Overview

Also Known As:Nos1^cre

These Nos1^cre knock-in mice may be useful for generating conditional mutations for studying metabolism and energy homeostasis.

Donating Investigator

Martin G Myers, University of Michigan Medical School

Genetic overview

Genetic Background	Generation
	N10+pN2F3 (2019-03-01 00:00:00)

Nos1^tm1(cre)Mgmj

Allele Type	Gene Symbol	Gene Name
Targeted (Recombinase-expressing)	Nos1	nitric oxide synthase 1, neuronal

View Genetics

Research Applications

Neurobiology Research

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Base Price

Starting at:

$305.00 Domestic price for female 4-week

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Details

Detailed Description

This Nos1^cre strain expresses Cre recombinase from the endogenous Nos1, neuronal nitric oxide synthase 1, locus. Mice that are heterozygous or homozygous for the targeted mutation are viable and fertile. NOS1-expressing tissues typically include discrete populations of neurons in the cerebellum, olfactory, bulb, hippocampus, cortex, striatum, basal forebrain and brain stem. Users should be cautioned that periodic expression during gametogenesis is also observed (see below). When crossed with a strain containing a loxP site-flanked sequence, Cre-mediated recombination results in tissue-specific deletion of the sequence in the offspring.

Luo et al. 2020 Neuron 106:37 Table 1 shows germline recombination in offspring (F2) of Cre;floxed double mutant (F1) mice bred to floxed and/or wildtype mice. The authors also note that in general, the frequency of recombination in Cre;floxed double mutant germline cells appears to be considerably higher than in zygotes produced by breeding Cre mice to floxed mice.
This reports that Nos1^cre;floxed double mutant females bred to floxed males produced some offspring with germline deletion of the floxed allele. Cre expression in the male germline was not determined. As such, for Cre-lox experiments and to avoid/minimize germline deletion of the floxed allele, researchers may consider breeding Nos1^cre males to floxed females.
If the recombinase activity pattern of this allele is further characterized by the Genetic Resource Science group at The Jackson Laboratory, such findings will be reported on the Mouse Genome Informatics (MGI) Allele Detail entry. This same information may also be found searching the MGI Recombinase Activity and MGI Gene Expression + Recombinase Activity Comparison Matrix.

Development

IRES and cre recombinase sequence was inserted in the 3' UTR of the Nos1 gene. The construct was electroporated into 129 derived R1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6 blastocysts. The resulting chimeric animals were crossed to C57BL/6J mice, and then backcrossed to C57BL/6J for 9 generations. These mice contain a FRT-flanked NEO downstream of the Nos1 gene. Upon arrival at The Jackson Laboratory, the mice were crossed to C57BL/6J (Stock No. 000664) at least once to establish the colony.

Expression Data

Expressed Gene	cre, cre recombinase, bacteriophage P1
Site of Expression	Includes discrete populations of neurons in the cerebellum, olfactory, bulb, hippocampus, cortex, striatum, basal fore-brain and brain stem. Periodic expression during gametogenesis is also observed in gametes.

Control Suggestions

000664 C57BL/6J

Additional Information

Considerations for Choosing Controls

Genetics

Nos1^tm1(cre)Mgmj

Allele Symbol: Nos1^tm1(cre)Mgmj

Allele Name	targeted mutation 1, Martin G Myers, Jr
Allele Type	Targeted (Recombinase-expressing)
Allele Synonym(s)	Nos1^cre; Nos1-ires-cre
Gene Symbol and Name	Nos1, nitric oxide synthase 1, neuronal
Gene Synonym(s)
Expressed Gene	cre, cre recombinase, bacteriophage P1
Site of Expression	Includes discrete populations of neurons in the cerebellum, olfactory, bulb, hippocampus, cortex, striatum, basal fore-brain and brain stem. Periodic expression during gametogenesis is also observed in gametes.
Strain of Origin	(129X1/SvJ x 129S1/Sv)F1-Kitl⁺
Chromosome	5
Molecular Note	An IRES-cre-Frt-neo-Frt cassette was inserted after the stop codon of the final 3' exon of the Nos1 gene by homologous recombination in R1 ES cells. Correctly targeted ES cells were injected into C57BL/6 blastocysts. The resulting chimeric animals were crossed to C57BL/6J mice, and then backcrossed to C57BL/6J for 9 generations.
Mutations Made By	Martin Myers, University of Michigan Medical School

Disease/Phenotype

Disease Terms

Research Areas By Phenotype

This mouse can be used to support research in many areas including:

Neurobiology Research
- Cre-lox System
  - Cre Recombinase expression in neural tissue

Mammalian Phenotype Terms by Genotype

The following phenotype relates to a compound genotype created using this strain

Genotype: Nos1^tm1(cre)Mgmj/Nos1⁺
B6.129-Nos1

normal phenotype

no abnormal phenotype detected
- Normal - mice are viable and fertile; no behavioral or gross physical abnormalities have been observed

References

Additional - Nos1^tm1(cre)Mgmj related

Technical Support

CONTACT TECHNICAL SUPPORT

Genotyping Protocols
- Separated PCR:Nos1
- Genotyping resources and troubleshooting
Breeding Considerations

When maintaining a live colony, these mice can be bred as heterozygotes or homozygotes.

Users should be cautioned that periodic Cre recombinase expression during gametogenesis is observed.

For Cre-lox experiments and to avoid/minimize germline deletion of the floxed allele, researchers may consider breeding Nos1^cre males to floxed females. See Detailed Description for more details.
- Additional Breeding and Husbandry Support
Mating System
- Homozygote x Homozygote
Citation
When using the Nos1^cre mouse strain in a publication, please cite the originating article(s) and include JAX stock #017526 in your Materials and Methods section.

Animal Health Reports

Facility Barrier Level Descriptions

AX12 (Maximum)

Pricing & Availability

Available

Domestic
International

Domestic Pricing for

Commercial & For-Profit

Not-For-Profit & Academic

International Pricing for

Commercial & For-Profit

Not-For-Profit & Academic

Live Mouse

Age

Genotype

Price

weeks

Cryorecovery - Pricing

Service/Product	Description	Price
	Heterozygous or wildtype for Nos1<tm1(cre)Mgmj>

Related Products and Services

Frozen Mouse Embryo	B6.129-Nos1<tm1(cre)Mgmj>/J	$2595.00
Frozen Mouse Embryo	B6.129-Nos1<tm1(cre)Mgmj>/J	$2595.00
Frozen Mouse Embryo	B6.129-Nos1<tm1(cre)Mgmj>/J	$3373.50
Frozen Mouse Embryo	B6.129-Nos1<tm1(cre)Mgmj>/J	$3373.50

Payment Terms and Conditions

Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.

The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.

Terms Of Use

General Terms and Conditions

Questions about Terms of Use

Additional Use Restrictions Apply

Use of MICE by companies or for-profit entities requires a no-fee JAX Leap License prior to shipping.

Licensing Information

Phone: 207-288-6470

Email: TechTran@jax.org

Also Known As:Nos1cre

Donating Investigator

Genetic overview

Research Applications

Base Price

Detailed Description

Development

Expression Data

Control Suggestions

Additional Information

Nos1tm1(cre)Mgmj

Allele Symbol: Nos1tm1(cre)Mgmj

Disease Terms

Research Areas By Phenotype

This mouse can be used to support research in many areas including:

Mammalian Phenotype Terms by Genotype

Genotype: Nos1tm1(cre)Mgmj/Nos1+B6.129-Nos1

normal phenotype

References

Additional - Nos1tm1(cre)Mgmj related

Genotyping Protocols

Breeding Considerations

Mating System

Citation

Animal Health Reports

Live Mouse

Cryorecovery - Pricing

Related Products and Services

Payment Terms and Conditions

The Jackson Laboratory's Genotype Promise

Terms Of Use

Additional Use Restrictions Apply

Licensing Information

Also Known As:Nos1^cre

Nos1^tm1(cre)Mgmj

Allele Symbol: Nos1^tm1(cre)Mgmj

Genotype: Nos1^tm1(cre)Mgmj/Nos1⁺
B6.129-Nos1

Additional - Nos1^tm1(cre)Mgmj related