B6.Cg-Tg(tetO-Ppp3ca*)13967Kndl/J

Stock number: 017332
Research areas: Neurobiology Research, Research Tools

Description

TetO drives expression of the calcineurin (Ppp3ca, protein phosphatase 3, catalytic subunit, alpha isoform) autoinhibitory domain in these transgenic mice. When crossed with a tTA strain, transgene expression can be temporally regulated by doxycycline administration.

Detailed description

Calcineurin is a calcium and calmodulin-dependent serine/threonine protein phosphatase that is known to play a variety of biological roles in various organ systems and processes including signal transduction, working memory, social behavior, and antigen-specific immune processes.

These tansgenic mice carry the Ppp3ca (protein phosphatase 3, catalytic subunit, alpha isoform) C terminus autoinhibitory domain driven by the tetO promoter. When crossed with a tTA strain, transgene expression can be temporally regulated by doxycycline administration. The transgene does not appear to affect viability or fertility.

Neuronal expression of the transgene (e.g. via a Camk2a-tTA cross (see Stock No. 003010)) has multiple effects on synaptic plasticity and learning/memory behavior.

Development

The autoinhibitory domain of protein phosphatase 2B (calcineurin, Ppp3ca) was placed under the transcriptional control of the tetO promoter. This strain was created on a C57BL/6J x CBA F1 hybrid background and backcrossed to C57BL/6J for more than 6 generations by the donating laboratory.

Allele

Symbol: Tg(tetO-Ppp3ca*)13967Kndl
Name: transgene insertion 13967, Eric R Kandel
MGI accession ID: MGI:5289795
Generation method: Transgenic
Attributes: Inducible; Inserted expressed sequence
Marker symbol: Tg(tetO-Ppp3ca*)13967Kndl
Marker name: transgene insertion 13967, Eric R Kandel
Chromosome: UN
Strain of origin: (C57BL/6J x CBA)F1
Expressed genes: Ppp3ca,protein phosphatase 3, catalytic subunit, alpha isoform,mouse, laboratory
Molecular note: The autoinhibitory domain of protein phosphatase 2B (calcineurin, Ppp3ca) was placed under the transcriptional control of the tetO promoter.