These Stra8-cre transgenic mice have expression of Cre recombinase (iCre) directed by the Stra8 genomic promoter fragment, and may be useful in generating conditional knockouts in postnatal, premeiotic, male germ cells for studying spermatogenesis.
Leonard P Guarente, Massachusetts Institute of Technology
Genetic Background | Generation |
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Allele Type |
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Transgenic (Recombinase-expressing) |
Homozygous Stra8-cre transgenic mice are viable and fertile, with expression of an optimized variant of Cre recombinase (iCre) directed by the 1.4 kb mouse Stra8 (stimulated by retinoic acid gene 8) genomic promoter fragment. Postnatal Cre-recombinase expression is first observed in testes at postnatal day (P)3 (early-stage spermatogonia), increases out to P7 (pre-leptotene-stage spermatocytes), and is not detected in other tissues examined (including ovaries). In addition, no cre expression is reported in male or female embryos. When Stra8-cre transgenic males are bred with female mice containing a loxP-flanked sequence, Cre-mediated recombination will result in deletion of the floxed sequence, specifically during these stages of spermatogenesis. The Donating Investigator has not attempted to make the congenic B6 strain homozygous. As Stra8-cre transgene expression is specific to developing sperm, transmission of the transgene must be through the male germline for experiments involving cre-induced recombination.
In an attempt to offer alleles on well-characterized or multiple genetic backgrounds, alleles are frequently moved to a genetic background different from that on which an allele was first characterized. This is the case for the strain above. It should be noted that the phenotype could vary from that originally described. We will modify the strain description if necessary as published results become available.
The 3.1 kb Stra8.iCre transgene was designed with the 1.4 kb mouse Stra8 (mouse stimulated by retinoic acid gene 8) genomic promoter
fragment (containing the entire promoter region from -1400 to +7), an SV40 splice donor/splice acceptor region, an optimized variant of Cre recombinase (iCre; improved with mammalian codon usage, removed putative cryptic splce sites, altered stop codon, and reduced CpG content to limit the chances of epigenetic silencing in mammals), and an SV40 polyA signal. The transgene was microinjected into the pronucleus of fertilized FVB eggs. The single founder male exhibiting germline expression of the transgene was generated. The mice were then backcrossed to C57BL/6J for at least 8 generations. Upon arrival, the mice were bred to C57BL/6J inbred mice (Stock No. 000664) to establish the colony.
Expressed Gene | cre, cre recombinase, bacteriophage P1 |
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Site of Expression | Postnatal Cre-recombinase expression is first observed in testes at postnatal day (P)3 (early-stage spermatogonia), increases out to P7 (pre-leptotene-stage spermatocytes), and is not detected in other tissues examined (including ovaries). In addition, no cre expression is reported in male or female embryos. |
Allele Name | transgene insertion 1, Robert E Braun |
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Allele Type | Transgenic (Recombinase-expressing) |
Allele Synonym(s) | Stra8-cre; Stra8-iCre; Tg(Stra8-iCre)1Reb |
Gene Symbol and Name | Tg(Stra8-icre)1Reb, transgene insertion 1, Robert E Braun |
Gene Synonym(s) | |
Promoter | Stra8, stimulated by retinoic acid gene 8, mouse, laboratory |
Expressed Gene | cre, cre recombinase, bacteriophage P1 |
Site of Expression | Postnatal Cre-recombinase expression is first observed in testes at postnatal day (P)3 (early-stage spermatogonia), increases out to P7 (pre-leptotene-stage spermatocytes), and is not detected in other tissues examined (including ovaries). In addition, no cre expression is reported in male or female embryos. |
Strain of Origin | FVB/NJ |
Chromosome | UN |
Molecular Note | A 1.4Kb promoter fragment of Stra8 was inserted upstream of the 1.26Kb improved Cre recombinase coding sequence, separated by SV40 splice donor/splice acceptor sequence. The Stra8 promoter region used was -1400 to +7, inserted into the plasmid vector with AvrII and XhoI recognition sites, respectively. |
Mutations Made By | Robert Braun, The Jackson Laboratory |
When maintaining a live colony, hemizygous mice may be bred together, to wildtype siblings, or to C57BL/6J inbred mice (Stock No. 000664). The Donating Investigator has not attempted to make the congenic B6 strain homozygous. As Stra8-cre transgene expression is specific to developing sperm, transmission of the transgene must be through the male germline for experiments involving cre-induced recombination.
When using the Stra8-iCre mouse strain in a publication, please cite the originating article(s) and include JAX stock #017490 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
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Hemizygous or Non carrier for Tg(Stra8-cre)1Reb |
Frozen Mouse Embryo | B6.FVB-Tg(Stra8-icre)1Reb/LguJ Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6.FVB-Tg(Stra8-icre)1Reb/LguJ Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6.FVB-Tg(Stra8-icre)1Reb/LguJ Frozen Embryo | $3373.50 |
Frozen Mouse Embryo | B6.FVB-Tg(Stra8-icre)1Reb/LguJ Frozen Embryo | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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