In this strain, exon 1 of the Eph receptor A7 (Epha7) gene is deleted, abolishing gene expression. These mice may be useful for studying the development of retinocollicular projection.
Uwe Drescher, Kings College London
Genetic Background | Generation |
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|
Allele Type | Gene Symbol | Gene Name |
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Targeted (Null/Knockout) | Epha7 | Eph receptor A7 |
In this strain, exon 1 of the Eph receptor A7 (Epha7) gene is deleted, abolishing gene expression. Homozygotes are viable, fertile, normal in size, and do not display any gross physical or behavioral abnormalities. EPHA7 is a member of the Eph family of receptor tyrosine kinases and acts as a repellent during axonal growth, suppressing branching. EPHA7 is expressed as a gradient (anterior > posterior) in the superior colliculus (SC). Homozygous Epha7-/- mice exhibit some wiring defects in brain, where topographic mapping of both temporal and nasal axons is disturbed. These mice may be useful for studying the development of retinocollicular projection.
A targeting vector was designed to replace exon 1 of the Eph receptor A7 (Epha7) gene with a loxP-flanked neomycin resistance (neo) cassette. The construct was electroporated into (129X1/SvJ x 129S1/Sv)F1-Kitl<+>-derived R1 embryonic stem (ES) cells. Correctly targeted ES cells were transiently transfected with a pIC-Cre expression plasmid to delete the neo cassette. Resulting ES cells were injected into blastocysts and resulting chimeric males were bred with C57BL/6 females. Resulting offspring were bred to CD1 outbred mice to establish a colony. Upon arrival, mice were bred to C57BL/6J inbred mice (Stock No. 000664) for at least one generation.
Allele Name | targeted mutation 1, Uwe Drescher |
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Allele Type | Targeted (Null/Knockout) |
Allele Synonym(s) | Epha7- |
Gene Symbol and Name | Epha7, Eph receptor A7 |
Gene Synonym(s) | |
Strain of Origin | (129X1/SvJ x 129S1/Sv)F1-Kitl+ |
Chromosome | 4 |
Molecular Note | Upstream sequence and exon 1, which contains the start coding and signal peptide, was replaced by a loxP-flanked neomycin selection cassette, which subsequently was removed via cre-mediated recombination. Sucessful ablation of the gene was confirmed by RT-PCR, in situ hybridization and Western blot analyses. |
Mutations Made By | Uwe Drescher, Kings College London |
When maintaining a live colony, homozygous mice may be bred together.
When using the STOCK Epha7tm1Ud/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #017445 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous for Epha7<tm1Ud> |
Frozen Mouse Embryo | STOCK Epha7<tm1Ud>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | STOCK Epha7<tm1Ud>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | STOCK Epha7<tm1Ud>/J Frozen Embryo | $3373.50 |
Frozen Mouse Embryo | STOCK Epha7<tm1Ud>/J Frozen Embryo | $3373.50 |
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