STOCK Tg(Col10a1-mCherry)3Pmay/J

Stock number: 017465
Research areas: Internal/Organ Research, Research Tools

Description

These Col10a1-mCherry transgenic mice express a mCherry protein in hypertrophic chondrocytes. They may be useful for studying chondrocyte development.

Detailed description

Mice hemizygous for the BAC Col10a1-mCherry transgene are viable and fertile with mCherry expression directed by the collagen, type X, alpha 1 (Col10a1) promoter/enhancer regions within the BAC transgene. COL10A1 is expressed in mature hypertrophic chondrocytes during endochondral ossification, but is absent in immature chondrocytes. These BAC mice express the mCherry protein in hypertrophic chondrocytes. Specifically, at E14.5 mCherry fluorescence is seen in the ribs adjacent to the spine and at the center of the cartilaginous anlagen in the developing forearm. At E17.5, Col10a1-mCherry expression is seen in zones of cartilage maturation in the appendicular, axial skeleton, and cranial base.

Development

Bacterial artificial chromosome (BAC) library (CHORI) was used to obtain a BAC (#RP23-192A7) containing the entire mouse collagen, type X, alpha 1 (Col10a1) gene. This BAC was altered to insert a monomeric red fluorescent protein, mCherry, upstream of the initiation codon of Col10a1. This modified BAC was microinjected into fertilized CD1 oocytes. Offspring from founder line 3 were bred to CD1 mice, and were subsequently backcrossed to CD1 for at least 10 generations. Upon arrival at The Jackson Laboratory, mice were bred to C57BL/6J inbred mice (Stock No. 000664) for at least one generation.

Allele

Symbol: Tg(Col10a1-mCherry)3Pmay
Name: transgene insertion 3, Peter Maye
MGI accession ID: MGI:5428042
Generation method: Transgenic
Attributes: Reporter
Marker symbol: Tg(Col10a1-mCherry)3Pmay
Marker name: transgene insertion 3, Peter Maye
Chromosome: UN
Strain of origin: CD-1
Expressed genes: RFP,Red Fluorescent Protein,coral
Molecular note: Bacterial artificial chromosome (BAC) library (CHORI) was used to obtain a BAC (#RP23-192A7) containing the entire mouse collagen, type X, alpha 1 (Col10a1) gene. This BAC was altered to insert a monomeric red fluorescent protein, mCherry, upstream of the initiation codon of Col10a1. Line 3 was generated.