These microRNA cluster 24(miR322) conditional mutant mice are designed to generate a null allele or a lacZ tagged null allele when combined with Flp or Cre recombinase expressing strains. This mutant mouse strain may be useful in studies of microRNA biology.
Michael T McManus, University of California, San Francisco
Genetic Background | Generation |
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Allele Type | Gene Symbol | Gene Name |
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Targeted (Conditional ready (e.g. floxed), Reporter, Null/Knockout) | Mirc24 | microRNA cluster 24, including Mir351 through Mir322 |
Mice that are homozygous for the targeted mutation are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When combined with a Flp recombinase-expressing strain,
the lacZ and neomycin genes are removed leaving an FRT site and the loxP-flanked miR322 cluster (Mirc24) stem loop. A further cross to a Cre-recombinase-expressing strain generates the null allele. When combined with a Cre-recombinase-expressing strain, the neomycin cassette and miR322 cluster stem loop are removed leaving a lacZ tagged null allele (FRT-lacZ-loxP). This mutant mouse strain may be useful in studies of microRNA biology.
A targeting vector was designed to insert an FRT site followed by a lacZ gene, a loxP site, a neomycin cassette, an FRT site and a loxP site (FRT-lacZ-loxP-Neo-FRT-loxP) upstream of the miR322cluster (Mirc24) stem loop, with one loxP site placed immediately downstream of the miR322 cluster stem loop. The construct was electroporated into 129P2/OlaHsd-derived E14 embryonic stem (ES) cells. Chimeric mice were crossed with a mouse of mixed genetic background (which incorporated FVB), then crossed to inbred C57BL/6 for two generations. Upon arrival, mice were bred to C57BL/6J for at least 1 generation to establish the colony.
Expressed Gene | lacZ, beta-galactosidase, E. coli |
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Site of Expression |
Allele Name | targeted mutation 1, Michael McManus |
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Allele Type | Targeted (Conditional ready (e.g. floxed), Reporter, Null/Knockout) |
Allele Synonym(s) | |
Gene Symbol and Name | Mirc24, microRNA cluster 24, including Mir351 through Mir322 |
Gene Synonym(s) | |
Expressed Gene | lacZ, beta-galactosidase, E. coli |
Strain of Origin | 129P2/OlaHsd |
Chromosome | X |
Molecular Note | A targeting vector was designed to insert an FRT site followed by a lacZ gene, a loxP site, a neomycin cassette, an FRT site and a loxP site (FRT-lacZ-loxP-Neo-FRT-loxP) upstream of the microRNA cluster stem loop and one loxP site immediately downstream of the microRNA stem loop |
While maintaining a live colony, these mice have been bred as heterozygotes, however, the Donating Investigator indicates that homozygotes are viable and fertile.
When using the STOCK Mirc24tm1Mtm/Mmjax mouse strain in a publication, please cite the originating article(s) and include MMRRC stock #36306 in your Materials and Methods section.
Facility Barrier Level Descriptions
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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