These mice carry a targeted knockout of the Cd1d1 (CD1d1 antigen) gene. Exons 2-6 were deleted, blocking expression of Cd1d1. Because the C57BL/6 Cd1d2 gene has a natural frameshift mutation that prevents its surface expression, deletion of Cd1d1 is sufficient to abolish all CD1D protein from the cell surface. Homozygotes are viable and fertile, and do not display any gross physical or behavioral abnormalities.
Exons 2-6 and an FRT-flanked neomycin cassette were flanked by loxP sites in an embryonic stem (ES) cell line of C57BL/6 origin. The neomycin selection cassette was excised from the resulting mice through crosses with a C57BL/6 background FLP strain. Exons 2-6 were excised through crosses with B6.C-Tg(CMV-cre)1Cgn/J mice (see Stock No. 006954) .
|Allele Name||targeted mutation 1.2, Albert Bendelac|
|Allele Type||Targeted (Null/Knockout)|
|Allele Synonym(s)||CD1.1 KO; Cd1d1/Cd1d2tm1.2Aben|
|Gene Symbol and Name||Cd1d1, CD1d1 antigen|
|Strain of Origin||C57BL/6|
|General Note||Because the C57BL/6 ES cells used to generate the allele contain a natural frameshift mutation in Cd1d2 that prevents its surface expression, deletion of Cd1d1 is sufficient to abolish all CD1D protein from the cell surface.|
|Molecular Note||Cre-mediated recombination removed exons 2 through 6 of and the neo cassette.|