STOCK Tg(Acp5-CFP,Ibsp-YFP,Dmp1-RFP)1Pmay/J

Stock number: 017472
Research areas: Internal/Organ Research, Research Tools

Description

These Ibsp-topaz mice may be useful for visualizing and studying cell of the osteoblast and mature chondrocytes.

Detailed description

Mice hemizygous for the Ibsp-topaz transgene are viable and fertile with the topaz variant of yellow fluorescent protein (YFP) expression directed by the integrin binding sialoprotein (Ibsp) promoter/enhancer regions from the BAC transgene. The transgene also contains the DMP1-mCherry and Trap-cyan reporters, however these reporters are not functional in this animal model. IBSP is a structural protein of the bone matrix and is produced by skeletal-associated cell types. These mice express topaz in osteocytes and hypertrophic chondrocytes.

Development

Bacterial artificial chromosome (BAC) library (CHORI) was used to obtain a BAC (#RP23-395M11) containing the acid phosphatase 5, tartrate resistant (Acp5) gene. Another BAC library was used to obtain BAC #RP23-412O5, which contained the integrin binding sialoprotein (Ibsp) gene and the dentin matrix protein 1 (Dmp1) gene. These genomic fragments were linked together and different fluorescent reporters, followed by polyadenylation sequences, were inserted upstream of the translational start site of each gene. The cyan variant of green fluorescent protein (GFP) was inserted into Acp5, the topaz variant of yellow fluorescent protein (YFP) was inserted into Ibsp, and mCherry varient of red fluorescent protein (RFP) was inserted into Dmp1. This modified construct was microinjected into fertilized CD1 oocytes. Offspring from founder line 1 were bred to CD1 mice to establish a colony, and were subsequently backcrossed to CD1 for at least 18 generations. Upon arrival at The Jackson Laboratory, mice were bred to C57BL/6 inbred mice (Stock No. 000664) for at least one generation.

Allele

Symbol: Tg(Acp5-CFP,Ibsp-YFP,Dmp1-RFP)1Pmay
Name: transgene insertion 1, Peter Maye
MGI accession ID: MGI:5428124
Generation method: Transgenic
Attributes: Reporter
Marker symbol: Tg(Acp5-CFP,Ibsp-YFP,Dmp1-RFP)1Pmay
Marker name: transgene insertion 1, Peter Maye
Chromosome: UN
Strain of origin: CD-1
Expressed genes: GFP,Green Fluorescent Protein,; YFP,Yellow Fluorescent Protein,jellyfish; RFP,Red Fluorescent Protein,coral
Site of expression: These mice express topaz in osteocytes and hypertrophic chondrocytes. The DMP1-mCherry and Trap-cyan reporters are not functional.
Molecular note: Bacterial artificial chromosome (BAC) library (CHORI) was used to obtain a BAC (#RP23-395M11) containing the acid phosphatase 5, tartrate resistant (Acp5) gene. Another BAC library was used to obtain BAC #RP23-412O5, which contained the integrin binding sialoprotein (Ibsp) gene and the dentin matrix protein 1 (Dmp1) gene. These genomic fragments were linked together and different fluorescent reporters, followed by polyadenylation sequences, were inserted upstream of the translational start site of each gene. The cyan variant of green fluorescent protein (GFP) was inserted into Acp5, the topaz variant of yellow fluorescent protein (YFP) was inserted into Ibsp, and mCherry varient of red fluorescent protein (RFP) was inserted into Dmp1. Lines 1 was generated in which only the YFP reporter is functional.