These Ibsp-topaz mice may be useful for visualizing and studying cell of the osteoblast and mature chondrocytes.
Peter Maye, University of Connecticut Health Center
Genetic Background | Generation |
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Allele Type |
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Transgenic (Reporter) |
Mice hemizygous for the Ibsp-topaz transgene are viable and fertile with the topaz variant of yellow fluorescent protein (YFP) expression directed by the integrin binding sialoprotein (Ibsp) promoter/enhancer regions from the BAC transgene. The transgene also contains the DMP1-mCherry and Trap-cyan reporters, however these reporters are not functional in this animal model. IBSP is a structural protein of the bone matrix and is produced by skeletal-associated cell types. These mice express topaz in osteocytes and hypertrophic chondrocytes.
Bacterial artificial chromosome (BAC) library (CHORI) was used to obtain a BAC (#RP23-395M11) containing the acid phosphatase 5, tartrate resistant (Acp5) gene. Another BAC library was used to obtain BAC #RP23-412O5, which contained the integrin binding sialoprotein (Ibsp) gene and the
dentin matrix protein 1 (Dmp1) gene. These genomic fragments were linked together and different fluorescent reporters, followed by polyadenylation sequences, were inserted upstream of the translational start site of each gene.
The cyan variant of green fluorescent protein (GFP) was inserted into Acp5, the topaz variant of yellow fluorescent protein (YFP) was inserted into Ibsp, and mCherry varient of red fluorescent protein (RFP) was inserted into Dmp1. This modified construct was microinjected into fertilized CD1 oocytes. Offspring from founder line 1 were bred to CD1 mice to establish a colony, and were subsequently backcrossed to CD1 for at least 18 generations. Upon arrival at The Jackson Laboratory, mice were bred to C57BL/6 inbred mice (Stock No. 000664) for at least one generation.
Expressed Gene | GFP, Green Fluorescent Protein, |
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Expressed Gene | YFP, Yellow Fluorescent Protein, jellyfish |
Expressed Gene | RFP, Red Fluorescent Protein, coral |
Site of Expression | These mice express topaz in osteocytes and hypertrophic chondrocytes. The DMP1-mCherry and Trap-cyan reporters are not functional. |
Allele Name | transgene insertion 1, Peter Maye |
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Allele Type | Transgenic (Reporter) |
Allele Synonym(s) | Ibsp-topaz |
Gene Symbol and Name | Tg(Acp5-CFP,Ibsp-YFP,Dmp1-RFP)1Pmay, transgene insertion 1, Peter Maye |
Gene Synonym(s) | |
Promoter | Dmp1, dentin matrix protein 1, mouse, laboratory |
Promoter | Ibsp, integrin binding sialoprotein, mouse, laboratory |
Promoter | Acp5, acid phosphatase 5, tartrate resistant, mouse, laboratory |
Expressed Gene | GFP, Green Fluorescent Protein, |
Expressed Gene | YFP, Yellow Fluorescent Protein, jellyfish |
Expressed Gene | RFP, Red Fluorescent Protein, coral |
Site of Expression | These mice express topaz in osteocytes and hypertrophic chondrocytes. The DMP1-mCherry and Trap-cyan reporters are not functional. |
Strain of Origin | CD-1 |
Chromosome | UN |
Molecular Note | Bacterial artificial chromosome (BAC) library (CHORI) was used to obtain a BAC (#RP23-395M11) containing the acid phosphatase 5, tartrate resistant (Acp5) gene. Another BAC library was used to obtain BAC #RP23-412O5, which contained the integrin binding sialoprotein (Ibsp) gene and the dentin matrix protein 1 (Dmp1) gene. These genomic fragments were linked together and different fluorescent reporters, followed by polyadenylation sequences, were inserted upstream of the translational start site of each gene. The cyan variant of green fluorescent protein (GFP) was inserted into Acp5, the topaz variant of yellow fluorescent protein (YFP) was inserted into Ibsp, and mCherry varient of red fluorescent protein (RFP) was inserted into Dmp1. Lines 1 was generated in which only the YFP reporter is functional. |
Mutations Made By | Peter Maye, University of Connecticut Health Center |
When maintaining a live colony, hemizygous mice may be bred to wildtype (non-carrier) mice from the colony.
When using the STOCK Tg(Acp5-CFP,Ibsp-YFP,Dmp1-RFP)1Pmay/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #017472 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
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Hemizygous or Non carrier for Tg(Acp5-CFP,Ibsp-YFP,Dmp1-RFP)1Pmay |
Frozen Mouse Embryo | STOCK Tg(Acp5-CFP Ibsp-YFP Dmp1-RFP)1Pmay/J | $2595.00 |
Frozen Mouse Embryo | STOCK Tg(Acp5-CFP Ibsp-YFP Dmp1-RFP)1Pmay/J | $2595.00 |
Frozen Mouse Embryo | STOCK Tg(Acp5-CFP Ibsp-YFP Dmp1-RFP)1Pmay/J | $3373.50 |
Frozen Mouse Embryo | STOCK Tg(Acp5-CFP Ibsp-YFP Dmp1-RFP)1Pmay/J | $3373.50 |
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