These compound mutant mice carry both the Prkdcscid (scid) mutation and a transgene harboring an enhanced green fluorescent protein (EGFP) cDNA under the control of a chicken beta-actin promoter and cytomegalovirus enhancer (CAG).

Mice hemizygous for the EGFP transgene exhibit a robust and widespread expression of green fluorescent. Animals homozygous for scid and hemizygous for GFP are viable and fertile and exhibit the same immunodeficient characteristics of the parental NOD Prkdcscid strain (e.g. T, B, and NK cell deficiencies - see Stock No. <a href="https://www.jax.org/strain/001303">001303</a>). The Donating Investigator reports that mice homozygous for the Tg(CAG-EGFP)1Osb transgene are not viable.

These mice can act as recipients of allogeneic and xenogeneic grafts and perhaps more importantly, donors of fluorescent-labeled cells, making them an ideal model for cell transfer experiments. 

In an attempt to offer alleles on well-characterized or multiple genetic backgrounds, alleles are frequently moved to a genetic background different from that on which an allele was first characterized. This is the case for the strain above. It should be noted that the phenotype could vary from that originally described. We will modify the strain description if necessary as published results become available.

This compound mutant strain combines the immunodeficiency features of the Prkdcscid mutation and widespread fluorescent cell labeling capabilities of CAG-EGFP on the NOD genetic background. These mice are effective recipients of allogeneic and xenogenic grafts, and are an excellent source of fluorescent-labeled cells for transfer experiments.

Typically mice are recovered in 12-16 weeks. Contact Customer Service to place an order or for more information.

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NOD.Cg-Prkdc<scid> Tg(CAG-EGFP)1Osb/KupwJ