This aP2-Dgat1 transgenic strain exhibit increased adiposity when fed a high fat diet, but not white adipose tissue inflammation and insulin resistance. This mutant strain may be useful in studies of diet induced obesity, inflammatory macrophage activation, lipid-induced inflammation and lipid metabolism.
Robert V Farese, Jr., Harvard University School of Public Health
Genetic Background | Generation |
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Allele Type |
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Transgenic (Inserted expressed sequence) |
These transgenic mice express mouse Dgat1 (diacylglycerol O-acyltransferase 1), FLAG epitope amino-terminal tagged, under the control of the white adipose tissue specific mouse Fabp4 (fatty acid binding protein 4, adipocyte) promoter. In this founder line 2 (high expressing line) Dgat1 protein and mRNA levels are increased two fold in white apidose tissue and increased ~6?8.5 fold in peritoneal macrophages. Transgenic mice have adipocytes that are larger in size and mass than wildtype controls and elevated triglyceride levels in the reproductive fat pads. On either a chow or high fat diet, transgenic mice have a greater mean total fat pad weight than controls. When fed a high fat diet for 4 weeks, transgenic mice exhibit hyperphagy, higher body weight, elevated serum free fatty acid levels and lower serum triglyceride levels when compared to wildtype controls. Serum glucose and insulin levels, and liver and skeletal muscle triglyceride levels of transgenic and wildtype mice on the high fat diet are similar. Transgenic mice on a high fat diet for 16 weeks exhibit less triacylglycerol levels in liver and skeletal muscle, enhanced glucose and insulin tolerance, lower insulin and glucose fasting levels, and reduced macrophage recruitment into and inflammation of white adipose tissue. Mice hemizygous for the transgenic insert are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. The Donating Investigator has not attempted to make this strain homozygous.
A transgenic construct containing a 5.4-kb sequence of the mouse Fabp4, fatty acid binding protein 4, adipocyte, (or Ap2, adipocyte fatty acid binding protein) promoter upstream of a 1.6-kb FLAG epitope amino-terminal tagged cDNA of mouse Dgat1 (diacylglycerol O-acyltransferase 1) and a 2.2-kb human growth hormone minigene, was injected into fertilized C57BL/6 mouse eggs. Founder line 2 was subsequently established. The mice were maintained on the C57BL/6 background. Upon arrival at The Jackson Laboratory, the mice were crossed to C57BL/6J (Stock No. 000664) at least once to establish the colony.
Expressed Gene | Dgat1, diacylglycerol O-acyltransferase 1, mouse, laboratory |
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Site of Expression |
Allele Name | transgene insertion 2, Robert V Farese |
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Allele Type | Transgenic (Inserted expressed sequence) |
Allele Synonym(s) | aP2-Dgat1 |
Gene Symbol and Name | Tg(Fabp4-Dgat1)2Far, transgene insertion 2, Robert V Farese |
Gene Synonym(s) | |
Promoter | Fabp4, fatty acid binding protein 4, adipocyte, mouse, laboratory |
Expressed Gene | Dgat1, diacylglycerol O-acyltransferase 1, mouse, laboratory |
Strain of Origin | C57BL/6J |
Chromosome | UN |
Molecular Note | A 5.4 kb promoter for Fabp4 was used to drive expression of a 1.6 kb murine Dgat1 cDNA which expresses an amino-terminal FLAG epitope. The transgene is expressed only in white adepose tissue. A high expressing line (line 2) and a low expressing line (line 3) were established. |
Mutations Made By | Robert Farese, Jr., Harvard University School of Public Health |
When maintaining a live colony, these mice can be bred as hemizygotes. The Donating Investigator has not attempted to make this strain homozygous.
When using the C57BL/6-Tg(Fabp4-Dgat1)2Far/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #017321 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
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Hemizygous or Non carrier for Tg(Fabp4-Dgat1)2Far |
Frozen Mouse Embryo | C57BL/6-Tg(Fabp4-Dgat1)2Far/J | $2595.00 |
Frozen Mouse Embryo | C57BL/6-Tg(Fabp4-Dgat1)2Far/J | $2595.00 |
Frozen Mouse Embryo | C57BL/6-Tg(Fabp4-Dgat1)2Far/J | $3373.50 |
Frozen Mouse Embryo | C57BL/6-Tg(Fabp4-Dgat1)2Far/J | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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