Goldenticket (Tmem173gt) is an I199N missense mutant allele of the Sting gene (formerly, Tmem173). Homozygotes do not produce IFN-β in response to cyclic dinucleotides or Listeria monocytogenes infection. This strain may be useful in studies related to the detection of cytosolic pathogen DNA, innate immune responses and the exploration of the tumor microenvironment.
Russell Vance, University of California, Berkeley
Genetic Background | Generation |
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000664 C57BL/6J |
?+N2F5
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Allele Type | Gene Symbol | Gene Name |
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Chemically induced (ENU) | Sting1 | stimulator of interferon response cGAMP interactor 1 |
Goldenticket (Tmem173gt) is a chemically-induced (ENU) mutant allele of the transmembrane protein 173 locus (Tmem173 or Sting) harboring a missense mutation in exon 6 - which results in an isoleucine-to-asparagine change in amino acid 199 in the C-terminal of the protein. No gene product (protein) is detected by Western blot analysis of bone marrow-derived macrophages from homozygotes. Mice homozygous for the mutation are viable and fertile. Thioglycolate-elicited peritoneal macrophages isolated from homozygotes do not produce type I interferons (IFN-β) in response to cyclic dinucleotides (c-di-GMP) or Listeria monocytogenes infection. In contrast, MPYS-/- mice (Stock No. 025805) exhibit decreased production of IFNβ after Listeria monocytogenes infection compared to controls, and regain normal production 24 hours later.
Following multidose N-ethyl-N-nitrosourea (ENU) treatments to induce mutations in founder C57BL/6J mice, a forward genetic screen was utilized to identify mice exhibiting a recessive mutation causing changes in production of type I interferons in response to Listeria monocytogenes infection. Mouse 1009 was identified in the screening, and called Gt or goldenticket. Sequencing of multiple loci revealed a single base pair change, T596A, in exon 6 of Tmem173. This missense mutation results in isoleucine-to-asparagine change in amino acid 199, in the C-terminal of the protein.
Upon arrival at The Jackson Laboratory, the mice were crossed to C57BL/6J (Stock No. 000664) at least once to establish the colony.
Allele Name | goldenticket |
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Allele Type | Chemically induced (ENU) |
Allele Synonym(s) | Goldenticket (Gt); StingGt; Tmem173I199N |
Gene Symbol and Name | Sting1, stimulator of interferon response cGAMP interactor 1 |
Gene Synonym(s) | |
Strain of Origin | C57BL/6 |
Chromosome | 18 |
Molecular Note | ENU mutagenesis induced a T to A transversion that results in the amino acid substitution of asparagine for isoleucine at position 199 (I199N). The absence of protein expression was confirmed by western blot analysis on macrophage extracts. |
Mutations Made By | Russell Vance, University of California, Berkeley |
When maintaining a live colony, these mice can be bred as homozygotes.
When using the Goldenticket (Gt) , StingGt mouse strain in a publication, please cite the originating article(s) and include JAX stock #017537 in your Materials and Methods section.
Service/Product | Description | Price |
---|---|---|
Heterozygous for Tmem173<gt> |
Frozen Mouse Embryo | C57BL/6J-Sting1<gt>/J | $2595.00 |
Frozen Mouse Embryo | C57BL/6J-Sting1<gt>/J | $2595.00 |
Frozen Mouse Embryo | C57BL/6J-Sting1<gt>/J | $3373.50 |
Frozen Mouse Embryo | C57BL/6J-Sting1<gt>/J | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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