| Allele Name | transgene insertion 1, Peter Maye |
| Allele Type | Transgenic (Reporter) |
| Allele Synonym(s) | Ibsp-topaz |
| Gene Symbol and Name | Tg(Acp5-CFP,Ibsp-YFP,Dmp1-RFP)1Pmay, transgene insertion 1, Peter Maye |
| Gene Synonym(s) | |
| Promoter | Dmp1, dentin matrix protein 1, mouse, laboratory |
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| Promoter | Ibsp, integrin binding sialoprotein, mouse, laboratory |
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| Promoter | Acp5, acid phosphatase 5, tartrate resistant, mouse, laboratory |
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| Expressed Gene | GFP, Green Fluorescent Protein, |
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| Expressed Gene | YFP, Yellow Fluorescent Protein, jellyfish |
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| Expressed Gene | RFP, Red Fluorescent Protein, coral |
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| Site of Expression | These mice express topaz in osteocytes and hypertrophic chondrocytes. The DMP1-mCherry and Trap-cyan reporters are not functional. |
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| Strain of Origin | CD-1 |
| Chromosome | UN |
| Molecular Note | Bacterial artificial chromosome (BAC) library (CHORI) was used to obtain a BAC (#RP23-395M11) containing the acid phosphatase 5, tartrate resistant (Acp5) gene. Another BAC library was used to obtain BAC #RP23-412O5, which contained the integrin binding sialoprotein (Ibsp) gene and the dentin matrix protein 1 (Dmp1) gene. These genomic fragments were linked together and different fluorescent reporters, followed by polyadenylation sequences, were inserted upstream of the translational start site of each gene. The cyan variant of green fluorescent protein (GFP) was inserted into Acp5, the topaz variant of yellow fluorescent protein (YFP) was inserted into Ibsp, and mCherry varient of red fluorescent protein (RFP) was inserted into Dmp1. Lines 1 was generated in which only the YFP reporter is functional. |
| Mutations Made By | Peter Maye, University of Connecticut Health Center |
