These mice harbor a point mutation in the endogenous Lepr gene resulting in an inability of the targeted gene to activate STAT5. Reported phenotypic traits include increased weight gain and impaired estrous cycling.
Martin G Myers, University of Michigan Medical School
This strain expresses a Tyr1077 to Phe1077 substitution which abolishes STAT5 activation by the mutant protein.
Homozygous females exhibit increased body weight on chow diet and impaired estrous cycling, but are fertile. Homozygotes on a normal chow diet display slightly increased adipose mass and serum leptin levels. When fed a high fat diet, homozygotes are slightly heavier and have an increased food intake (approximately 5-10%) when compared to controls.
Mice that are heterozygous for the targeted mutation are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities.
A targeting vector containing a Tyr1077 to Phe1077 substitution and a floxed NEO cassette was electroporated into 129 derived R1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into recipient blastocysts. The resulting chimeric animals were crossed to C57BL/6J mice. The mice may have been bred to FVB, and were then backcrossed to C57BL/6J for 9 generations. Upon arrival at The Jackson Laboratory, the mice were crossed to C57BL/6J (Stock No. 000664) at least once to establish the colony.
|Allele Name||targeted mutation 5, Martin G Myers, Jr|
|Gene Symbol and Name||Lepr, leptin receptor|
|Promoter||Lepr, leptin receptor, mouse, laboratory|
|Strain of Origin||(129X1/SvJ x 129S1/Sv)F1-Kitl+|
|Molecular Note||The targeting vector contained a nucleotide substitution that results in the amino acid substitution of phenyalanine for tyrosine at position 1077 (Y1077F) and a floxed NEO cassette. This mutation is predicted to abolish STAT5 activation by the mutated protein.|
When maintaining a live colony, these mice can be bred as heterozgyotes or homozygotes. Female homozygotes exhibit increased body weight on chow diet and impaired estrous cycling.
When using the B6.129-Leprtm5Mgmj/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #017527 in your Materials and Methods section.