The Shank3B- knockout allele has a neo cassette replacing the PDZ domain (exons 13-16) of the Shank3 gene, resulting altered expression of the Shank3b isoform. These mice may be useful for studying behavioral abnormalities associated with autism spectrum disorder.
Guoping Feng, Massachusetts Institute of Technology
Genetic Background | Generation |
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F5+N6F1
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Allele Type | Gene Symbol | Gene Name |
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Targeted | Shank3 | SH3 and multiple ankyrin repeat domains 3 |
Starting at:
$255.00 Domestic price for female 4-week |
333.51 Domestic price for breeder pair |
SHANK3 is a synaptic scaffolding protein, expressed in the postsynaptic density (PSD) of excitatory synapses. Five intragenic promoters exist, allowing for extensive alternative splicing. SHANK3 mutations have been identified in cases of intellectual disability such as autism spectrum disorder (ASD), Phelan-McDermid syndrome, and Schizophrenia. In this strain a neo cassette replaces exons 13-16 of the Shank3 gene. Specifically, the PDZ domain is disrupted resulting in a deficiency of Shank3α and Shank3β, and a reduction in expression of the Shank3γ isoform. Mice display abnormal social behaviors, excessive grooming, and self-injurious repetitive behaviors. Shank3b-/- mice also exhibit defects at striatal synapses and cortical-striatal circuits, as well as changes in medium spiny neuron morphology and density. Homozygotes are viable and fertile. These mice may be useful for studying behavioral abnormalities associated with ASD.
A targeting vector was designed to replace exons 13-16 of SH3/ankyrin domain 3 (Shank3) gene with a neomycin resistance (neo) cassette. The construct was electroporated into (129X1/SvJ x 129S1/Sv)F1-Kitl+-derived R1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6 blastocysts and the resulting chimeric males were bred to C57BL/6J females. These Shank3b mice were then intercrossed for five generations and maintained on a mixed C57BL/6J x 129 background prior to sending to The Jackson Laboratory Repository. Upon arrival, mice were additionally backcrossed to C57BL/6J inbred mice (Stock No. 000664) using a marker-assisted, speed congenic approach to establish this congenic line.
Allele Name | targeted mutation 2, Guoping Feng |
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Allele Type | Targeted |
Allele Synonym(s) | Shank3/F; Shank3B- |
Gene Symbol and Name | Shank3, SH3 and multiple ankyrin repeat domains 3 |
Gene Synonym(s) | |
Strain of Origin | (129X1/SvJ x 129S1/Sv)F1-Kitl+ |
Chromosome | 15 |
Molecular Note | The sequence from exons 13 through 16, which encode the PDZ domain, was replaced with a neo cassette. The absence of alpha and beta isoform expression and reduced gamma isoform expression were confirmed by western blot analysis on brain extracts. |
Mutant mice were bred to C57BL/6J inbred mice (Stock No. 000664) using a marker-assisted, speed congenic approach to establish this congenic line. When maintaining the live congenic colony, homozygous mice may be bred together. If breeding homozygous mice, note that homozygous mice exhibit excessive grooming that result in skin lesions.
When using the Shank3B- mouse strain in a publication, please cite the originating article(s) and include JAX stock #017688 in your Materials and Methods section.
Service/Product | Description | Price |
---|---|---|
Heterozygous or wildtype for Shank3<tm2Gfng> |
Frozen Mouse Embryo | B6.129-Shank3<tm2Gfng>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6.129-Shank3<tm2Gfng>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6.129-Shank3<tm2Gfng>/J Frozen Embryo | $3373.50 |
Frozen Mouse Embryo | B6.129-Shank3<tm2Gfng>/J Frozen Embryo | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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