C57BL/6N-Tg(Thy1-SNCA)15Mjff/J

Stock number: 017682
Product name: mThy1-hSNCA line 15 , hα-Syn-Tg
Research areas: Neurobiology Research

Description

This transgenic line expresses human SNCA under the direction of a mouse Thy-1 promoter. Applications exist in studies related to Parkinson's disease and neurodegeneration elicited by the aggregation of SNCA.

Detailed description

mThy1-hSNCA transgenic mice express the human α-synuclein gene under the direction of the mouse thymus cell antigen 1 promoter. Hemizygotes are viable, fertile, and normal in size. This line carries 1-2 copies of the transgene which is integrated on mouse Chromosome 11 (40456787-40495044) and results in a 38.4 kb deletion (PMID 32170061). These mice may be useful for studying Parkinson's disease pathogenesis and neurodegeneration elicited by the toxic effects of aggregation and somatic accumulation of SNCA.

Development

The mThy1-hSNCA transgene consists of the human α-synuclein (SNCA) gene driven by a mouse thymus cell antigen 1 (Thy-1) promoter. The construct was microinjected into fertilized C57BL/6NTac oocytes and males from founder line 15 were bred to C57BL/6NTac females to establish the colony. Upon arrival at The Jackson Laboratory, mice were bred to C57BL/6NJ inbred mice (Stock No. 005304) for at least one generation.

Analysis of the integration site indicates insertion occurred at Chr11:40456787-40495044 and resulted in a 38.4 Kb deletion (PMID 32170061).

Allele

Symbol: Tg(Thy1-SNCA)15Mjff
Name: transgene insertion 15, The Michael J Fox Foundation
MGI accession ID: MGI:5308782
Generation method: Transgenic
Attributes: Inserted expressed sequence; Humanized sequence
Marker symbol: Tg(Thy1-SNCA)15Mjff
Marker name: transgene insertion 15, The Michael J Fox Foundation
Chromosome: 11
Strain of origin: C57BL/6NTac
Expressed genes: SNCA,synuclein alpha,human
Molecular note: The transgene consists of the human alpha-synuclein (SNCA) gene driven by a mouse thymus cell antigen 1 (Thy-1) promoter. Line 15 was generated. Transgene insertion occurred on Chr 11, causing a 38 Kb deletion.