B6;D-Tg(Lfng-TagRFP/cre/ERT2)8Amc/J

Stock number: 017496
Research areas: Research Tools

Description

These Lfng-nuc-Tag-RFP-T-IRES-CE transgenic mice utilize a tamoxifen inducible Cre-mediated recombination system and may be useful in studies of genitourinary system development.

Detailed description

These Lfng-nuc-Tag-RFP-T-IRES-CE mutant mice have a tamoxifen inducible Cre-mediated recombination system and express the Tag-RFPT variant of Red Fluorescent Protein under the direction of the mouse Lfng, LFNG O-fucosylpeptide 3-beta-N-acetylglucosaminyltransferase, promoter. RFP fluorescence is not detected in wholemount embryos. Transgene expression is detected using immunohistochemistry in developing renal vesicles in the kidney of hemizygotes at 15.5 embryonic days of age. Tamoxifen inducible Cre recombinase activity is detected in a subset of Tag RFP-T positive cells in the kidney. Mice that are hemizygous for the knock in allele are viable, fertile, normal in size and do not display physical or behavioral abnormalities.

This strain was transferred from the collection of the GenitoUrinary Development Molecular Anatomy Project (GUDMAP).

Development

A targeting construct containing the Tag-RFP-T variant of Red Fluorescent Protein, cre/ERT2 and FRT site flanked Kanamycin/neomycin selection cassette was inserted into the consensus start ATG codon of the mouse Lfng gene in a BAC. The PGK-Neo/Kan cassette was removed by transient Flpe-recombinase expression. The resulting BAC transgene was microinjected into (C57BL/6 x DBA) F1 donor eggs. Founder line 8 was subsequently established. The mice were then crossed to C57BL/6 for two generations. Upon arrival at The Jackson Laboratory, the mice were crossed to C57BL/6J (Stock No. 000664) at least once to establish the colony.

Allele

Symbol: Tg(Lfng-TagRFP/cre/ERT2)8Amc
Name: transgene insertion 8, Andrew P McMahon
MGI accession ID: MGI:5317216
Generation method: Transgenic
Attributes: Recombinase-expressing; Reporter; Inducible
Marker symbol: Tg(Lfng-TagRFP/cre/ERT2)8Amc
Marker name: transgene insertion 8, Andrew P McMahon
Chromosome: UN
Strain of origin: (C57BL/6 x DBA)F1
Expressed genes: RFP,Red Fluorescent Protein,coral; cre/ERT2,Cre recombinase and estrogen receptor 1 (human) fusion gene,
Site of expression: Tamoxifen inducible Cre recombinase activity is detected in a subset of Tag RFP-T positive cells in the kidney.
Molecular note: A targeting construct containing the Tag-RFPT variant of Red Fluorescent Protein and Frt site flanked Kanamycin/neomycin selection cassette was inserted into the consensus start ATG codon of the mouse Lfng gene in a BAC. The PGK-Neo/Kan cassette was removed by transient Flpe-recombinase expression. The resulting BAC transgene was microinjected into (C57BL/6 x DBA) F1 donor eggs. Founder line 8 was subsequently established.