C57BL/6-Tg(Dmp1-Topaz)1Ikal/J

Stock number: 017467
Research areas: Internal/Organ Research, Research Tools

Description

These Dmp1-Topaz transgenic mice express the topaz variant of GFP under the direction of the mouse Dmp1 promoter. Expression is observed in adult osteocytes of trabecular and metaphyseal regions of long bones, and in the cortical area of the femur and calvarial bone.

Detailed description

Dmp1-Topaz transgenic mice express the topaz variant of green fluorescent protein (GFP) under the direction of the mouse dentin matrix protein 1 (Dmp1) promoter. The donating investigator reports that homozygotes are viable. DMP1 is expressed in diverse cells within bone and tooth tissue and is required for bone and dentin mineralization. Under control of Dmp1, these mice express topaz protein in adult osteocytes of trabecular and metaphyseal regions of long bones, and in the cortical area of the femur and calvarial bone. Some topaz expression is also seen in brain and kidney.

Development

The Dmp1-Topaz transgene was designed with the topaz variant of green fluorescent protein (GFP) driven by 7892 base pairs of the promoter, exon1, and part of exon 2 of the dentin matrix protein 1 (Dmp1) gene. The transgene was microinjected into fertilized C57BL/6 oocytes. Offspring were bred to C57BL/6J mice and founder line 1 was established. These mice were maintained on a C57BL/6J background. Upon arrival at The Jackson Laboratory, transgenic mice were bred to C57BL/6J inbred mice (Stock No. 000664) to establish the colony.

Allele

Symbol: Tg(Dmp1-Topaz)1Ikal
Name: transgene insertion 1, I Kalajzic
MGI accession ID: MGI:4353034
Generation method: Transgenic
Attributes: Reporter
Marker symbol: Tg(Dmp1-Topaz)1Ikal
Marker name: transgene insertion 1, I Kalajzic
Chromosome: UN
Strain of origin: C57BL/6
Expressed genes: GFP,Green Fluorescent Protein,
Site of expression: GFP expression is observed in adult osteocytes of trabecular and metaphyseal regions of long bones, and in the cortical area of the femur and calvarial bone.
Molecular note: A fragment containing 7892 bp of the promoter region of mouse Dmp1 as well as exon1, all of intron 1, and part of exon 2 was used to drive expression of the topaz variant of GFP. Expression was confirmed by fluorescent microscopy of tail clips from F1 progeny of the founder.