These Tagln-cre (also called Sm22aCre) transgenic mice express Cre recombinase under the control of the mouse smooth muscle protein 22-alpha (or transgelin) promoter. This strain represents an effective tool for generating tissue specific-targeted mutants that would be useful in studies of vascular diseases such as atherosclerosis.
Dr. Joachim Herz, Univ of Texas Southwest Med Ctr Dallas
Genetic Background | Generation |
---|---|
N18F16
|
Allele Type |
---|
Transgenic (Recombinase-expressing) |
Starting at:
$278.00 Domestic price for female 4-week |
356.51 Domestic price for breeder pair |
Mice hemizygous for the Tagln-cre (also called Sm22aCre) transgene are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. These transgenic mice express Cre recombinase under the control of the mouse smooth muscle protein 22-alpha (or transgelin) promoter. Cre recombinase expression (mRNA) closely patterns endogenous transgelin expression, with the highest levels detected in the aorta, intestine and uterus. Low levels of transcript are detected in all other organs tested, likely reflecting the vascular smooth muscle compartments in these tissues. Cre recombinase activity is observed in vascular smooth muscle cells of hepatic and pulmonary arteries, with no activity detected outside the vascular walls. When crossed with a strain containing a loxP site-flanked sequence of interest, Cre-mediated recombination results in deletion of the flanked sequence in vascular smooth muscle cells. This strain represents an effective tool for generating tissue specific-targeted mutants that would be useful in studies of vascular diseases such as atherosclerosis.
A transgenic construct containing cre coding sequence under the control of the mouse transgelin promoter was introduced into B6SJLF2 donor oocytes. Resulting offspring were bred to 129S5/SvEvBrd mice. These mice were then backcrossed to C57BL/6J mice for at least 15 generations. Upon arrival at The Jackson Laboratory, mice were bred to C57BL/6J (Stock No. 000664) for at least one generation to establish the colony.
Expressed Gene | cre, cre recombinase, bacteriophage P1 |
---|---|
Site of Expression | vascular smooth muscle cells |
Allele Name | transgene insertion 1, Joachim Herz |
---|---|
Allele Type | Transgenic (Recombinase-expressing) |
Allele Synonym(s) | SM; SM22a-Cre; Sm22alphaCre; SM22-Cre; SM22-Cretg; Tag1-Cre; Tgln-Cre |
Gene Symbol and Name | Tg(Tagln-cre)1Her, transgene insertion 1, Joachim Herz |
Gene Synonym(s) | |
Promoter | Tagln, transgelin, mouse, laboratory |
Expressed Gene | cre, cre recombinase, bacteriophage P1 |
Site of Expression | vascular smooth muscle cells |
Strain of Origin | (C57BL/6 x SJL)F2 |
Chromosome | UN |
General Note | Hemizygous transgenic mice are viable, fertile, normal in size, and do not display any gross physical or behavioral abnormalities. |
Molecular Note | The transgene consists of a Tagln (transgelin) promoter and a cre gene modified to include translational consensus and nuclear localization signals. The Tagln promoter was used to drive transgene expression in smooth muscle cells. Cre recombinase mRNA expression closely patterns endogenous transgelin expression, with the highest levels detected in the aorta, intestine, and uterus. Low levels of Cre recombinase transcript are detected in all other organs. Recombination occurs in vascular smooth muscle cells of hepatic and pulmonary arteries. No recombination has been detected in non-muscle cells. |
Mutations Made By | Dr. Joachim Herz, Univ of Texas Southwest Med Ctr Dallas |
When maintaining a live colony, hemizygous mice may be bred to wildtype (non-carrier) mice from the colony or to C57BL/6J inbred mice (Stock No. 000664).
When using the SM22-Cre mouse strain in a publication, please cite the originating article(s) and include JAX stock #017491 in your Materials and Methods section.
Service/Product | Description | Price |
---|---|---|
Hemizygous or Non Carrier for Tg(Tagln-cre)1Her |
Frozen Mouse Embryo | B6.Cg-Tg(Tagln-cre)1Her/J | $2595.00 |
Frozen Mouse Embryo | B6.Cg-Tg(Tagln-cre)1Her/J | $2595.00 |
Frozen Mouse Embryo | B6.Cg-Tg(Tagln-cre)1Her/J | $3373.50 |
Frozen Mouse Embryo | B6.Cg-Tg(Tagln-cre)1Her/J | $3373.50 |
Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
What information were you hoping to find through your search?
How easy was it to find what you were looking for?
We may wish to follow up with you. Enter your email if you are happy for us to connect and reachout to you with more questions.
Please Enter a Valid Email Address
Thank you for sharing your feedback! We are working on improving the JAX Mice search. Come back soon for exciting changes.