C57BL/6-Tg(BGLAP-Topaz)1Rowe/J

Stock number: 017469
Research areas: Internal/Organ Research, Research Tools

Description

hOC-GFPtpz transgenic mice express the topaz variant of green fluorescent protein under the direction of the human BGLAP promoter/enhancer sequence. Expression is observed in mature osteoblasts of well-developed bone nodules and in recently deposited bone matrix.

Detailed description

hOC-GFPtpz transgenic mice express the topaz variant of green fluorescent protein (GFP) under the direction of the human bone gamma carboxyglutamate protein (BGLAP) promoter/enhancer sequence. The donating investigator reports that homozygotes are viable. BGLAP, also called osteocalcin, is expressed in bone and dentin and plays a role in bone building and mineralization and in calcium ion homeostasis. Under control of the BGLAP promoter, these mice express topaz protein in mature osteoblasts within well-developed bone nodules and in recently deposited bone matrix. Specifically, topaz was seen on the endosteal surface and in cortical bone at 7 days of age and within the mineralizing area of the bone nodule by 15 days of age. By 1 month of age, expression is seen in osteoblasts, osteocytes, and within vascular regions in cortical and trabecular bone.

Development

The hOC-GFPtpz transgene was designed with the topaz variant of green fluorescent protein (GFP) driven by 3.8 kb human bone gamma carboxyglutamate protein (BGLAP) promoter/enhancer sequence. The transgene was microinjected into fertilized C57BL/6 oocytes. Offspring from founder line 1 were bred to C57BL/6 mice. These mice were bred with C57BL/6 inbred mice for at least 18 generations. Upon arrival at The Jackson Laboratory, transgenic mice were bred to C57BL/6J inbred mice (Stock No. 000664) to establish the colony.

Allele

Symbol: Tg(BGLAP-Topaz)1Rowe
Name: transgene insertion 1, David Rowe
MGI accession ID: MGI:5428121
Generation method: Transgenic
Attributes: Reporter
Marker symbol: Tg(BGLAP-Topaz)1Rowe
Marker name: transgene insertion 1, David Rowe
Chromosome: UN
Strain of origin: C57BL/6
Expressed genes: GFP,Green Fluorescent Protein,
Site of expression: These mice express topaz protein in mature osteoblasts within well-developed bone nodules and in recently deposited bone matrix. Specifically, topaz was seen on the endosteal surface and in cortical bone at 7 days of age and within the mineralizing area of the bone nodule by 15 days of age. By 1 month of age, expression is seen in osteoblasts, osteocytes, and within vascular regions in cortical and trabecular bone.
Molecular note: The hOC-GFPtpz transgene was designed with the topaz variant of green fluorescent protein (GFP) driven by the 3.8 kb human bone gamma carboxyglutamate protein (BGLAP) promoter/enhancer sequences. Line 1 was generated.