STOCK Hilpda^tm1.1Nat/J

Stock number: 017360
Research areas: Research Tools

Description

This strain carries a conditional targeted mutation of the 2310016C08Rik gene (commonly called Hig2, hypoxia inducible gene 2). This strain may be helpful in further characterization of the locus.

Detailed description

The single exon of the 2310016C08Rik gene (commonly called Hig2, hypoxia inducible gene 2; GenBank AF141311) is flanked by loxP sites and followed by a membrane-tethered human placental alkaline phosphatase (hPLAP) in this conditional targeted mutation strain. Expression of the targeted gene is expected to be normal in the conditional allele. Following Cre-mediated recombination, the exon is excised enabling transcription of hPLAP under the control of the Hig2 promoter. hPLAP is expressed only when the Hig2 coding sequences are deleted by Cre-dependent excision. When crossed with a germline Cre strain (Sox2-Cre), expression of hPLAP was found to be widely expressed, with highest levels in the vasculature.

Development

LoxP sites were inserted on either side of the single exon encoding the Hig2 gene in 129-derived embryonic stem (ES) cells. The open reading frame for a membrane tethered human placental alkaline phosphatase (hPLAP) was placed after the second loxP site. An Frt-flanked neomycin selectable marker used for ES cell selection (located just downstream of the alkaline phosphatase reporter) was removed by subsequent crossing to a mouse strain expressing Flp recombinase. This strain was maintained homozygously on a mixed C57BL/6 and 129 genetic background by the donating laboratory.

Allele

Symbol: Hilpda^tm1.1Nat
Name: targeted mutation 1.1, Jeremy Nathans
MGI accession ID: MGI:5285399
Generation method: Targeted
Attributes: Null/Knockout
Marker symbol: Hilpda
Marker name: hypoxia inducible lipid droplet associated
Chromosome: 6
Strain of origin: 129
Molecular note: LoxP sites were inserted on either side of the single coding exon. The open reading frame for a membrane tethered human placental alkaline phosphatase (hPLAP) was placed after the second loxP site. An FRT-flanked neomycin selectable marker used for ES cell selection (located just downstream of the alkaline phosphatase reporter) was removed by flp-mediated recombination.