This strain carries a conditional targeted mutation of the 2310016C08Rik gene (commonly called Hig2, hypoxia inducible gene 2). This strain may be helpful in further characterization of the locus.
Jeremy Nathans, Johns Hopkins University
Genetic Background | Generation |
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|
Allele Type | Gene Symbol | Gene Name |
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Targeted (Null/Knockout) | Hilpda | hypoxia inducible lipid droplet associated |
The single exon of the 2310016C08Rik gene (commonly called Hig2, hypoxia inducible gene 2; GenBank AF141311) is flanked by loxP sites and followed by a membrane-tethered human placental alkaline phosphatase (hPLAP) in this conditional targeted mutation strain. Expression of the targeted gene is expected to be normal in the conditional allele. Following Cre-mediated recombination, the exon is excised enabling transcription of hPLAP under the control of the Hig2 promoter. hPLAP is expressed only when the Hig2 coding sequences are deleted by Cre-dependent excision. When crossed with a germline Cre strain (Sox2-Cre), expression of hPLAP was found to be widely expressed, with highest levels in the vasculature.
LoxP sites were inserted on either side of the single exon encoding the Hig2 gene in 129-derived embryonic stem (ES) cells. The open reading frame for a membrane tethered human placental alkaline phosphatase (hPLAP) was placed after the second loxP site. An Frt-flanked neomycin selectable marker used for ES cell selection (located just downstream of the alkaline phosphatase reporter) was removed by subsequent crossing to a mouse strain expressing Flp recombinase. This strain was maintained homozygously on a mixed C57BL/6 and 129 genetic background by the donating laboratory.
Allele Name | targeted mutation 1.1, Jeremy Nathans |
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Allele Type | Targeted (Null/Knockout) |
Allele Synonym(s) | |
Gene Symbol and Name | Hilpda, hypoxia inducible lipid droplet associated |
Gene Synonym(s) | |
Strain of Origin | 129 |
Chromosome | 6 |
Molecular Note | LoxP sites were inserted on either side of the single coding exon. The open reading frame for a membrane tethered human placental alkaline phosphatase (hPLAP) was placed after the second loxP site. An FRT-flanked neomycin selectable marker used for ES cell selection (located just downstream of the alkaline phosphatase reporter) was removed by flp-mediated recombination. |
Homozygous or heterozygous mice may be bred.
When using the STOCK Hilpdatm1.1Nat/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #017360 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous for Hilpda<tm1.1Nat> |
Frozen Mouse Embryo | STOCK Hilpda<tm1.1Nat>/J | $2595.00 |
Frozen Mouse Embryo | STOCK Hilpda<tm1.1Nat>/J | $2595.00 |
Frozen Mouse Embryo | STOCK Hilpda<tm1.1Nat>/J | $3373.50 |
Frozen Mouse Embryo | STOCK Hilpda<tm1.1Nat>/J | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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