These Cd1d1 knock-out mice may be useful in the study of natural killer T (NKT) cells.
Albert Bendelac , University of Chicago
Genetic Background | Generation |
---|---|
|
Allele Type | Gene Symbol | Gene Name |
---|---|---|
Targeted (Null/Knockout) | Cd1d1 | CD1d1 antigen |
These mice carry a targeted knockout of the Cd1d1 (CD1d1 antigen) gene. Exons 2-6 were deleted, blocking expression of Cd1d1. Because the C57BL/6 Cd1d2 gene has a natural frameshift mutation that prevents its surface expression, deletion of Cd1d1 is sufficient to abolish all CD1D protein from the cell surface. Homozygotes are viable and fertile, and do not display any gross physical or behavioral abnormalities.
Exons 2-6 and an FRT-flanked neomycin cassette were flanked by loxP sites in an embryonic stem (ES) cell line of C57BL/6 origin. The neomycin selection cassette was excised from the resulting mice through crosses with a C57BL/6 background FLP strain. Exons 2-6 were excised through crosses with B6.C-Tg(CMV-cre)1Cgn/J mice (see Stock No. 006954) .
Allele Name | targeted mutation 1.2, Albert Bendelac |
---|---|
Allele Type | Targeted (Null/Knockout) |
Allele Synonym(s) | CD1.1 KO; Cd1d1/Cd1d2tm1.2Aben |
Gene Symbol and Name | Cd1d1, CD1d1 antigen |
Gene Synonym(s) | |
Strain of Origin | C57BL/6 |
Chromosome | 3 |
General Note | Because the C57BL/6 ES cells used to generate the allele contain a natural frameshift mutation in Cd1d2 that prevents its surface expression, deletion of Cd1d1 is sufficient to abolish all CD1D protein from the cell surface. |
Molecular Note | Cre-mediated recombination removed exons 2 through 6 of and the neo cassette. |
When using the CD1.1 KO mouse strain in a publication, please cite the originating article(s) and include JAX stock #017294 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous for Cd1d1/Cd1d2<tm1.2Aben> |
Frozen Mouse Embryo | B6(C)-Cd1d1<tm1.2Aben>/J | $2595.00 |
Frozen Mouse Embryo | B6(C)-Cd1d1<tm1.2Aben>/J | $2595.00 |
Frozen Mouse Embryo | B6(C)-Cd1d1<tm1.2Aben>/J | $3373.50 |
Frozen Mouse Embryo | B6(C)-Cd1d1<tm1.2Aben>/J | $3373.50 |
Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
What information were you hoping to find through your search?
How easy was it to find what you were looking for?
We may wish to follow up with you. Enter your email if you are happy for us to connect and reachout to you with more questions.
Please Enter a Valid Email Address
Thank you for sharing your feedback! We are working on improving the JAX Mice search. Come back soon for exciting changes.