These Irf5 (interferon regulatory factor 5) floxed mice have applications in studies related to innate immunity and autoimmunity.
Paula Pitha-Rowe, Johns Hopkins School of Medicine
Genetic Background | Generation |
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F?+N1F6
|
Allele Type | Gene Symbol | Gene Name |
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Targeted (Conditional ready (e.g. floxed), No functional change) | Irf5 | interferon regulatory factor 5 |
Starting at:
$278.00 Domestic price for female 4-week |
$2,854.50 Domestic price Cryo Recovery |
These mice possess loxP sites on either side of exon 2 of the targeted gene. Mice that are homozygous for this allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When these mutant mice are bred to mice that express Cre recombinase, resulting offspring will have exon 2 deleted in the cre-expressing tissue(s).
When bred to a strain with Cre recombinase expression in dendritic cells and B cells, this mutant mouse strain may be useful in studies of innate immunity and autoimmunity.
A targeting vector was used to flank exon 2 with loxP sites. This construct was electroporated into C57BL/6 derived B4 embryonic stem (ES) cells. Correctly targeted ES cells were injected into recipient blastocysts. The donating investigator reported that the resulting chimeric animals were backcrossed to wildtype C57BL/6 mice (see SNP note below). Upon arrival at The Jackson Laboratory, the mice were crossed to C57BL/6J (Stock No. 000664) at least once to establish the colony.
A 32 SNP (single nucleotide polymorphism) panel analysis, with 27 markers covering all 19 chromosomes and the X chromosome, as well as 5 markers that distinguish between the C57BL/6J and C57BL/6N substrains, was performed on the rederived living colony at The Jackson Laboratory Repository. While the 27 markers throughout the genome suggested a C57BL/6 genetic background, 2 of 5 markers that determine C57BL/6J from C57BL/6N were found to be segregating. These data suggest the mice sent to The Jackson Laboratory Repository were on a mixed C57BL/6J ; C57BL/6N genetic background.
Allele Name | targeted mutation 1, Paula Pitha-Rowe |
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Allele Type | Targeted (Conditional ready (e.g. floxed), No functional change) |
Allele Synonym(s) | |
Gene Symbol and Name | Irf5, interferon regulatory factor 5 |
Gene Synonym(s) | |
Strain of Origin | B6.Cg-Thy1a |
Chromosome | 6 |
Molecular Note | LoxP sites were inserted to flank exon 2, which encodes part of the DNA binding site. |
Mutations Made By | Paula Pitha-Rowe, Johns Hopkins School of Medicine |
When maintaining a live colony, these mice can be bred as homozygotes.
When using the IRF5 flox mouse strain in a publication, please cite the originating article(s) and include JAX stock #017311 in your Materials and Methods section.
Service/Product | Description | Price |
---|---|---|
Heterozygous for Irf5<tm1Ppr> |
Frozen Mouse Embryo | C57BL/6-Irf5<tm1Ppr>/J | $2595.00 |
Frozen Mouse Embryo | C57BL/6-Irf5<tm1Ppr>/J | $2595.00 |
Frozen Mouse Embryo | C57BL/6-Irf5<tm1Ppr>/J | $3373.50 |
Frozen Mouse Embryo | C57BL/6-Irf5<tm1Ppr>/J | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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