These mice harbor a point mutation in the endogenous Lepr gene resulting in an inability of the targeted gene to activate STAT5. Reported phenotypic traits include increased weight gain and impaired estrous cycling.
Martin G Myers, University of Michigan Medical School
Genetic Background | Generation |
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|
Allele Type | Gene Symbol | Gene Name |
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Targeted | Lepr | leptin receptor |
This strain expresses a Tyr1077 to Phe1077 substitution which abolishes STAT5 activation by the mutant protein.
Homozygous females exhibit increased body weight on chow diet and impaired estrous cycling, but are fertile. Homozygotes on a normal chow diet display slightly increased adipose mass and serum leptin levels. When fed a high fat diet, homozygotes are slightly heavier and have an increased food intake (approximately 5-10%) when compared to controls.
Mice that are heterozygous for the targeted mutation are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities.
A targeting vector containing a Tyr1077 to Phe1077 substitution and a floxed NEO cassette was electroporated into 129 derived R1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into recipient blastocysts. The resulting chimeric animals were crossed to C57BL/6J mice. The mice may have been bred to FVB, and were then backcrossed to C57BL/6J for 9 generations. Upon arrival at The Jackson Laboratory, the mice were crossed to C57BL/6J (Stock No. 000664) at least once to establish the colony.
Allele Name | targeted mutation 5, Martin G Myers, Jr |
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Allele Type | Targeted |
Allele Synonym(s) | leprF1077 |
Gene Symbol and Name | Lepr, leptin receptor |
Gene Synonym(s) | |
Promoter | Lepr, leptin receptor, mouse, laboratory |
Strain of Origin | (129X1/SvJ x 129S1/Sv)F1-Kitl+ |
Chromosome | 4 |
Molecular Note | The targeting vector contained a nucleotide substitution that results in the amino acid substitution of phenyalanine for tyrosine at position 1077 (Y1077F) and a floxed NEO cassette. This mutation is predicted to abolish STAT5 activation by the mutated protein. |
When maintaining a live colony, these mice can be bred as heterozgyotes or homozygotes. Female homozygotes exhibit increased body weight on chow diet and impaired estrous cycling.
When using the B6.129-Leprtm5Mgmj/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #017527 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous for Lepr<tm5Mgmj> |
Frozen Mouse Embryo | B6.129-Lepr<tm5Mgmj>/J | $2595.00 |
Frozen Mouse Embryo | B6.129-Lepr<tm5Mgmj>/J | $2595.00 |
Frozen Mouse Embryo | B6.129-Lepr<tm5Mgmj>/J | $3373.50 |
Frozen Mouse Embryo | B6.129-Lepr<tm5Mgmj>/J | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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