These Nos1cre knock-in mice may be useful for generating conditional mutations for studying metabolism and energy homeostasis.
Martin G Myers, University of Michigan Medical School
Genetic Background | Generation |
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N10+pN2F3
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Allele Type | Gene Symbol | Gene Name |
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Targeted (Recombinase-expressing) | Nos1 | nitric oxide synthase 1, neuronal |
This Nos1cre strain expresses Cre recombinase from the endogenous Nos1, neuronal nitric oxide synthase 1, locus. Mice that are heterozygous or homozygous for the targeted mutation are viable and fertile. NOS1-expressing tissues typically include discrete populations of neurons in the cerebellum, olfactory, bulb, hippocampus, cortex, striatum, basal forebrain and brain stem. Users should be cautioned that periodic expression during gametogenesis is also observed (see below). When crossed with a strain containing a loxP site-flanked sequence, Cre-mediated recombination results in tissue-specific deletion of the sequence in the offspring.
Luo et al. 2020 Neuron 106:37 Table 1 shows germline recombination in offspring (F2) of Cre;floxed double mutant (F1) mice bred to floxed and/or wildtype mice. The authors also note that in general, the frequency of recombination in Cre;floxed double mutant germline cells appears to be considerably higher than in zygotes produced by breeding Cre mice to floxed mice.
This reports that Nos1cre;floxed double mutant females bred to floxed males produced some offspring with germline deletion of the floxed allele. Cre expression in the male germline was not determined. As such, for Cre-lox experiments and to avoid/minimize germline deletion of the floxed allele, researchers may consider breeding Nos1cre males to floxed females.
If the recombinase activity pattern of this allele is further characterized by the Genetic Resource Science group at The Jackson Laboratory, such findings will be reported on the Mouse Genome Informatics (MGI) Allele Detail entry. This same information may also be found searching the MGI Recombinase Activity and MGI Gene Expression + Recombinase Activity Comparison Matrix.
IRES and cre recombinase sequence was inserted in the 3' UTR of the Nos1 gene. The construct was electroporated into 129 derived R1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6 blastocysts. The resulting chimeric animals were crossed to C57BL/6J mice, and then backcrossed to C57BL/6J for 9 generations. These mice contain a FRT-flanked NEO downstream of the Nos1 gene. Upon arrival at The Jackson Laboratory, the mice were crossed to C57BL/6J (Stock No. 000664) at least once to establish the colony.
Expressed Gene | cre, cre recombinase, bacteriophage P1 |
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Site of Expression | Includes discrete populations of neurons in the cerebellum, olfactory, bulb, hippocampus, cortex, striatum, basal fore-brain and brain stem. Periodic expression during gametogenesis is also observed in gametes. |
Allele Name | targeted mutation 1, Martin G Myers, Jr |
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Allele Type | Targeted (Recombinase-expressing) |
Allele Synonym(s) | Nos1cre; Nos1-ires-cre |
Gene Symbol and Name | Nos1, nitric oxide synthase 1, neuronal |
Gene Synonym(s) | |
Expressed Gene | cre, cre recombinase, bacteriophage P1 |
Site of Expression | Includes discrete populations of neurons in the cerebellum, olfactory, bulb, hippocampus, cortex, striatum, basal fore-brain and brain stem. Periodic expression during gametogenesis is also observed in gametes. |
Strain of Origin | (129X1/SvJ x 129S1/Sv)F1-Kitl+ |
Chromosome | 5 |
Molecular Note | An IRES-cre-Frt-neo-Frt cassette was inserted after the stop codon of the final 3' exon of the Nos1 gene by homologous recombination in R1 ES cells. Correctly targeted ES cells were injected into C57BL/6 blastocysts. The resulting chimeric animals were crossed to C57BL/6J mice, and then backcrossed to C57BL/6J for 9 generations. |
Mutations Made By | Martin Myers, University of Michigan Medical School |
When maintaining a live colony, these mice can be bred as heterozygotes or homozygotes.
Users should be cautioned that periodic Cre recombinase expression during gametogenesis is observed.
For Cre-lox experiments and to avoid/minimize germline deletion of the floxed allele, researchers may consider breeding Nos1cre males to floxed females. See Detailed Description for more details.
When using the Nos1cre mouse strain in a publication, please cite the originating article(s) and include JAX stock #017526 in your Materials and Methods section.
Service/Product | Description | Price |
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Heterozygous or wildtype for Nos1<tm1(cre)Mgmj> |
Frozen Mouse Embryo | B6.129-Nos1<tm1(cre)Mgmj>/J | $2595.00 |
Frozen Mouse Embryo | B6.129-Nos1<tm1(cre)Mgmj>/J | $2595.00 |
Frozen Mouse Embryo | B6.129-Nos1<tm1(cre)Mgmj>/J | $3373.50 |
Frozen Mouse Embryo | B6.129-Nos1<tm1(cre)Mgmj>/J | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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