These Reg3g knock-out mice are useful in studies of bacterial colonization of the small intestine and host intestinal-microbial homeostasis.
Lora V. Hooper, UT Southwestern Medical Center, HHMI
Reg3g (regenerating islet-derived 3 gamma) is a bactericidal C-type lectin that specifically targets Gram-positive bacteria. Its expression is controlled by Myd88 in specialized epithelial cells of the small intestine.
These mice carry a targeted deletion of all six exons of the Reg3g gene. Homozygotes are born at predicted Mendelian ratios, appear healthy, and show no signs of enteropathy by histology. Mice deficient in this protein exhibit increased bacterial adherence to the small intestinal epithelial surface, increased fecal immunoglobulin A (IgA), and increased numbers of intestinal T helper 1 (TH1) cells. This strain has been useful in studies of bacterial colonization of the small intestine and host intestinal-microbial homeostasis.
A targeting vector was used to replace a 2.6 kb region encoding all six exons of the targeted gene with a FRT-flanked PGK-neomycin resistance cassette. The mutation was created in 129S6/SvEvTac-derived SM1 embryonic stem (ES) cells. Resultant mice were crossed with a C57BL/6 background FLP recombinase strain to excise the neomycin cassette. This strain was backcrossed to C57BL/6J for ten generations by the donating lab.
|Allele Name||targeted mutation 1.1, Lora Hooper|
|Allele Type||Targeted (Null/Knockout)|
|Gene Symbol and Name||Reg3g, regenerating islet-derived 3 gamma|
|Strain of Origin||129|
|Molecular Note||A targeting vector was used to replace a 2.6 kb region encoding all six exons of the targeted gene with a FRT-flanked PGK-neomycin resistance cassette. Flp-mediated recombination removed the neo cassette. The absence of transcript expression was confirmed by quantitative RT-PCR on ileum extracts.|
Heterozygotes or homozygotes may be bred.
When using the Reg3g knock-out mouse strain in a publication, please cite the originating article(s) and include JAX stock #017480 in your Materials and Methods section.