TetO drives expression of the calcineurin (Ppp3ca, protein phosphatase 3, catalytic subunit, alpha isoform) autoinhibitory domain in these transgenic mice. When crossed with a tTA strain, transgene expression can be temporally regulated by doxycycline administration.
Eric R Kandel, Columbia University
Genetic Background | Generation |
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Allele Type |
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Transgenic (Inducible, Inserted expressed sequence) |
Calcineurin is a calcium and calmodulin-dependent serine/threonine protein phosphatase that is known to play a variety of biological roles in various organ systems and processes including signal transduction, working memory, social behavior, and antigen-specific immune processes.
These tansgenic mice carry the Ppp3ca (protein phosphatase 3, catalytic subunit, alpha isoform) C terminus autoinhibitory domain driven by the tetO promoter. When crossed with a tTA strain, transgene expression can be temporally regulated by doxycycline administration. The transgene does not appear to affect viability or fertility.
Neuronal expression of the transgene (e.g. via a Camk2a-tTA cross (see Stock No. 003010)) has multiple effects on synaptic plasticity and learning/memory behavior.
The autoinhibitory domain of protein phosphatase 2B (calcineurin, Ppp3ca) was placed under the transcriptional control of the tetO promoter. This strain was created on a C57BL/6J x CBA F1 hybrid background and backcrossed to C57BL/6J for more than 6 generations by the donating laboratory.
Expressed Gene | Ppp3ca, protein phosphatase 3, catalytic subunit, alpha isoform, mouse, laboratory |
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Site of Expression |
Allele Name | transgene insertion 13967, Eric R Kandel |
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Allele Type | Transgenic (Inducible, Inserted expressed sequence) |
Allele Synonym(s) | |
Gene Symbol and Name | Tg(tetO-Ppp3ca*)13967Kndl, transgene insertion 13967, Eric R Kandel |
Gene Synonym(s) | |
Promoter | tetO, tet operator, |
Expressed Gene | Ppp3ca, protein phosphatase 3, catalytic subunit, alpha isoform, mouse, laboratory |
Strain of Origin | (C57BL/6J x CBA)F1 |
Chromosome | UN |
Molecular Note | The autoinhibitory domain of protein phosphatase 2B (calcineurin, Ppp3ca) was placed under the transcriptional control of the tetO promoter. |
Mutations Made By | Eric Kandel, Columbia University |
Hemizygotes may be bred. The donating lab did not try to generate or maintain homozygotes
When using the B6.Cg-Tg(tetO-Ppp3ca*)13967Kndl/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #017332 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
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Hemizygous or Non carrier for Tg(tetO-Ppp3ca*)13967Kndl |
Frozen Mouse Embryo | B6.Cg-Tg(tetO-Ppp3ca*)13967Kndl/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6.Cg-Tg(tetO-Ppp3ca*)13967Kndl/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6.Cg-Tg(tetO-Ppp3ca*)13967Kndl/J Frozen Embryo | $3373.50 |
Frozen Mouse Embryo | B6.Cg-Tg(tetO-Ppp3ca*)13967Kndl/J Frozen Embryo | $3373.50 |
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