hOC-GFPtpz transgenic mice express the topaz variant of green fluorescent protein under the direction of the human BGLAP promoter/enhancer sequence. Expression is observed in mature osteoblasts of well-developed bone nodules and in recently deposited bone matrix.
David W. Rowe, University of Connecticut Health Center
Genetic Background | Generation |
---|---|
|
Allele Type |
---|
Transgenic (Reporter) |
hOC-GFPtpz transgenic mice express the topaz variant of green fluorescent protein (GFP) under the direction of the human bone gamma carboxyglutamate protein (BGLAP) promoter/enhancer sequence. The donating investigator reports that homozygotes are viable. BGLAP, also called osteocalcin, is expressed in bone and dentin and plays a role in bone building and mineralization and in calcium ion homeostasis. Under control of the BGLAP promoter, these mice express topaz protein in mature osteoblasts within well-developed bone nodules and in recently deposited bone matrix. Specifically, topaz was seen on the endosteal surface and in cortical bone at 7 days of age and within the mineralizing area of the bone nodule by 15 days of age. By 1 month of age, expression is seen in osteoblasts, osteocytes, and within vascular regions in cortical and trabecular bone.
The hOC-GFPtpz transgene was designed with the topaz variant of green fluorescent protein (GFP) driven by 3.8 kb human bone gamma carboxyglutamate protein (BGLAP) promoter/enhancer sequence. The transgene was microinjected into fertilized C57BL/6 oocytes. Offspring from founder line 1 were bred to C57BL/6 mice. These mice were bred with C57BL/6 inbred mice for at least 18 generations. Upon arrival at The Jackson Laboratory, transgenic mice were bred to C57BL/6J inbred mice (Stock No. 000664) to establish the colony.
Expressed Gene | GFP, Green Fluorescent Protein, |
---|---|
Site of Expression | These mice express topaz protein in mature osteoblasts within well-developed bone nodules and in recently deposited bone matrix. Specifically, topaz was seen on the endosteal surface and in cortical bone at 7 days of age and within the mineralizing area of the bone nodule by 15 days of age. By 1 month of age, expression is seen in osteoblasts, osteocytes, and within vascular regions in cortical and trabecular bone. |
Allele Name | transgene insertion 1, David Rowe |
---|---|
Allele Type | Transgenic (Reporter) |
Allele Synonym(s) | hOC-GFPtpz |
Gene Symbol and Name | Tg(BGLAP-Topaz)1Rowe, transgene insertion 1, David Rowe |
Gene Synonym(s) | |
Promoter | BGLAP, bone gamma-carboxyglutamate protein, human |
Expressed Gene | GFP, Green Fluorescent Protein, |
Site of Expression | These mice express topaz protein in mature osteoblasts within well-developed bone nodules and in recently deposited bone matrix. Specifically, topaz was seen on the endosteal surface and in cortical bone at 7 days of age and within the mineralizing area of the bone nodule by 15 days of age. By 1 month of age, expression is seen in osteoblasts, osteocytes, and within vascular regions in cortical and trabecular bone. |
Strain of Origin | C57BL/6 |
Chromosome | UN |
Molecular Note | The hOC-GFPtpz transgene was designed with the topaz variant of green fluorescent protein (GFP) driven by the 3.8 kb human bone gamma carboxyglutamate protein (BGLAP) promoter/enhancer sequences. Line 1 was generated. |
Mutations Made By | David Rowe, University of Connecticut Health Center |
When maintaining a live colony, hemizygous mice may be bred to wildtype (non-carrier) mice from the colony.
When using the C57BL/6-Tg(BGLAP-Topaz)1Rowe/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #017469 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Hemizygous or Non carrier for Tg(BGLAP-Topaz)1Rowe |
Frozen Mouse Embryo | C57BL/6-Tg(BGLAP-Topaz)1Rowe/J | $2595.00 |
Frozen Mouse Embryo | C57BL/6-Tg(BGLAP-Topaz)1Rowe/J | $2595.00 |
Frozen Mouse Embryo | C57BL/6-Tg(BGLAP-Topaz)1Rowe/J | $3373.50 |
Frozen Mouse Embryo | C57BL/6-Tg(BGLAP-Topaz)1Rowe/J | $3373.50 |
Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
What information were you hoping to find through your search?
How easy was it to find what you were looking for?
We may wish to follow up with you. Enter your email if you are happy for us to connect and reachout to you with more questions.
Please Enter a Valid Email Address
Thank you for sharing your feedback! We are working on improving the JAX Mice search. Come back soon for exciting changes.