods section.