NOD.Cg-Prkdc^scid Tg(CAG-EGFP)1Osb/KupwJ

Stock number: 017619
Product name: NOD/SCID-GFP
Research areas: Diabetes and Obesity Research, Immunology, Inflammation and Autoimmunity Research, Internal/Organ Research, Research Tools, Virology Research

Description

This compound mutant strain combines the immunodeficiency features of the Prkdc^scid mutation and widespread fluorescent cell labeling capabilities of CAG-EGFP on the NOD genetic background. These mice are effective recipients of allogeneic and xenogenic grafts, and are an excellent source of fluorescent-labeled cells for transfer experiments.

Detailed description

These compound mutant mice carry both the Prkdc^scid (scid) mutation and a transgene harboring an enhanced green fluorescent protein (EGFP) cDNA under the control of a chicken beta-actin promoter and cytomegalovirus enhancer (CAG).

Mice hemizygous for the EGFP transgene exhibit a robust and widespread expression of green fluorescent. Animals homozygous for scid and hemizygous for GFP are viable and fertile and exhibit the same immunodeficient characteristics of the parental NOD Prkdc^scid strain (e.g. T, B, and NK cell deficiencies - see Stock No. 001303). The Donating Investigator reports that mice homozygous for the Tg(CAG-EGFP)1Osb transgene are not viable.

These mice can act as recipients of allogeneic and xenogeneic grafts and perhaps more importantly, donors of fluorescent-labeled cells, making them an ideal model for cell transfer experiments.

In an attempt to offer alleles on well-characterized or multiple genetic backgrounds, alleles are frequently moved to a genetic background different from that on which an allele was first characterized. This is the case for the strain above. It should be noted that the phenotype could vary from that originally described. We will modify the strain description if necessary as published results become available.

Development

A CAG-EGFP transgenic mouse (Stock No. 003291) was intercrossed with NOD background Prkdc^scid (Stock No. 001303) animals for more than 10 generations by the donating laboratory.

Allele

Symbol: Tg(CAG-EGFP)1Osb
Name: transgene insertion 1, Research Institute for Microbial Diseases, Osaka University
MGI accession ID: MGI:2686773
Generation method: Transgenic
Attributes: Reporter
Marker symbol: Tg(CAG-EGFP)1Osb
Marker name: transgene insertion 1, Research Institute for Microbial Diseases, Osaka University
Chromosome: 15
Strain of origin: C57BL/6
Expressed genes: GFP,Green Fluorescent Protein,
Site of expression: All tissues, with the exception of erythrocytes and hair, appear green under excitation light.
Molecular note: cDNA encoding enhanced green fluorescent protein (EGFP) was adjoined to the chicken beta actin promoter and cytomegalovirus enhancer. A bovine globin polyadenylation signal was also included in the construct.

Allele

Symbol: Prkdc^scid
Name: severe combined immunodeficiency
MGI accession ID: MGI:1857113
Generation method: Spontaneous
Marker symbol: Prkdc
Marker name: protein kinase, DNA activated, catalytic polypeptide
Chromosome: 16
Strain of origin: C.BKa-Igh^b/Icr
Site of expression: T and B lymphocytes.
Molecular note: A T-to-A transversion point mutation at a position corresponding to codon 4046 (codon 4095 in transcript ENSMUST00000023352.8) created a premature stop codon (p.Y4046*).